RNA‐Seq Analysis of the Liver Transcriptome Reveals the Networks Regulating Treatment of Sitagliptin Phosphate plus Fuzhujiangtang Granule in the Zucker Diabetic Fatty Rats

Guo, Xuan; Sun, Wenjing; Xu, Guowang; Hou, Dan; Zhang, Zhuo; Wu, Lili; Liu, Tonghua

doi:10.1155/2020/8463858

Cited by 1 publication

(2 citation statements)

References 30 publications

(35 reference statements)

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“…The fold changes in the expression of 12 mRNAs are shown in Supplementary Table 1 . A previous study found that IRAK3 expression in liver tissue was related to diabetes development [ 24 ]. Therefore, we selected IRAK3 for further studies.…”

Section: Resultsmentioning

confidence: 99%

“…IRAK3 is an important negative regulator of TLR-mediated cell signaling, which can prevent the formation of IRAK–TNF receptor-associated factor-6 complexes and silence the NF-κB signaling pathway [ 35 ]. IRAK3, a key inhibitor of inflammation, promotes diabetes development [ 24 , 36 , 37 ]. Moreover, IRAK3 mRNA expression and protein levels were inhibited by PA treatment, and this effect was reversed by PSP treatment.…”

Section: Discussionmentioning

confidence: 99%

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Polygonatum sibiricum polysaccharides (PSP) improve the palmitic acid (PA)-induced inhibition of survival, inflammation, and glucose uptake in skeletal muscle cells

Cai

Zhu

et al. 2021

Bioengineered

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Polygonatum sibiricum polysaccharides (PSP) can decrease the levels of fasting blood glucose, total cholesterol, and triglyceride (TG) in hyperlipidemic and diabetic animals. It can also reduce inflammatory cytokines and promote glucose uptake in adipocytes. However, the underlying molecular mechanisms of PSP in improving insulin resistance (IR) in skeletal muscle remain unclear. In this study, palmitic acid (PA) induced an IR model in L6 myotubes. After treatment, cell proliferation was measured using the CCK8. miR-340-3p, glucose transporter 4 (GLUT-4), and interleukin-1 receptor-associated kinase 3 (IRAK3) expression was measured by qRT-PCR. IRAK3 protein levels were measured by Western blotting. Glucose in the cell supernatant, TG concentration in L6 myotubes, and the levels of IL-1β, IL-6, and TNF-α were measured by an ELISA. We found that cell survival, glucose uptake, and GLUT-4 expression in L6 myotubes were significantly suppressed, while lipid accumulation and inflammatory factor levels were enhanced by PA stimulation. Furthermore, PSP treatment markedly alleviated these effects. Interestingly, PSP also significantly reduced the upregulated expression of miR-340-3p in the L6 myotube model of IR. Furthermore, overexpression of miR-340-3p reversed the beneficial effects of PSP in the same IR model. miR-340-3p can bind to the 3′-untranslated regions of IRAK3 . Additionally, PA treatment inhibited IRAK3 expression, whereas PSP treatment enhanced IRAK3 expression in L6 myotubes. Additionally, miR-340-3p also inhibited IRAK3 expression in L6 myotubes. Taken together, PSP improved inflammation and glucose uptake in PA-treated L6 myotubes by regulating miR-340-3p/IRAK3, suggesting that PSP may be suitable as a novel therapeutic agent for IR.

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Section: Resultsmentioning

confidence: 99%