RNA Interference in Plant Protection from Fungal and Oomycete Infection

Максимов, И. В.; Avalbaev, A.M.; Бурханова, Г. Ф.

doi:10.1134/s0003683822100106

Cited by 2 publications

(7 citation statements)

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“…Not only do hormonal signaling pathways play an important role in the regulation of bacteria-mediated ISR developing on the priming pathway, the RNA interference system and sRNAs also play a regulatory role [34,77]. DCL and AGO proteins are the most important components of the RNA interference machinery in plant defense since sRNAs are generated by DCL and function through AGO to suppress target genes [21].…”

Section: Discussionmentioning

confidence: 99%

“…Previously, the influence of the ethylene signaling pathway on increasing the expression of the AGO2 gene during the development of resistance to viral infection was shown [79]. The AGO2 protein's role in enhancing the secretion of the PR1 protein, a marker of the SA signaling pathway, was revealed, which led to an increase in plant resistance to bacterial infection [34].…”

Section: Discussionmentioning

confidence: 99%

“…Although the role of miRNAs in plant defense against insects has been established, the mechanisms of action and mechanisms of regulation of RNAi have not been determined. Nevertheless, it has been established that phytohormones play a regulatory role in genome reprogramming in the processes of RNA interference and DNA (de)methylation during the development of priming [34,35]. Currently, it is recognized that many miRNAs are involved in the regulation of signaling and the synthesis of various phytohormones [36].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, it is recognized that many miRNAs are involved in the regulation of signaling and the synthesis of various phytohormones [36]. However, in the literature, there is scattered information on the participation of phytohormones in the functioning of the RNAi system during the development of plant immunity, especially in the regulation of bacteria-mediated priming [34,36].…”

Section: Introductionmentioning

confidence: 99%

“…PGPBs can be inducers of sRNA expression in plants by triggering ISR, but the mechanism of this process is still poorly understood [34]. Recent works have shown that some bacteria of the genus Bacillus can trigger ISR signaling by regulating the expression of microRNAs through the activation of hormonal signaling pathways [37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

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Bacillus subtilis 26D Triggers Induced Systemic Resistance against Rhopalosiphum padi L. by Regulating the Expression of Genes AGO, DCL and microRNA in Bread Spring Wheat

Rumyantsev,

Veselova,

Burkhanova

et al. 2023

Microorganisms

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Bacillus subtilis 26D is a plant growth-promoting endophytic bacteria capable of inducing systemic resistance through the priming mechanism, which includes plant genome reprogramming and the phenomenon of RNA interference (RNAi) and microRNA (miRNAs). The phloem-feeding insect bird cherry-oat aphid Rhopalosiphum padi L. is a serious pest that causes significant damage to crops throughout the world. However, the function of plant miRNAs in the response to aphid infestation remains unclear. The results of this work showed that B. subtilis 26D stimulated aphid resistance in wheat plants, inducing the expression of genes of hormonal signaling pathways ICS, WRKY13, PR1, ACS, EIN3, PR3, and ABI5. In addition, B. subtilis 26D activated the RNAi mechanism and regulated the expression of nine conserved miRNAs through activation of the ethylene, salicylic acid (SA), and abscisic acid (ABA) signaling pathways, which was demonstrated by using treatments with phytohormones. Treatment of plants with SA, ethylene, and ABA acted in a similar manner to B. subtilis 26D on induction of the expression of the AGO4, AGO5 and DCL2, DCL4 genes, as well as the expression of nine conserved miRNAs. Different patterns of miRNA expression were found in aphid-infested plants and in plants treated with B. subtilis 26D or SA, ethylene, and ABA and infested by aphids, suggesting that miRNAs play multiple roles in the plant response to phloem-feeding insects, associated with effects on hormonal signaling pathways, redox metabolism, and the synthesis of secondary metabolites. Our study provides new data to further elucidate the fine mechanisms of bacterial-induced priming. However, further extensive work is needed to fully unravel these mechanisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%