RNA-Seq Analyses of the Role of miR-21 in Acute Pancreatitis

Li, Xun; Lin, Zhanwen; Wang, Lei; Liu, Qin; Cao, Zhi; Huang, Zhujuan; Zhong, Mingtian; Peng, Shuxian; Zhang, Yingheng; Li, Yong; Ma, Xiaodong

doi:10.1159/000495866

Cited by 21 publications

(20 citation statements)

References 32 publications

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“…Additionally, the overexpression of miR-21-3p in acinar cells inhibited apoptosis (Wang et al, 2018), which nullifies the protective effects of apoptosis for preventing leakage of necrotic substances and limiting inflammation (Najenson et al, 2018), and also promotes necroptosis of pancreatic acinar cells (Ma et al, 2015), thereby aggravating the injury of pancreatic tissues. Additionally, it was confirmed by several studies that deficiency of miR-21 in mice contributed to amelioration of pancreatic injury during AP (Ma et al, 2015;Li et al, 2018a). However, the upstream regulatory factors of miR-21 involved in SAP are not known yet.…”

Section: Discussionmentioning

confidence: 89%

“…A body of evidence indicates that altered expression of miRNAs is implicated in the pathogenesis of AP in animal models and human specimens (Xiang et al, 2017;Yang et al, 2019). Furthermore, functional studies also confirm that knockdown of certain miRNAs (e.g., miR-21) can be conducive to the reduction of damage caused by SAP (Ma et al, 2015;Li et al, 2018a). Although the roles of miRNAs have been well characterized, the precise mechanisms of miRNAs in SAP remain largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

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CircRNA Expression Profiles and the Potential Role of CircZFP644 in Mice With Severe Acute Pancreatitis via Sponging miR-21-3p

et al. 2020

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Severe acute pancreatitis (SAP) is the most serious type of pancreatitis with high morbidity and mortality. The underlying mechanism behind SAP pathogenesis is complex and remains elusive. Circular RNAs (circRNAs) are emerging as vital regulators of gene expression in various diseases by sponging microRNAs (miRNAs). However, the roles of circRNAs in the pathophysiology of SAP remain unknown. In the present study, next-generation RNA sequencing was utilized to identify circRNA transcripts in the pancreatic tissues from three SAP mice and three matched normal tissues. The differentially expressed circRNAs were confirmed by real-time PCR, and the biological functions of their interaction with miRNAs and mRNAs were analyzed. Our results demonstrate that 56 circRNAs were differentially expressed in SAP mice compared with normal controls. Six differentially expressed circRNAs were confirmed with the sequencing data. Importantly, we characterized a significantly downregulated circRNA derived from the ZFP664 gene in SAP. CircZFP644 was found to be negatively correlated with miR-21-3p, with a perfectly matched binding sequence to miR-21-3p. In conclusion, CircZFP644 may play an important role in the pathogenesis of SAP through sponging miR-21-3p. Our findings may provide novel insights regarding the workings of the pathophysiological mechanism of SAP and offer novel targets for SAP.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

CircRNA Expression Profiles and the Potential Role of CircZFP644 in Mice With Severe Acute Pancreatitis via Sponging miR-21-3p

et al. 2020

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“…It is confirmed that the decrease of HMGB1 in circulation can effectively alleviate the ALI caused by AP (Jo et al, ; Luan et al, ). The expression of HMGB1 in both immune cells and circulation is lower in AP mice with miR‐21 deficiency and the ALI is milder as well (Li et al, ). Supporting the data, miR‐21‐3p is upregulated in lung tissue of AP rats, which aggravates ALI and impairs lung oxygenation function (T. Wang et al, ).…”

Section: Mirnas and Ap Pathogenesismentioning

confidence: 99%

MicroRNAs in acute pancreatitis: From pathogenesis to novel diagnosis and therapy

Yang

Huang

Luo

et al. 2019

Journal Cellular Physiology

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Acute pancreatitis (AP) is an inflammatory disorder initiated by activation of pancreatic zymogens, leading to pancreatic injury and systemic inflammatory response. MicroRNAs (miRNAs) have emerged as important regulators of gene expression and key players in human physiological and pathological processes. Discoveries over the past decade have confirmed that altered expression of miRNAs is implicated in the pathogenesis of AP. Indeed, a number of miRNAs have been found to be dysregulated in various cell types involved in AP such as acinar cells, macrophages, and lymphocytes. These aberrant miRNAs can regulate acinar cell necrosis and apoptosis, local and systemic inflammatory response, thereby contributing to the initiation and progression of AP. Moreover, patients with AP possess unique miRNA signatures when compared with healthy individuals or those with other diseases. In view of their stability and easy detection, therefore, miRNAs have the potential to act as biomarkers for the diagnosis and assessment of patients with AP. In this review, we provide an overview of the novel cellular and molecular mechanisms underlying the roles of miRNAs during the disease processes of AP, as well as the potential diagnosis and therapeutic biomarkers of miRNAs in patients with AP.

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“…In recent years, a number of studies have reported that miRs are closely related to the occurrence and development of AP (11,27); however, the role of miRs in HTGAP has not been extensively reported. In the present study, RT-qPCR results suggested that the level of miR-372 in the serum of patients with HTGAP was significantly increased compared with patients who did not have HTGAP, indicating that the expression of miR-372 was abnormally high in patients with HTGAP.…”

Section: Discussionmentioning

confidence: 99%

Increased levels of miR‑372 correlate with disease progression in patients with hyperlipidemic acute pancreatitis

et al. 2020

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The aim of the present study was to investigate the expression of microRNA (miRNA)-372 in the serum of patients with hyperlipidemic acute pancreatitis (HTGAP), and its clinical significance. Patients with a serum lipid concentration ≥11.3 or 5.65-11.3 mmol/l with chylous serum were included in group A (n=40). The remaining patients did not have HTGAP and were included in group B (B). A further 25 patients with hyperlipidemia, but not AP (group C), and 30 healthy volunteers (group D) were recruited as controls. The level of miR-372 in the serum of group A (4.76±2.60) was significantly increased compared with groups B (0.98±0.80), C (0.85±0.62) and D (0.76±0.44); however, there was no significant difference in the expression of miR-372 between groups B, C and D. The expression level of miR-372 was significantly increased in the severe HTGAP group (6.45±2.20) compared with the mild HTGAP group (3.08±1.74). Further experiments suggested that the expression level of miR-372 was positively correlated with the level of triacylglycerol (r= 0.666; P<0.001) but not with the level of amylase (r=-0.145; P>0.05). ROC analysis indicated that the combined use of miR-372 expression levels and Acute Physiology and Chronic Health Evaluation II scoring improved the diagnostic value for HTGAP. In summary, the expression of miR-372 in HTGAP was significantly upregulated and increased with the severity of the disease. The results of the present study may provide a novel strategy for the diagnosis and severity assessment of HTGAP in the clinic.

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RNA-Seq Analyses of the Role of miR-21 in Acute Pancreatitis

Cited by 21 publications

References 32 publications

CircRNA Expression Profiles and the Potential Role of CircZFP644 in Mice With Severe Acute Pancreatitis via Sponging miR-21-3p

CircRNA Expression Profiles and the Potential Role of CircZFP644 in Mice With Severe Acute Pancreatitis via Sponging miR-21-3p

MicroRNAs in acute pancreatitis: From pathogenesis to novel diagnosis and therapy

Increased levels of miR‑372 correlate with disease progression in patients with hyperlipidemic acute pancreatitis

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