RNA-seq analysis of green tea polyphenols modulation of differently expressed genes in Enterococcus faecalis under low pH

Abstract

Significance and Impact of the Study: Enterococcus faecalis (E. faecalis) is a resident bacterium in the host, and can be used as probiotic additives. In this study, transcriptome technology was used to comprehensively analyse the mechanism of GTP improving the growth state of E. faecalis under low pH stress from the perspective of genes. This study showed that under low pH stress, a number of genes of E. faecalis associated with cell wall and membrane, transmembrane transport and metabolism process were significantly differentially expressed (P < 0Á05), while green tea polyphenols (GTP) intervention can partly relieve these negative influences. Significant changes were associated with selenocompound metabolism and aminoacyl-tRNA biosynthesis after the intervention of GTP (P < 0Á05). This will further deepen our understanding of the functional mechanism of GTP assisted E. faecalis in the gastrointestinal environment.

Antibacterial and Cytotoxic Silica-Polycaprolactone-Chlorogenic Acid Hybrids by Sol–Gel Route

“…Enterococcus faecalis, a Gram-positive bacterium, whose cell wall was constituted by the three components of peptidoglycan, teichoic acid, and polysaccharide [65], was inhibited mostly by SC15Py hybrids, highlighting that chlorogenic acid could be the main actor of the detected effect. In this context, chlorogenic acid was proven to exert antimicrobial activity by changing the permeability of microbial cell walls [66], and its bactericidal activity was against both Gram-positive and -negative bacteria [67,68].…”