RNA-Seq analysis of duck embryo fibroblast cells gene expression during duck Tembusu virus infection

Pan, Yu; Wu, Xuedong; Cai, Wenjun; Cheng, Anchun; Wang, Mingshu; Chen, Shun; Huang, Juan; Yang, Qiao; Wu, Ying; Sun, Di; Mao, Sai; Zhu, Dekang; Liu, Mafeng; Zhao, Xinxin; Zhang, Shaqiu; Gao, Qun; Ou, Xumin; Tian, Bin; Yin, Zhongqiong; Jia, Renyong

doi:10.1186/s13567-022-01051-y

Cited by 4 publications

(3 citation statements)

References 54 publications

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“…The second-generation sequencing RNA-seq has been widely used in oncology, immunology, and cell biology in recent years ( 22 , 23 ). To clarify the differences in gene expression in the duck immune cells, we applied the RNA-seq transcriptome sequencing technology to analyze the gene expression differences in the duck monocytes/macrophages cell model infected with DPV CHv strain or CHa strain at 48 hpi.…”

Section: Resultsmentioning

confidence: 99%

Duck Plague Virus Negatively Regulates IFN Signaling to Promote Virus Proliferation via JNK Signaling Pathway

Tian²,

Wang³

et al. 2022

Front. Immunol.

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Duck plague virus (DPV), a member of the alphaherpesvirus subfamily, can cause severe damage and immunosuppression in ducks and geese in China. Since lacking an available cell model, the antiviral signal transduction pathways induction and regulation mechanisms related to DPV infection in duck cells are still enigmatic. Our previous study developed a monocyte/macrophages cell model, which has been applied to study innate immunity with DPV. In the present study, we compared and analyzed transcriptome associated with the DPV infection of CHv (virulent strain) and CHa (avirulent strain) at 48hpi based on the duck monocyte/macrophages cell model and RNA-seq technology. Differentially expressed genes (DEGs) analysis showed 2,909 and 2,438 genes altered in CHv and CHa infected cells compared with control cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the DEGs were mainly involved in biological processes such as metabolic pathways, viral infectious diseases, immune system, and signal transduction. The CHv and CHa virus differentially regulated MAPK, NF-κB, and IFN signaling pathways based on transcriptome sequencing data and RT-qPCR results. The JNK inhibitor SP600125 enhanced the IFN signaling, but potentially reduced the VSV and DPV titers in the cell culture supernatant, indicating that JNK negatively regulates the IFN pathway and the inflammatory pathway to promote virus proliferation. The research results may provide promising information to understand the pathogenesis of DPV and provide a novel mechanism by which DPV modulates antiviral signaling and facilitate virus proliferation through hijacking the JNK pathway, which provides a new means for the prevention and control of DPV infection.

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Section: Resultsmentioning

confidence: 99%

Duck Plague Virus Negatively Regulates IFN Signaling to Promote Virus Proliferation via JNK Signaling Pathway

Tian²,

Wang³

et al. 2022

Front. Immunol.

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“…Hence, renal tubular epithelial ( RTE ) cells are a promising option for GAstV-2 proliferation and pathogenesis analysis. RNA sequencing ( RNA-seq ), a powerful approach that can provide precise measurements for transcriptome profiling, can reveal dynamic changes in host gene expression during pathogen infections, including various viral infections ( Ma et al, 2021 ; Pan et al, 2022 ; Perlas et al, 2021 ). Therefore, in the current study, we isolated goose primary RTE ( GRTE ) cells from goose embryos and investigated GAstV-2 growth characteristics in GRTE cells and GAstV-2–GRTE cell interactions through RNA-seq.…”

Section: Introductionmentioning

confidence: 99%

Primary goose kidney tubular epithelial cells for goose astrovirus genotype 2 infection: establishment and RNA sequencing analysis

Guo,

Zhu,

et al. 2024

Poultry Science

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“…Its single open reading frame (ORF) encodes a unique polyprotein precursor, which is then cleaved into three structural proteins (C, prM, and E) and seven non-structural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) [ 15 , 16 ]. Previous studies have shown that DTMUV infection can effectively trigger type I IFN signaling and downstream ISG expression [ 17 , 18 ]. Avian type I IFNs and ISGs, such as VIPERIN, IFIT5, CMPK2, IRF7, IRF1, Mx, and OASL, play important roles in combating DTMUV infection [ 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Avian IRF1 and IRF7 Play Overlapping and Distinct Roles in Regulating IFN-Dependent and -Independent Antiviral Responses to Duck Tembusu Virus Infection

Xiang

Yang

Xiong

et al. 2022

Viruses

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Avian interferon regulatory factors 1 and 7 (IRF1 and IRF7) play important roles in the host’s innate immunity against viral infection. Our previous study revealed that duck tembusu virus (DTMUV) infection of chicken fibroblasts (DF1) and duck embryo fibroblasts (DEFs) induced the expression of a variety of IFN-stimulated genes (ISGs), including VIPERIN, IFIT5, CMPK2, IRF1, and IRF7. IRF1 was further shown to play a significant role in regulating the up-expression of VIPERIN, IFIT5, and CMPK2 and inhibiting DTMUV replication. In this study, we confirm, through overexpression and knockout approaches, that both IRF1 and IRF7 inhibit DTMUV replication, mainly via regulation of type I IFN expression, as well as the induction of IRF1, VIPERIN, IFIT5, CMPK2, and MX1. In addition, IRF1 directly promoted the expression of VIPERIN and CMPK2 in an IFN-independent manner when IRF7 and type I IFN signaling were undermined. We also found that non-structural protein 2B (NS2B) of DTMUV was able to inhibit the induction of IFN-β mRNA triggered by Newcastle disease virus (NDV) infection or poly(I:C) treatment, revealing a strategy employed by DTMUV to evade host’s immunosurveillance. This study demonstrates that avian IRF7 and IRF1 play distinct roles in the regulation of type I IFN response during DTMUV infection.

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RNA-Seq analysis of duck embryo fibroblast cells gene expression during duck Tembusu virus infection

Cited by 4 publications

References 54 publications

Duck Plague Virus Negatively Regulates IFN Signaling to Promote Virus Proliferation via JNK Signaling Pathway

Duck Plague Virus Negatively Regulates IFN Signaling to Promote Virus Proliferation via JNK Signaling Pathway

Primary goose kidney tubular epithelial cells for goose astrovirus genotype 2 infection: establishment and RNA sequencing analysis

Avian IRF1 and IRF7 Play Overlapping and Distinct Roles in Regulating IFN-Dependent and -Independent Antiviral Responses to Duck Tembusu Virus Infection

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