RNA Isolation of  Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol.

Untoro, Yovilianda Maulitiva; Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Wardhani, Puspa; Aryati, Aryati; Ueda, Shuhai; Soegijanto, Soegeng

doi:10.20473/jkr.v3i1.7455

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…However, considering that these are environmental samples in which a large amount of targeted viral RNA was not expected, potential improvement of RNA isolation with the implementation of the formal, original protocol (O) set by the manufacturer was additionally tested with an alternative modified protocol (M). Untoro et al ( 29 ) conducted a similar study where they compared methanol, chloroform, and 2-isopropanol as alternative solvents to ethanol in the isolation of dengue virus type 2 RNA, and concluded that the used methanol and 2-isopropanol gave better results than ethanol. Generally speaking, the processing of environmental samples leads to a specific problem.…”

Section: Resultsmentioning

confidence: 99%

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Mlinar,

Kostelac,

Kovaček

et al. 2023

Food Technol. Biotechnol. (Online)

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Research background. While it is clear that SARS CoV-2 coronavirus is the primary respiratory virus, there are no entirely clarified ways of transmission. Foodborne transmission remained an unexplained path of transmission. Therefore, the goals of this paper are to examine and present an assessment of the most appropriate of the four selected kits for RNA extraction for the testing and detection of SARS-CoV-2 on food packaging and food surfaces. After this, it could be possible to indicate the possibility of infection through contact or direct food consumption. Experimental approach. Finding the best technique is vital as RNA extraction is one of the essential elements in detecting SARS-CoV-2. That was achieved through an experiment on four commercial kits with the original manufacturer protocol and with modification applications of ethanol and isopropanol. The selected kit was used for RNA extraction from the swab and the food samples. The coronavirus was then identified using real-time reverse transcription-polymerase chain reaction (RT-PCR) assays to determine whether the SARS-CoV-2 virus or viral particles are present in the food chain with the overall purpose of demonstrating the possibility that food can contribute as a vehicle for the transmission of the virus. Results and conclusions. The findings of this investigation made the most effective extraction kit and protocol stand out. The results of applicability confirmation of the kit indicated a significant share of positive samples on viral SARS-CoV-2 virus particles on surfaces. However, according to the second part of the study's findings, the virus was not detected in examined samples of food packaging surfaces, food surfaces, and in food. Novelty and scientific contribution. The study and presented results defined one of the most suitable protocols for isolating RNA from environmental surface samples. The main contribution of the study is in the presentation of the results of the study, that is, the examination of samples that are primarily related to the food chain, food packaging, food surfaces, and the food itself in the sense of ready-to-eat food. The results of this study could also be helpful for further determining the potential of food as a vector for the transmission of coronaviruses.

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Section: Resultsmentioning

confidence: 99%

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Mlinar,

Kostelac,

Kovaček

et al. 2023

Food Technol. Biotechnol. (Online)

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“…In this report, we have described (Untoro et al, 2018), DMSO, ethanol 96%, and acetone (Maharani et al, 2018 to A280. Pure RNA isolate has an A260/A280 ratio of 1.8 -2.1 (Farrel., 2005).…”

Section: Resultsmentioning

confidence: 99%

Variasi Presipitasi Pelarut pada Ekstraksi RNA dengan Metode Trizole dan Pengaruh terhadap Kemurnian RNA

Shaomianah¹

2020

MKH

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ABSTRAKVirus dengue ditularkan oleh nyamuk Aedes aegypti dan Aedes albopictus. Nyamuk-nyamuk tersebut endemik di sebagian besar daerah vektor-vektor itu muncul memiliki empat serotipe yang berbeda secara antigenik. Empat serotipe virus dengue: DENV-1, DENV-2, DENV-3 dan DENV-4. Penelitian ini bertujuan untuk mengetahui perbandingan pelarut yang baik terhadap virus Dengue RNA Surabaya tipe-1 (DENV-1). Pelarut yang digunakan adalah Isopropanol, dimethyl sulfoxide, 96% ethanol, methanol, acetone-Methanol (1: 1), dimethyl formamide, dan akuades. RNA diisolasi dengan metode TRIzol. TRIzol (atau TRI Reagent) adalah larutan satu fasa dari fenol dan guanidinium isothiocyanate yang secara bersamaan melarutkan bahan biologis dan mendenaturasi protein. Hasil dari penilitian ini adalah didapatkan pelarut presipitasi RNA terbaik adalah aseton-metanol (1:1), karena campuran pelarut tersebut memiliki konstanta dielektrik terendah. Studi ini menunjukkan bahwa pelarut aseton-metanol (1:1) dapat digunakan sebagai pelarut presipitasi untuk mengisolasi RNA

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RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol.

Cited by 2 publications

References 19 publications

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Variasi Presipitasi Pelarut pada Ekstraksi RNA dengan Metode Trizole dan Pengaruh terhadap Kemurnian RNA

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