RNA profile of immuno‐magnetically enriched lung cancer associated exosomes isolated from clinical samples

Singh, Shefali; Goyal, Deevanshu; Karthikeyan, Raman; Kumar, S. Udhaya; Malik, Prabhat Singh; Elangovan, Ravikrishnan

doi:10.1016/j.cancergen.2023.03.008

Cited by 3 publications

(1 citation statement)

References 58 publications

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“…Exosome-related gene expression status has been widely associated with tumor progression [5,6]. Therefore, the detection of specific mRNAs in exosomes may be used to detect cancer cells and to evaluate cancer progression [7,8].…”

Section: Introductionmentioning

confidence: 99%

RNA Profile of Cell Bodies and Exosomes Released by Tumorigenic and Non-Tumorigenic Thyroid Cells

Maggisano,

Capriglione,

Mio

et al. 2024

IJMS

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Tumor cells release exosomes, extracellular vesicle containing various bioactive molecules such as protein, DNA and RNA. The analysis of RNA molecules packaged in exosomes may provide new potential diagnostic or prognostic tumor biomarkers. The treatment of radioiodine-refractory aggressive thyroid cancer is still an unresolved clinical challenge, and the search for biomarkers that are detectable in early phase of the disease has become a fundamental goal for thyroid cancer research. By using transcriptome analysis, this study aimed to analyze the gene expression profiles of exosomes secreted by a non-tumorigenic thyroid cell line (Nthy-ori 3.1-exo) and a papillary thyroid cancer (TPC-1-exo) cell line, comparing them with those of cell bodies (Nthy-ori 3.1-cells and TPC-1-cells). A total of 9107 transcripts were identified as differentially expressed when comparing TPC-1-exo with TPC-1-cells and 5861 when comparing Nthy-ori 3.1-exo with Nthy-ori 3.1-cells. Among them, Sialic acid-binding immunoglobulin-like lectins 10 and 11 (SIGLEC10, SIGLEC11) and Keratin-associated protein 5 (KRTAP5-3) transcripts, genes known to be involved in cancer progression, turned out to be up-regulated only in TPC-1-exo. Gene ontology analysis revealed significantly enriched pathways, and only in TPC-1-exo were the differential expressed genes associated with an up-regulation in epigenetic processes. These findings provide a proof of concept that some mRNA species are specifically packaged in tumor-cell-derived exosomes and may constitute a starting point for the identification of new biomarkers for thyroid tumors.

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Section: Introductionmentioning

confidence: 99%

RNA Profile of Cell Bodies and Exosomes Released by Tumorigenic and Non-Tumorigenic Thyroid Cells

Maggisano,

Capriglione,

Mio

et al. 2024

IJMS

View full text Add to dashboard Cite

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Role of Exosomes in Parkinson’s and Alzheimer’s Diseases

Sharma,

Tyagi,

Chandra

et al. 2024

Exosomes Based Drug Delivery Strategies for Brain Disorders

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A comprehensive investigation of PRMT5 in the prognosis and ion channel features of lung cancer

Wang,

Chu,

et al. 2024

Front. Oncol.

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The increasing incidence and mortality associated with lung cancer (LC) is a significant global health challenge. The underlying mechanisms contributing to LC remain inadequately understood. However, emerging evidence suggests that the epigenetic modifier protein arginine methyltransferase 5 (PRMT5) plays a complex role in various cellular processes, including DNA repair, gene transcription, and alternative splicing, through its function in catalyzing the symmetric dimethylation of both histone and non-histone proteins. In this study, we examined the functional role of PRMT5 utilizing LC-related datasets (GSE30219, GSE50081, and TCGA LC cohort) through a series of analyses. Our findings revealed that PRMT5 was significantly overexpressed in LC samples compared to normal tissues and was correlated with overall survival and disease-free survival rates. Additionally, PRDM1 was identified as a key protein exhibiting a strong interaction with PRMT5. The prognostic model that integrated PRMT5 with clinical factors demonstrated robust performance in assessing survival outcomes. Elevated levels of PRMT5 were associated with poor prognosis in LC, as evidenced by analyses of the GSE30219, GSE50081, and TCGA-LC datasets. Furthermore, we identified 27 ion channel (IC) genes exhibited a correlation with PRMT5 in lung adenocarcinoma (LUAD), of which 9 genes were identified as statistically significant with KM survival analysis. Strikingly, all of the 9 genes, including LRRC8A, the same as PRMT5, were associated with poor prognosis in LUAD. Our research highlights the potential of PRMT5 as a novel prognostic biomarker and its relationship with IC genes in LC.

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RNA profile of immuno‐magnetically enriched lung cancer associated exosomes isolated from clinical samples

Cited by 3 publications

References 58 publications

RNA Profile of Cell Bodies and Exosomes Released by Tumorigenic and Non-Tumorigenic Thyroid Cells

RNA Profile of Cell Bodies and Exosomes Released by Tumorigenic and Non-Tumorigenic Thyroid Cells

Role of Exosomes in Parkinson’s and Alzheimer’s Diseases

A comprehensive investigation of PRMT5 in the prognosis and ion channel features of lung cancer

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