RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection

Xing, Dongxu; Yang, Qiong; Jiang, Liang; Li, Qingrong; Xiao, Yang; Ye, Mingqiang; Xia, Qingyou

doi:10.3390/ijms18020234

Cited by 24 publications

(17 citation statements)

References 52 publications

(53 reference statements)

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“…To date, the genome‐wide gene expression profiles on fungal entomopathogens challenges have been characterized in many insect species, such as Bombyx mori , G . mellonella , Melanotus cribricollis , Plutella xylostella and Riptortus pedestris 15–19 . However, few reports have explored the insect‐fungus interaction of BPH during M. anisopliae infection, which extremely impeded the genetic improvement of the fungal entomopathogen against BPH 20 …”

Section: Introductionmentioning

confidence: 99%

Comprehensive insights into host‐pathogen interaction between brown planthopper and a fungal entomopathogen by dual RNA sequencing

Wang

Pan

et al. 2021

Pest Management Science

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BACKGROUND The brown planthopper (BPH) is one of the most destructive pests of rice, causing tremendous yield and economic losses every year. The fungal entomopathogen Metarhizium anisopliae was previously proved to have great potential for BPH biocontrol. Genome‐wide insight into the insect‐fungus interaction is crucial for genetic improvement of M. anisopliae to enhance its virulence to BPH but still has been poorly explored. RESULTS Using dual RNA‐seq approach, we present here a global view of host and fungal gene expressions in BPH adults during the fungal infection. The results revealed that BPH could initiate strong defense responses against the fungal attack by upregulating the expressions of a large number of genes, including genes involved in cuticle formation, immune response, cell detoxification and biomacromolecule metabolism. Correspondingly, the fungal entomopathogen could induce a series of genes to infect and modulate BPH, including genes involved in fungal penetration, invasive growth, stress resistance and virulence. Three host defense‐related genes (NlPCE4, NlPOD1 and NlCYP4DE1) were chosen for further function analysis. RNAi‐mediated knockdown of NlPCE4 caused a significant decrease in BPH survival, but no obvious effects on the survival rates were detected by the suppression of NlPOD1 and NlCYP4DE1. Combination of dsRNA injection and fungal infection could significantly enhance the BPH‐killing speed, as synergistic mortalities were observed in co‐treatments of RNAi and M. anisopliae infection. CONCLUSION Our study provides a comprehensive insight into molecular mechanisms of host‐pathogen interaction between BPH and M. anisopliae and contributes to future development of new efficient biocontrol strategies for BPH biocontrol.

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Section: Introductionmentioning

confidence: 99%

Comprehensive insights into host‐pathogen interaction between brown planthopper and a fungal entomopathogen by dual RNA sequencing

Wang

Pan

et al. 2021

Pest Management Science

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“…For example, to prevent the adhesion of fungal conidia, Liposcelis bostrychophila produces unique cuticular fatty amides, while Nezara viridula secretes several cuticular lipids and aldehydes to defend against entomopathogenic fungi 21,22 . Furthermore, insects within the same species have distinct immune factors that play crucial roles in their immune responses to invading pathogens, such as genes encoding cuticle proteins, serine proteinase inhibitors (SPIs), and antimicrobial peptides (AMPs) from two silkworm strains (sensitive and resistant to B. bassiana ) upon B. bassiana infection 23 . Accordingly, under long‐term selection, insects have developed a series of strategies to evade or overcome fungal infections.…”

Section: Introductionmentioning

confidence: 99%

Immunotranscriptome analysis of Plutella xylostella reveals differences in innate immune responses to low‐ and high‐virulence Beauveria bassiana strain challenges

Wang

Zhou

Hanghang³

et al. 2020

Pest Management Science

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BACKGROUND Entomopathogenic fungi have developed multiple strategies to overcome the immune defenses of their target insects, whereas insect pests have devised various defense mechanisms to combat fungal infection. However, differences in the molecular mechanisms of the innate immune defense strategies of insects upon infection with different fungal strains from the same species have not been reported. RESULTS Two Beauveria bassiana strains were obtained that significantly varied in their pathogenicity but were comparable in terms of growth, conidial yield, and cuticle penetration. To investigate the molecular mechanisms underlying the immune response of Plutella xylostella infected with these two strains, RNA‐Seq was performed 48 h after infection. A total of 1027 differentially expressed genes (DEGs) were identified, and more than 200 DEGs were enriched in Kyoto Encyclopedia of Genes and Genome (KEGG) pathways involved in disease response, revealing differences in the immune response of P. xylostella to different B. bassiana infections at 48 h. Twenty‐eight of the DEGs were related to innate immune functions, such as pathogen recognition, immune system activation and antimicrobial reactions. RNA interference (RNAi)‐mediated gene silencing assays showed that PxApoLIII and PxCSP played critical roles in the P. xylostella immune response. PxApoLIII was expressed at higher levels during infection with the high‐virulence strain, whereas PxCSP showed the opposite expression pattern during infection with the low‐virulence strain, indicating that PxApoLIII and PxCSP might participate in P. xylostella innate immune defense against high‐ and low‐virulence B. bassiana strains. CONCLUSION The present findings demonstrate that strains of a single species of pathogenic fungi that differ in virulence can induce the expression of different genes in P. xylostella. These results advance our knowledge of the molecular mechanisms underlying fungi–pest interactions.

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“…For example, in a previous study [30], researchers analysed key genes involved in monacolin K biosynthesis of Monascus purpureus by de novo RNA-Seq, identifying nine genes as being potentially associated with this process. The molecular mechanisms underlying the responses to Beauveria bassiana infection in silkworm ( Bombyx mori ) were also investigated via transcriptome analysis [31,32] and the drug metabolism pathways involved in toxin detoxification were found to be related to B. bassiana resistance and susceptibility. Furthermore, numerous comparative transcriptomic studies in plants have compared developmental stages in a single species across different tissues [33].…”

Section: Introductionmentioning

confidence: 99%

Identification of cordycepin biosynthesis-related genes through de novo transcriptome assembly and analysis in Cordyceps cicadae

Liu

Yao

et al. 2018

R. Soc. open sci.

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Cordyceps cicadae (Chanhua) is a parasitic fungus that grows on Cicada flammata larvae and is used to relieve exhaustion and treat numerous diseases, in part through its active constituent, cordycepin. We used de novo Illumina HiSeq 4000 sequencing to obtain transcriptomes of C. cicadae mycelium, fruiting body, and sclerotium, and identify differentially expressed genes. In the mycelium versus sclerotium libraries, 1576 upregulated and 2300 downregulated genes were identified. In the mycelium versus fruiting body and fruiting body versus sclerotium body libraries, 1604 and 1474 upregulated and 1365 and 1320 downregulated genes, respectively, were identified. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses identified 19 genes differentially expressed in mycelium versus fruiting body as related to the purine pathway, along with 28 and 16 genes differentially expressed in the mycelium versus sclerotium and fruiting body versus sclerotium groups, respectively. Gene expression of six key enzymes was validated by quantitative polymerase chain reaction. Specifically, 5′-nucleotidase (c62060g1) and adenosine deaminase (c35629g1) in purine nucleotide metabolism, which are involved in cordycepin biosynthesis, were significantly upregulated in the sclerotium group. These findings improved our understanding of genes involved in the biosynthesis of cordycepin and other characteristic secondary metabolites in C. cicadae.

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RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection

Cited by 24 publications

References 52 publications

Comprehensive insights into host‐pathogen interaction between brown planthopper and a fungal entomopathogen by dual RNA sequencing

Comprehensive insights into host‐pathogen interaction between brown planthopper and a fungal entomopathogen by dual RNA sequencing

Immunotranscriptome analysis of Plutella xylostella reveals differences in innate immune responses to low‐ and high‐virulence Beauveria bassiana strain challenges

Identification of cordycepin biosynthesis-related genes through de novo transcriptome assembly and analysis in Cordyceps cicadae

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