RNA Interference-Mediated Repression of S6 Kinase 1 Impairs Root Nodule Development in Soybean

Um, Ji-Hyun; Kim, Sunghan; Kim, Yun‐Kyoung; Song, Seok-Bo; Lee, Suk-Ha; Verma, Desh Pal S.; Cheon, Choong-Ill

doi:10.1007/s10059-013-2315-8

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…Asterisks indicate statistically significant differences compared with EV control (*P < 0.05; Student's t test). Libault et al, 2009Libault et al, , 2010Um et al, 2013). We have previously shown that the expression of many of these genes is altered in the Gb-and Gg-RNAi lines .…”

Section: Expression Of a Subset Of Nodulation Marker Genes Correspondmentioning

confidence: 99%

Phosphorylation-Dependent Regulation of G-Protein Cycle during Nodule Formation in Soybean

Choudhury

Pandey

2015

Plant Cell

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ORCID IDs: 0000-0002-6738-3574 (S.R.C.); 0000-0002-5570-3120 (S.P.)Signaling pathways mediated by heterotrimeric G-protein complexes comprising Ga, Gb, and Gg subunits and their regulatory RGS (Regulator of G-protein Signaling) protein are conserved in all eukaryotes. We have shown that the specific Gb and Gg proteins of a soybean (Glycine max) heterotrimeric G-protein complex are involved in regulation of nodulation. We now demonstrate the role of Nod factor receptor 1 (NFR1)-mediated phosphorylation in regulation of the G-protein cycle during nodulation in soybean. We also show that during nodulation, the G-protein cycle is regulated by the activity of RGS proteins. Lower or higher expression of RGS proteins results in fewer or more nodules, respectively. NFR1 interacts with RGS proteins and phosphorylates them. Analysis of phosphorylated RGS protein identifies specific amino acids that, when phosphorylated, result in significantly higher GTPase accelerating activity. These data point to phosphorylation-based regulation of G-protein signaling during nodule development. We propose that active NFR1 receptors phosphorylate and activate RGS proteins, which help maintain the Ga proteins in their inactive, trimeric conformation, resulting in successful nodule development. Alternatively, RGS proteins might also have a direct role in regulating nodulation because overexpression of their phospho-mimic version leads to partial restoration of nodule formation in nod49 mutants.

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Section: Expression Of a Subset Of Nodulation Marker Genes Correspondmentioning

confidence: 99%

Phosphorylation-Dependent Regulation of G-Protein Cycle during Nodule Formation in Soybean

Choudhury

Pandey

2015

Plant Cell

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“…The early molecular events involved in nodule initiation are quite well understood 3 4 5 6 . Although some legume genes have been implicated in the late developmental stage of nodule development and/or bacteroid differentiation 7 8 9 10 11 12 and some studies have investigated the molecular mechanisms that regulate nodule development 13 14 , information on nodule-related genes in the middle or late developmental stage of nodule development is also limited.…”

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confidence: 99%

RNA-Seq analysis of nodule development at five different developmental stages of soybean (Glycine max) inoculated with Bradyrhizobium japonicum strain 113-2

Yuan

Chen

et al. 2017

Sci Rep

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Nodule development directly affects nitrogen fixation efficiency during soybean growth. Although abundant genome-based information related to nodule development has been released and some studies have reported the molecular mechanisms that regulate nodule development, information on the way nodule genes operate in nodule development at different developmental stages of soybean is limited. In this report, notably different nodulation phenotypes in soybean roots inoculated with Bradyrhizobium japonicum strain 113-2 at five developmental stages (branching stage, flowering stage, fruiting stage, pod stage and harvest stage) were shown, and the expression of nodule genes at these five stages was assessed quantitatively using RNA-Seq. Ten comparisons were made between these developmental periods, and their differentially expressed genes were analysed. Some important genes were identified, primarily encoding symbiotic nitrogen fixation-related proteins, cysteine proteases, cystatins and cysteine-rich proteins, as well as proteins involving plant-pathogen interactions. There were no significant shifts in the distribution of most GO functional annotation terms and KEGG pathway enrichment terms between these five development stages. A cystatin Glyma18g12240 was firstly identified from our RNA-seq, and was likely to promote nodulation and delay nodule senescence. This study provides molecular material for further investigations into the mechanisms of nitrogen fixation at different soybean developmental stages.

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“…The transcription of the gene target of rapamycin ( GmTOR ) and its key downstream effector, GmS6K1 , were activated during nodulation. When GmS6K1 was knocked down, nodule development was severely impaired, suggesting an important role for the rapamycin pathway in nodule development (Um et al ., 2013). GmVTL1a , which function as a transporter of ferrous iron from the infected root cell cytosol to the symbiosome, moved iron across the symbiosome membrane to bacteria’s and played a crucial role in nitrogen fixation (Brear et al ., 2020; Liu et al ., 2020d).…”

Section: Genes Responsible For Important Agronomic Traitsmentioning

confidence: 99%

Progress in soybean functional genomics over the past decade

Zhang

Liu

Wang

et al. 2021

Plant Biotechnology Journal

105

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Summary Soybean is one of the most important oilseed and fodder crops. Benefiting from the efforts of soybean breeders and the development of breeding technology, large number of germplasm has been generated over the last 100 years. Nevertheless, soybean breeding needs to be accelerated to meet the needs of a growing world population, to promote sustainable agriculture and to address future environmental changes. The acceleration is highly reliant on the discoveries in gene functional studies. The release of the reference soybean genome in 2010 has significantly facilitated the advance in soybean functional genomics. Here, we review the research progress in soybean omics (genomics, transcriptomics, epigenomics and proteomics), germplasm development (germplasm resources and databases), gene discovery (genes that are responsible for important soybean traits including yield, flowering and maturity, seed quality, stress resistance, nodulation and domestication) and transformation technology during the past decade. At the end, we also briefly discuss current challenges and future directions.

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RNA Interference-Mediated Repression of S6 Kinase 1 Impairs Root Nodule Development in Soybean

Cited by 6 publications

References 26 publications

Phosphorylation-Dependent Regulation of G-Protein Cycle during Nodule Formation in Soybean

Phosphorylation-Dependent Regulation of G-Protein Cycle during Nodule Formation in Soybean

RNA-Seq analysis of nodule development at five different developmental stages of soybean (Glycine max) inoculated with Bradyrhizobium japonicum strain 113-2

Progress in soybean functional genomics over the past decade

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