RNA-Seq Analysis of the Key Long Noncoding RNAs and mRNAs Related to the Regulation of Acute Heat Stress in Rainbow Trout

Abstract

As the global climate warms, more creatures are threatened by high temperatures, especially cold-water fish such as rainbow trout. Evidence has demonstrated that long noncoding RNAs (lncRNAs) play a pivotal role in regulating heat stress in animals, but we have little understanding of this regulatory mechanism. The present study aimed to identify potential key lncRNAs involved in regulating acute heat stress in rainbow trout. lncRNA and mRNA expression profiles of rainbow trout head kidney were analyzed via high-throughput RNA sequencing, which exhibited that 1256 lncRNAs (802 up-regulation, 454 down-regulation) and 604 mRNAs (353 up-regulation, 251 down-regulation) were differentially expressed. These differentially expressed genes were confirmed to be primarily associated with immune regulation, apoptosis, and metabolic process signaling pathways through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and coding-noncoding co-expression network analysis. These results suggested that 18 key lncRNA-mRNA pairs are essential in regulating acute heat stress in rainbow trout. Overall, these analyses showed the effects of heat stress on various physiological functions in rainbow trout at the transcriptome level, providing a theoretical basis for improving the production and breeding of rainbow trout and the selection of new heat-resistant varieties.

Modelling the impact of replacing fish oil with plant oils: A meta‐analysis to match the optimal plant oil for major cultured fish

“…In our study, Atlantic salmon, European seabass, Gilthead seabream and Grouper were mostly oceanic species, but their reactive mode barely might explain the decrease in muscle lipid content at higher temperatures. 363 Also, many other researches have focused on the mechanism of Rainbow trout temperature stress, 364,365 and it has been proven that the degree Celsius of water is the primary determinant of its short-term survival. 366 Our research further confirmed that when fed with plant oils instead of fish oil, the mode of lipid accumulation of Rainbow trout largely depends on water temperature.…”

The Physiological Effects of Some Stress Indicators in Rainbow Trout Raised in Different Systems

“…Also in this sense, the idea was launched that long noncoding RNAs (lncRNAs) can be used in the selection of genetic variants of heat-resistant trout, given their essential role in the regulation of heat stress by association with genes involved in immune regulation, apoptosis and signaling pathways of metabolic activity [9].…”