RNA interference of the nicotine demethylase gene CYP82E4v1 reduces nornicotine content and enhances Myzus persicae resistance in Nicotiana tabacum L

Zhao, Dan; Qin, Lijun; Zhao, Degang

doi:10.1016/j.plaphy.2016.04.016

“…These pyridine alkaloids include anabasine, anatabine, nicotine and its de-methylated derivative nornicotine and they are characteristically found in leaves and/or roots of the ∼75 Nicotiana species that are naturally distributed in North and South America, mainland Australia, a few Pacific Islands and Southern Africa [5,6,44,45]. Among these pyridine alkaloids, the nicotine is the most predominant alkaloid found in cultivated tobacco varieties, typically accounting for 90%-95% of the total alkaloid pool [10].…”

Section: Discussionmentioning

confidence: 99%

“…It is generally accepted that these pyridine alkaloids serve as defensive compounds against herbivores, both within the context of native plant habitats [7][8][9]. Among these alkaloids, nicotine is the most predominant alkaloid found in a majority of tobacco varieties, typically accounting for 90% to 95% of the total alkaloid pool while another alkaloid is a relatively minor alkaloid, comprising about 2% to 5% in the mature leaf [10]. Besides, 230 digestion in insects [9] and is used as insecticides in agricultural applications [11].…”

Section: Introductionmentioning

confidence: 99%

Interference of Quinolinate Phosphoribosyltransferase Gene QPT Affects Agronomic Traits and Leaf Quality in Nicotiana tabacum L.

Wei¹,

Du²,

Qin³

et al. 2020

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Quinolinate phosphoribosyltransferase (QPRTase), a key enzyme in ensuring nicotinic acid is available for the synthesis of defensive pyridine alkaloids in Nicotiana species, also plays an important role in nicotinamide adenine dinucleotide (NAD) biosynthesis. In this study, the morphological traits, the quality characteristics and photosynthetic parameters in QPT-overexpressing/interfering tobacco plants were investigated, respectively. Results showed that the interference of QPT gene not only reduced significantly the morphological traits including plant height, stem girth, leaf number and leaf length, etc. at 20 days after transplanting (DAT), but the flowering period was delayed 10-15 d in interfered tobaccos compared with the overexpressed, control and wild-type counterparts. However, at 40 DAT and 60 DAT, only three indexes (plant height, stem girth and leaf number) in QPT-interfering plants appeared significant difference in comparison with other three types of tobacco lines. Meanwhile, the determination results from nicotine, sugar, K + and Clcontent showed the nicotine content in interfered plants was always significantly lower than that in overexpressed plants, control and the wild-type ones respectively whatever toppling or not. At the same time, the toppling treatment also caused the increasement of K + content among the four different tobacco lines, but the maximum increase amplitude of K + content was found in QPT-overexpressing tobaccos while the minimum appeared in QPT-interfering plants. Finally, QPT-interference in transgenic tobaccos likewise affected the photosynthesis by reducing net photosynthetic rate (Pn), stomata conductance (Gs) and transpiration rate (Tr), while there was no significant difference between QPT-overexpressing plants and the controls and the wild-types.

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“…This feedback loop regulation is considered being the only mechanism that controls the transcription of JAZs and JA-signaling pathway in all plants (Chini et al, 2007;Goossens et al, 2017;Howe et al, 2018). To date, based on those pathway and regulatory genes, on the one hand, antisense and RNAi have been reported to downregulate gene expression (such as BBL, ODC, QPT, and nicotine demethylase gene) to reduce nicotine, nornicotine, or TSNAs in leaves (DeBoer et al, 2013;Lewis et al, 2015;Xie et al, 2004b;Zhao et al, 2016). On the other hand, these multiple successes also revealed a challenge that the reduction of nicotine was traded with the increase of anatabine and other alkaloids (Chintapakorn and Hamill, 2003;DeBoer et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

ADe Novoregulation design shows an effectiveness in altering plant secondary metabolism

Li

¹

,

He

²

,

Hovary

³

et al. 2020

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Decrease of nicotine, other tobacco alkaloids (OTAs), and carcinogenic tobacco specific nitrosamines (TSNAs) is important for health. To reduce these compounds, we selected MRE and G-box elements of promoters of four Nicotiana tabacum Jasmonate ZIM-domain (NtJAZ) repressor genes as targets to screen transcription factors (TFs) and then identified Arabidopsis Production of Anthocyanin Pigment 1 (PAP1) and Transparent Testa 8 (TT8) for a novel regulation design. Electrophoretic mobility shift, cross-linked chromatin immunoprecipitation, and dual luciferase analyses demonstrated that PAP1, TT8, PAP1/TT8, and the PAP1/TT8/Transparent Testa Glabra 1 (TTG1) complex bound to and activated NtJAZ promoters. Stacked PAP1 (P) and TT8 (T), which were introduced to commercial tobacco cultivars, Narrow Leaf Madole (NL) and KY171 (KY), upregulated four NtJAZs, downregulated seven/eight nicotine biosynthetic genes, and reduced nicotine and nornicotine in transgenic red P+T-NL and P+L-KY plants. Field trials of red P+T-NL, P+L-KY, and PAP1 tobacco genotypes and metabolic quantification showed significant reduction of nicotine and four OTAs in most or all cured leaves. Three TSNAs and the fourth one were diminished by 63-92% and 30-74% in contents in all leaves of P+T-NL and P+L-KY genotypes, respectively. The total content of each TSNA was significantly reduced by 25-60% in leaves of PAP1 plants. Taken together, this regulation designed crossing from Arabidopsis anthocyanin to tobacco alkaloid pathway is defined as a distant pathway-cross regulation (DPCR). The proof of concept of DPCR unearths new functions of two known TFs, new activators of NtJAZs, and a negative regulation pathway of nicotine biosynthesis in red tobacco.

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“…It is generally accepted that these pyridine alkaloids serve as defensive compounds against herbivores, both within the context of native plant habitats [7][8][9]. Among these alkaloids, nicotine is the most predominant alkaloid found in a majority of tobacco varieties, typically accounting for 90% to 95% of the total alkaloid pool while another alkaloid is a relatively minor alkaloid, comprising about 2% to 5% in the mature leaf [10]. Besides, nicotine induces antifeed toxicity and hinders protein digestion in insects [9] and is used as insecticides in agricultural applications [11].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2020

JAST-B

0

View full text Add to dashboard Cite

Quinolinate phosphoribosyltransferase (QPRTase), a key enzyme in ensuring nicotinic acid is available for the synthesis of defensive pyridine alkaloids in Nicotiana species, also plays an important role in nicotinamide adenine dinucleotide (NAD) biosynthesis. In this study, the morphological traits, the quality characteristics and photosynthetic parameters in QPT-overexpressing/interfering tobacco plants were investigated, respectively. Results showed that the interference of QPT gene not only reduced significantly the morphological traits including plant height, stem girth, leaf number and leaf length, etc. at 20 days after transplanting (DAT), but the flowering period was delayed 10-15 d in interfered tobaccos compared with the overexpressed, control and wild-type counterparts. However, at 40 DAT and 60 DAT, only three indexes (plant height, stem girth and leaf number) in QPT-interfering plants appeared significant difference in comparison with other three types of tobacco lines. Meanwhile, the determination results from nicotine, sugar, K + and Clcontent showed the nicotine content in interfered plants was always significantly lower than that in overexpressed plants, control and the wild-type ones respectively whatever toppling or not. At the same time, the toppling treatment also caused the increasement of K + content among the four different tobacco lines, but the maximum increase amplitude of K + content was found in QPT-overexpressing tobaccos while the minimum appeared in QPT-interfering plants. Finally, QPT-interference in transgenic tobaccos likewise affected the photosynthesis by reducing net photosynthetic rate (Pn), stomata conductance (Gs) and transpiration rate (Tr), while there was no significant difference between QPT-overexpressing plants and the controls and the wild-types.

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RNA interference of the nicotine demethylase gene CYP82E4v1 reduces nornicotine content and enhances Myzus persicae resistance in Nicotiana tabacum L

Cited by 8 publications

References 30 publications

Interference of Quinolinate Phosphoribosyltransferase Gene QPT Affects Agronomic Traits and Leaf Quality in Nicotiana tabacum L.

Interference of Quinolinate Phosphoribosyltransferase Gene QPT Affects Agronomic Traits and Leaf Quality in Nicotiana tabacum L.

ADe Novoregulation design shows an effectiveness in altering plant secondary metabolism

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