RNA reveals a succession of active fungi during the decay of Norway spruce logs

Rajala, Tiina; Peltoniemi, Mikko; Hantula, Jarkko; Mäkipää, Raisa; Pennanen, Taina

doi:10.1016/j.funeco.2011.05.005

Cited by 168 publications

(141 citation statements)

References 81 publications

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“…The fungal community dynamics in Picea deadwood observed in this study were distinctly different compared to those reported for boreal forest ecosystems in Fennoscandia (Kubartova et al 2012;Ovaskainen et al 2013;Rajala et al 2011Rajala et al , 2012. First, the ascomycetes that were reported to be most dominant during the early stages of decay in boreal forests (Rajala et al 2012) were largely absent in the Central European forests, where basidiomycetes were most abundant across all stages of decay.…”

Section: Fungal Diversity and Community Compositioncontrasting

confidence: 90%

“…The relatively low abundance of ECM in Picea logs from temperate forests as compared to boreal forests could be due to differences in N-availability in the two forest ecosystems (Nasholm et al 1998). We assume that ECM fungi in temperate forests preferentially acquire N from the soil, whereas in the N-limited boreal environment it is worthwhile for ECM fungi to acquire nitrogen by attacking deadwood (especially highly decayed deadwood) to avoid the competition with forest floor vegetation (Rajala et al 2011). …”

Section: Fungal Diversity and Community Compositionmentioning

confidence: 99%

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Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

et al. 2015

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Fungi play vital roles in the decomposition of deadwood due to their secretion of various enzymes that break down plant cell-wall complexes. The compositions of woodinhabiting fungal (WIF) communities change over the course of the decomposition process as the remaining mass of wood decreases and both abiotic and biotic conditions of the wood significantly change. It is currently not resolved which substrate-related factors govern these changes in WIF communities and whether such changes influence the deadwood decomposition rate. Here we report a study on fungal richness and community structure in deadwood of Norway spruce and European beech in temperate forest ecosystems using 454 pyrosequencing. Our aims were to disentangle the factors that correspond to WIF community composition and to investigate the links between fungal richness, taxonomically-resolved fungal identity, and microbial-mediated ecosystem functions and processes by analyzing physico-chemical wood properties, lignin-modifying enzyme activities and wood decomposition rates. Unlike fungal richness, we found significant differences in community structure between deadwood of different tree species. The composition of WIF communities was related to the physico-chemical properties of the deadwood substrates. Decomposition rates and the activities of ligninmodifying enzymes were controlled by the succession of the fungal communities and competition scenarios rather than fungal OTU richness. Our results provide further insights into links between fungal community structure and microbialmediated ecosystem functions and processes.

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Section: Fungal Diversity and Community Compositioncontrasting

confidence: 90%

Section: Fungal Diversity and Community Compositionmentioning

confidence: 99%

Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

et al. 2015

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“…The fast appearance-disappearance of fungal taxa seems to contrast with the reported persistence of DNA from inactive fungi in decaying wood (Rajala et al, 2011) and to support the rapid turnover of early/intermediate/late saprotrophs (Lindahl and Finlay, 2006). The successional changes are likely governed not only by the relatively slow changes of the polysaccharide, lignin and nitrogen content in litter but possibly by other factors including more subtle changes in litter chemistry and interspecific fungal interactions.…”

Section: Discussionmentioning

confidence: 90%

Fungal community on decomposing leaf litter undergoes rapid successional changes

2012

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Fungi are considered the primary decomposers of dead plant biomass in terrestrial ecosystems. However, current knowledge regarding the successive changes in fungal communities during litter decomposition is limited. Here we explored the development of the fungal community over 24 months of litter decomposition in a temperate forest with dominant Quercus petraea using 454-pyrosequencing of the fungal internal transcribed spacer (ITS) region and cellobiohydrolase I (cbhI) genes, which encode exocellulases, to specifically address cellulose decomposers. To quantify the involvement of phyllosphere fungi in litter decomposition, the fungal communities in live leaves and leaves immediately before abscission were also analysed. The results showed rapid succession of fungi with dramatic changes in the composition of the fungal community. Furthermore, most of the abundant taxa only temporarily dominated in the substrate. Fungal diversity was lowest at leaf senescence, increased until month 4 and did not significantly change during subsequent decomposition. Highly diverse community of phyllosphere fungi inhabits live oak leaves 2 months before abscission, and these phyllosphere taxa comprise a significant share of the fungal community during early decomposition up to the fourth month. Sequences assigned to the Ascomycota showed highest relative abundances in live leaves and during the early stages of decomposition. In contrast, the relative abundance of sequences assigned to the Basidiomycota phylum, particularly basidiomycetous yeasts, increased with time. Although cellulose was available in the litter during all stages of decomposition, the community of cellulolytic fungi changed substantially over time. The results indicate that litter decomposition is a highly complex process mediated by various fungal taxa.

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“…This might be explained by the reduced ability of ECM fungi to degrade plant cell wall polymers due to a decrease in the number of glycosyl hydrolases and other carbohydrate-acting enzymes (50,51) and due to species succession in decomposing wood (52).…”

Section: Discussionmentioning

confidence: 99%

Dominant Tree Species and Soil Type Affect the Fungal Community Structure in a Boreal Peatland Forest

Sun

Terhonen

Kovalchuk

et al. 2016

Appl Environ Microbiol

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f Boreal peatlands play a crucial role in global carbon cycling, acting as an important carbon reservoir. However, little information is available on how peatland microbial communities are influenced by natural variability or human-induced disturbances. In this study, we have investigated the fungal diversity and community structure of both the organic soil layer and buried wood in boreal forest soils using high-throughput sequencing of the internal transcribed spacer (ITS) region. We have also compared the fungal communities during the primary colonization of wood with those of the surrounding soils. A permutational multivariate analysis of variance (PERMANOVA) confirmed that the community composition significantly differed between soil types (P < 0.001) and tree species (P < 0.001). The distance-based linear models analysis showed that environmental variables were significantly correlated with community structure (P < 0.04). The availability of soil nutrients (Ca [P ‫؍‬ 0.002], Fe [P ‫؍‬ 0.003], and P [P ‫؍‬ 0.003]) within the site was an important factor in the fungal community composition. The species richness in wood was significantly lower than in the corresponding soil (P < 0.004). The results of the molecular identification were supplemented by fruiting body surveys. Seven of the genera of Agaricomycotina identified in our surveys were among the top 20 genera observed in pyrosequencing data. Our study is the first, to our knowledge, fungal high-throughput next-generation sequencing study performed on peatlands; it further provides a baseline for the investigation of the dynamics of the fungal community in the boreal peatlands.

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RNA reveals a succession of active fungi during the decay of Norway spruce logs

Cited by 168 publications

References 81 publications

Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests

Fungal community on decomposing leaf litter undergoes rapid successional changes

Dominant Tree Species and Soil Type Affect the Fungal Community Structure in a Boreal Peatland Forest

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