RNA methylation in hematological malignancies and its interactions with other epigenetic modifications

Yao, Lan; Yin, Hua; Hong, Min; Wang, Yajun; Yu, Tingting; Teng, Yuanwen; Li, Tingting; Wu, Qirui

doi:10.1038/s41375-021-01225-1

Cited by 26 publications

(18 citation statements)

References 184 publications

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“…RNA modifications are abundant, diverse and ubiquitous. RNA modifications can regulate a variety of molecular processes including RNA splicing, translation, localization, binding to proteins or other RNAs [ 10 , 11 ]. RNA methylation is a posttranscriptional modification that exists in all organisms.…”

Section: Introductionmentioning

confidence: 99%

The role of m6A methylation in osteosarcoma biological processes and its potential clinical value

Wang

Shen

et al. 2022

Hum Genomics

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Osteosarcoma (OS) is the most common primary malignant bone tumor in children and young adults and has a poor prognosis. Recent developments in the field of high-throughput sequencing technology, particularly in methylated RNA immunoprecipitation sequencing (MeRIP-seq), have led to renewed interest in RNA methylation. Among the various RNA modifications, N6-methyladenosine (m6A) modifications are the most common. Emerging evidence suggests that m6A methylation can affect the complexity of cancer progression by regulating biological functions related to cancer. In this review, we will shed light on recent findings regarding the biological function of m6A methylation in OS and discuss future research directions and potential clinical applications of RNA methyltransferases in OS.

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Section: Introductionmentioning

confidence: 99%

The role of m6A methylation in osteosarcoma biological processes and its potential clinical value

Wang

Shen

et al. 2022

Hum Genomics

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“…The expression level of WTAP turned to be increased when the expression of METTL3 decreased. Most m6A regulators were considered to be dysregulated and play an essential role in leukemia ( Yao et al, 2021 ). Accumulating evidence indicated that m6A “writers,” METTL3, METTL14, and WTAP were all oncogenic in AML ( Qing et al, 2021b ).…”

Section: Discussionmentioning

confidence: 99%

N6-Methyladenosine-Related lncRNAs Are Novel Prognostic Markers and Predict the Immune Landscape in Acute Myeloid Leukemia

Zhang

Wen

et al. 2022

Front. Genet.

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Background: Acute myelocytic leukemia (AML) is one of the hematopoietic cancers with an unfavorable prognosis. However, the prognostic value of N 6-methyladenosine-associated long non-coding RNAs (lncRNAs) in AML remains elusive.Materials and Methods: The transcriptomic data of m6A-related lncRNAs were collected from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database. AML samples were classified into various subgroups according to the expression of m6A-related lncRNAs. The differences in terms of biological function, tumor immune microenvironment, copy number variation (CNV), and drug sensitivity in AML between distinct subgroups were investigated. Moreover, an m6A-related lncRNA prognostic model was established to evaluate the prognosis of AML patients.Results: Nine prognosis-related m6A-associated lncRNAs were selected to construct a prognosis model. The accuracy of the model was further determined by the Kaplan–Meier analysis and time-dependent receiver operating characteristic (ROC) curve. Then, AML samples were classified into high- and low-risk groups according to the median value of risk scores. Gene set enrichment analysis (GSEA) demonstrated that samples with higher risks were featured with aberrant immune-related biological processes and signaling pathways. Notably, the high-risk group was significantly correlated with an increased ImmuneScore and StromalScore, and distinct immune cell infiltration. In addition, we discovered that the high-risk group harbored higher IC50 values of multiple chemotherapeutics and small-molecule anticancer drugs, especially TW.37 and MG.132. In addition, a nomogram was depicted to assess the overall survival (OS) of AML patients. The model based on the median value of risk scores revealed reliable accuracy in predicting the prognosis and survival status.Conclusion: The present research has originated a prognostic risk model for AML according to the expression of prognostic m6A-related lncRNAs. Notably, the signature might also serve as a novel biomarker that could guide clinical applications, for example, selecting AML patients who could benefit from immunotherapy.

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“…As already discussed in the scientific literature and in our previous studies, UVRR is able to detect spectral modifications induced by changes in the nitrogenous bases chemical constitution [ 23 ]. This effect is suitable, for example, to detect epigenetic disorder induced by methylated nitrogenous bases in RNA [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Strategies and Perspectives for UV Resonance Raman Applicability in Clinical Analyses of Human Sperm RNA

Pachetti

D’Amico

Zupin

et al. 2021

IJMS

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Developing a deeper knowledge about the impact of DNA and RNA epigenetic mutations on sperm production and fertilization performance is essential for selecting best quality samples in Assisted Reproductive Technologies (ART). Indeed, sperm RNAs adenine and guanine are likely to be methylated in low quality RNA sperm samples and their study requires the employment of techniques able to isolate high quality nucleic acids. UV resonance Raman spectroscopy represents a valuable tool that is able to monitor peculiar molecular modifications occurring predominantly in nucleic acids, being less sensitive to the presence of other biological compounds. In this work, we used an UV Resonance Raman (UVRR) setup coupled to a synchrotron radiation source tuned at 250 nm, in order to enhance sperm RNAs adenine and guanine vibrational signals, reducing also the impact of a fluorescence background typically occurring at lower energies. Despite that our protocol should be further optimized and further analyses are requested, our results support the concept that UVRR can be applied for setting inexpensive tools to be employed for semen quality assessment in ART.

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RNA methylation in hematological malignancies and its interactions with other epigenetic modifications

Cited by 26 publications

References 184 publications

The role of m6A methylation in osteosarcoma biological processes and its potential clinical value

The role of m6A methylation in osteosarcoma biological processes and its potential clinical value

N6-Methyladenosine-Related lncRNAs Are Novel Prognostic Markers and Predict the Immune Landscape in Acute Myeloid Leukemia

Strategies and Perspectives for UV Resonance Raman Applicability in Clinical Analyses of Human Sperm RNA

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