RNA interference-mediated control of Asian citrus psyllid, the vector of the huanglongbing bacterial pathogen

Xj, Yu; Killiny, Nabil

doi:10.1007/s40858-020-00356-7

Cited by 29 publications

(35 citation statements)

References 70 publications

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“…Thus, the endocytotic mechanism of dsRNA uptake may promote RNAi-mediated gene silencing in organisms. In ACP, the core components of clathrin-mediated endocytosis, namely the clathrin heavy chain and AP50, an AP-2-like protein, have been identified [17].…”

Section: Challenges In Rnai-mediated Silencing For Pest Controlmentioning

confidence: 99%

“…For most insects, the results of dsRNA injection delivery differed from those of the other delivery methods. dsRNA injection enables the delivery of a precise amount of dsRNA directly into the target tissues or the hemolymph, consequently achieving a high level of gene silencing [17,56,61]. The first contact before cellular uptake is either the hemolymph or the gut content [31], and these body fluids may act as the main causes of dsRNA instability due to the enzymatic activity of double-stranded ribonucleases (dsRNases) and the physiological pH driving enzymatic activity in these fluids [31,35].…”

Section: Dsrna Stability and Efficiencymentioning

confidence: 99%

“…This technology has been tested as a novel approach in different eukaryotic species and, thanks to its high specificity, may serve as a potent tool for crop protection by managing insect pests or controlling the spread of vector-borne diseases [14][15][16]. RNAi presents great potential for ACP management because most of the core machinery genes are present in insects [17]. Therefore, insect control can be achieved through the cultivation of genetically modified citrus plants or application of dsRNA-based pesticides, similar to the conventional ones [18].…”

Section: Introductionmentioning

confidence: 99%

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Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

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Citrus species, including sweet oranges, grapefruits, pomelos, and lemons, are the most widely cultivated trees and consumed fruits worldwide. In citrus orchard management, the control of Huanglongbing (HLB) disease and its insect vector Diaphorina citri (Asian citrus psyllid, ACP) represents a major global challenge. Consumers have been increasingly pushing the citrus production chain toward a more sustainable system, including stringent measures to prevent the use of chemical pesticides. In recent years, biotechnological advances have offered safe and environmentally friendly alternatives for crop production. Technologies such as RNA interference (RNAi)-mediated gene silencing have emerged as innovative tools for agricultural pest management. Here, we provide an overview of RNAi as a promising approach for ACP control and discuss the associated challenges. Despite the availability of specific silencing sequences aimed at a target gene of the insect pest, the uptake of double-stranded RNA is limited in hemipteran insects. In this context, improved delivery methods, stability maintenance, and RNAi response are considered the factors contributing to the increased effectiveness of exogenous RNAi against hemipteran pests. These approaches can serve as potential tools for efficient ACP control.

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Section: Challenges In Rnai-mediated Silencing For Pest Controlmentioning

confidence: 99%

Section: Dsrna Stability and Efficiencymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

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“…Research efforts to develop effective management strategies for CLas and CLso have strongly focused on reducing the psyllid population, preventing acquisition and transmission of the bacterial pathogen by using several strategies including: RNAi-based suppression of the psyllid (Hajeri et al, 2014;Taning et al, 2016;Andrade and Hunter, 2017;Ghosh et al, 2017Ghosh et al, , 2018NASEM, 2018;Dubrovina and Kiselev, 2019;Adeyinka et al, 2020;Dubrovina et al, 2020;Yu and Killiny, 2020;Kiselev et al, 2021); antisense oligos, ASO (FANA and Morpholinos) targeting psyllids, their endosymbionts, and bacteria (Wesolowski et al, 2011(Wesolowski et al, , 2013Pietri et al, 2014;Daly et al, 2017;Hunter et al, 2017aHunter et al, ,b, 2018bMetz et al, 2017a,b;Moulton and Moulton, 2017;Patel et al, 2017;Pelz-Stelinski et al, 2017;Hunter and Sinisterra-Hunter, 2018;Xue et al, 2018;Novopashina et al, 2019;Pelz-Stelinski, 2020;Sandoval-Mojica et al, 2020, 2021Hunter and Pelz-Stelinski, 2021); gene-targeting and editing CRISPR/Cas9 systems (Chaverra- Rodriguez et al, 2018;Chaverra Rodriguez et al, 2020;Hunter et al, 2018aHunter et al, , 2019aHunter and Tomich, 2019); insecticides (Tiwari et al, 2011;Grafton-Cardwell et al, 2013;Qureshi et al, 2014;…”

Section: Introductionmentioning

confidence: 99%

Improving Suppression of Hemipteran Vectors and Bacterial Pathogens of Citrus and Solanaceous Plants: Advances in Antisense Oligonucleotides (FANA)

Hunter

Cooper²,

Sandoval-Mojica

et al. 2021

Front. Agron.

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We report on the development, evaluation, and efficient delivery of antisense oligonucleotide FANA (2′-deoxy-2′-fluoro-arabinonucleotide) RNA-targeting technology into citrus trees and potato plants for management of bacterial pathogens and arthropod pests. The FANA ASO technology is a single nucleotide strand of 20–24 nt in length that incorporates 2′F- chemically modifications of nucleotides, along with a phosphorothioate backbone and modified flanking nucleotides, in their structure called “gapmers,” produced by AUM LifeTech., Inc. These unique modified structures of FANA “triggers” enables gymnotic activity that self-delivers into cells, moving systemically in treated plants and insects, with significant suppression of their RNA targets. Reported is the FANA suppression of two plant-infecting bacterium Candidatus Liberibacter asiaticus, CLas (in citrus trees), and C. Liberibacter solanacearum, CLso (in potato and tomato). The CLas pathogen is associated with huanglongbing (a.k.a. Citrus Greening Disease), which causes severe loss of citrus trees, threatening global citrus production. The CLas bacterium is transmitted during feeding by the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae). CLso causes Zebra-Chip disease in potato and is transmitted by the potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae). Infected citrus trees or potato plants were treated with aqueous FANA solutions applied as a soil drench, root-infusion, topical spray, tree trunk injection or by absorption into cuttings, detached leaves, and leaf disks. Plants showed significant reduction of each pathogen or symptom development in response to FANA treatments. Similarly, ingestion of FANA solutions designed specifically to CLas by insects via artificial diets produced significant titer reductions in infected citrus psyllid adults that resulted in reduction of CLas transmission. The unique properties of FANA ASO solves many of the problems of stability, cell entry, and binding affinity that plagues exogenous RNAi strategies. Breakthroughs in production methods are reducing costs enabling these ASO to expand beyond medical applications into agricultural treatments. Thus, FANA ASO may provide viable treatments in the response to crop pandemics, like huanglongbing in citrus that threatens global food production.

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“…The limitations in the availability of efficient, cost-effective strategies for the management of the vector and the disease prompted a large number of investigations for the exploitation of new technologies, and promising results were mainly obtained with RNAi-based approaches (Santos-Ortega & Killiny, 2018;Lu et al, 2019a;Lu et al, 2019b;Yu & Killiny, 2020).…”

Section: Introductionmentioning

confidence: 99%

The molecular interplay of the establishment of an infection – gene expression of Diaphorina citrigut andCandidatusLiberibacter asiaticus

Bento

Darolt

Merlin

et al. 2021

Preprint

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Author Summary Huanglongbing (HLB) or greening is an incurable disease causing severe damage to citrus production, making citrus industrial activity unsustainable in several countries around the world. HLB is caused by three species of Candidatus Liberibacter. Ca. L. asiaticus (CLas), vectored by the psyllid Diaphorina citri , is the prevalent species. Attempts to apply new technologies in the development of strategies for disease and pest management are been made. However, we still miss basic information on this system to efficiently apply the current technologies and envisage the implementation of new approaches for pest control, despite the relevant scientific contribution available. One major gap is regarded to the molecular interplay between CLas and its vector. We focused our attention in the molecular interplay occurring at the first relevant interaction of CLas and D. citri , represented by the gut barrier. We report the transcriptional activity of CLas during the invasion and establishment of the infection in the gut of the vector, as well as the transcriptional activity of the vector in response to the infection. We identified several host genes that are targeted and regulated by CLas as well as several CLas genes that are promising targets for the application of new management strategies.

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RNA interference-mediated control of Asian citrus psyllid, the vector of the huanglongbing bacterial pathogen

Cited by 29 publications

References 70 publications

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Improving Suppression of Hemipteran Vectors and Bacterial Pathogens of Citrus and Solanaceous Plants: Advances in Antisense Oligonucleotides (FANA)

The molecular interplay of the establishment of an infection – gene expression of Diaphorina citrigut andCandidatusLiberibacter asiaticus

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