RNA interference analysis of the potential functions of cyclin-dependent kinase 2 in sexual reproduction of male oriental river prawns (Macrobrachium nipponense)

Wang, Pengchao; Zhang, Wenyi; Xiong, Yiwei; Chen, Tianyong; Jiang, Sufei; Qiao, Hui; Gong, Yongsheng; Wu, Yan; Jin, Shubo; Fu, Hongtuo

doi:10.1007/s10499-023-01112-9

Cited by 3 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In mammals and fishes, the SoxE family genes have shown their involvement in differentiation and development of testis [6,12], and in crustaceans, this function seems to be conserved. Our results showed that silencing of PtSoxE led to the downregulation of several reported spermatogenesis-related genes, including KIFC-1 [35], Vasa [36], CyclinB [37] and Cdc-2 [38], and vice versa when PtSoxE expression was activated, providing molecular evidence of the testicular development role of PtSoxE.…”

Section: Discussionmentioning

confidence: 51%

Molecular Characterization of a Male-specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone

Jiang¹,

Xu²,

Wang³

et al. 2023

Preprint

View full text Add to dashboard Cite

The Sry-related HMG-box (Sox) family is a group of transcriptional regulators that play a critical role in many important developmental processes in both vertebrates and invertebrates. In aquatic animals, the function of Sox genes on sexual development has been gained particular attentions. The present study reported the molecular characterization of a Sox member (PtSoxE) in the swimming crab, Portunus trituberculatus, and tissue distribution analysis showed it was male-specific. Since the most highly expression of PtSoxE was found in the androgenic gland (AG), its relation to the insulin-like androgenic gland hormone (IAG) was further investigated. The PtSoxE siRNA caused a significant decrease in IAG expression in both AG and testis, whereas PtSoxE expression could be induced by treating with AG homogenate and rIAG. The result suggested a transcriptional interaction between PtSoxE and IAG. In addition, PtSoxE expression showed a closely positive correlation with several reported spermatogenesis-related genes, suggesting its involvement in testicular development of P. trituberculatus.

show abstract

Section: Discussionmentioning

confidence: 51%

Molecular Characterization of a Male-specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone

Jiang¹,

Xu²,

Wang³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…RPS3 is reported to be phosphorylated by cyclindependent kinase 2 (Cdc2) during the G2/M phase. Cdc2 has been also identified to regulate the process of male reproduction in M. nipponense by RNAi [76]. The knockdown of the expression of PDHE1 changes the process of male reproduction in M. nipponense.…”

Section: Discussionmentioning

confidence: 99%

Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of Macrobrachium nipponense, Regulated by PDHE1, through Transcriptome Profiling Analysis

Jin,

Xiong,

Zhang

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

The previous publication identified that pyruvate dehydrogenase E1 (PDHE1) positively regulated the process of male reproduction in M. nipponense through affecting the expressions of insulin-like androgenic gland hormone. The present study aimed to identify the potential male-reproduction-related genes that were regulated by PDHE1 through performing the transcriptome profiling analysis in the testis and androgenic gland after the knockdown of the expressions of PDHE1 by the injection of dsPDHE1. Both RNA-Seq and qPCR analysis identified the significant decreases in PDHE1 expressions in the testis and androgenic gland in dsPDHE1-injected prawns compared to those in dsGFP-injected prawns, indicating the efficiency of dsPDHE1 in the present study. Transcriptome profiling analysis identified 56 and 127 differentially expressed genes (DEGs) in the testis and androgenic gland, respectively. KEGG analysis revealed that the energy-metabolism-related pathways represented the main enriched metabolic pathways of DEGs in both the testis and androgenic gland, including pyruvate metabolism, the Citrate cycle (TCA cycle), Glycolysis/Gluconeogenesis, and the Glucagon signaling pathway. Thus, it is predicted that these metabolic pathways and the DEGs from these metabolic pathways regulated by PDHE1 may be involved in the regulation of male reproduction in M. nipponense. Furthermore, four genes were found to be differentially expressed in both the testis and androgenic gland, of which ribosomal protein S3 was down-regulated and uncharacterized protein LOC113829596 was up-regulated in both the testis and androgenic gland in dsPDHE1-injected prawns. The present study provided valuable evidence for the establishment of an artificial technique to regulate the process of male reproduction in M. nipponense.

show abstract

Molecular Characterization of a Male-Specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone

Jiang

Wang

et al. 2023

Fishes

View full text Add to dashboard Cite

The Sry-related HMG-box (Sox) family is a group of transcriptional regulators that play a critical role in many important developmental processes in both vertebrates and invertebrates. In aquatic animals, the function of Sox genes on sexual development has attracted particular attention. The present study reported on the molecular characterization of a Sox member (PtSoxE) in the swimming crab, Portunus trituberculatus, and tissue distribution analysis showed it was male-specific. Since the highest expression of PtSoxE was found in the androgenic gland (AG), its relation to the insulin-like androgenic gland hormone (IAG) was further investigated. The PtSoxE siRNA caused a significant decrease in IAG expression in both AG and testis, whereas PtSoxE expression could be induced by treating with AG homogenate and rIAG. The result suggested a transcriptional interaction between PtSoxE and IAG. In addition, PtSoxE expression showed a closely positive correlation with several reported spermatogenesis-related genes, suggesting its involvement in the testicular development of P. trituberculatus.

show abstract

RNA interference analysis of the potential functions of cyclin-dependent kinase 2 in sexual reproduction of male oriental river prawns (Macrobrachium nipponense)

Cited by 3 publications

References 43 publications

Molecular Characterization of a Male-specific <em>SoxE</em> Gene in the Swimming Crab, <em>Portunus trituberculatus</em>, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone

Molecular Characterization of a Male-specific <em>SoxE</em> Gene in the Swimming Crab, <em>Portunus trituberculatus</em>, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone

Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of Macrobrachium nipponense, Regulated by PDHE1, through Transcriptome Profiling Analysis

Molecular Characterization of a Male-Specific SoxE Gene in the Swimming Crab, Portunus trituberculatus, and Transcriptional Interaction with Insulin-like Androgenic Gland Hormone

Contact Info

Product

Resources

About