RNA-seq analysis of watermelon (Citrullus lanatus) to identify genes involved in fruit cracking

Jiang, Haikun; Tian, Hongmei; Yan, Chao‐Guo; Li, Jia; Wang, Yan; Wang, Mingxia; Jiang, Chang; Li, Yeyun; Jiang, Junguang; Fang, Ling; Zhang, Qian

doi:10.1016/j.scienta.2019.01.005

Cited by 30 publications

(26 citation statements)

References 17 publications

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“…In this study, eight auxin-related DEGs (Lc. 14.1626: ARF) were downregulated in the pericarp of cracking-susceptible cultivar 'Nuomici' when compared with that in cracking-resistant cultivar 'Huaizhi', which might lead to the retardation of pericarp growth and development in 'Nuomici'. In addition, four genes that encode the rate-limiting enzymes for ethylene biosynthesis (Lc.4.493: 1-aminocyclopropane -1 -carboxlic acid synthas, Lc.9.493: l-aminocycloProPane-1-carboxylic acid oxidas, Lc.9.503: l-aminocycloPro-Pane-1-carboxylic acid oxidas, Lc.9.506: l-aminocycloProPane-1-carboxylic acid oxidas) and two gibberellin-regulated genes (Lc.1.532 and Lc.1.534) were found to be downregulated in 'Nuomici' pericarp.…”

Section: Discussionmentioning

confidence: 98%

“…In sweet cherry, the expression level of genes related to cell wall remodeling and cuticular wax biosynthesis is higher in the pericarp of ‘Kordia’ than that in ‘Bing’, the difference between the expression of these genes may partially explain why ‘Kordia’ is more resistant to fruit cracking than ‘Bing’ [ 13 ]. Recently, RNA-Seq on both cracking-resistant and cracking-susceptible cultivars identified genes encoding XET1 , XET2 , glycerolipid triacylglycerol ( TAG ), and MADS box transcription factors ( MDP ) involved in fruit cracking in watermelon [ 14 ]. In addition, the decrease in the expression of PG and EXP via the RNA interference (RNAi) approach in tomato can promote generation of cuticular wax, protochitin, and cellulose in pericarp to increase its extensibility, therefore reducing the fruit cracking rate [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq Provides New Insights into the Molecular Events Involved in “Ball-Skin versus Bladder Effect” on Fruit Cracking in Litchi

Wang

Tang

et al. 2021

IJMS

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Fruit cracking is a disorder of fruit development in response to internal or external cues, which causes a loss in the economic value of fruit. Therefore, exploring the mechanism underlying fruit cracking is of great significance to increase the economic yield of fruit trees. However, the molecular mechanism underlying fruit cracking is still poorly understood. Litchi, as an important tropical and subtropical fruit crop, contributes significantly to the gross agricultural product in Southeast Asia. One important agricultural concern in the litchi industry is that some famous varieties with high economic value such as ‘Nuomici’ are susceptible to fruit cracking. Here, the cracking-susceptible cultivar ‘Nuomici’ and cracking-resistant cultivar ‘Huaizhi’ were selected, and the samples including pericarp and aril during fruit development and cracking were collected for RNA-Seq analysis. Based on weighted gene co-expression network analysis (WGCNA) and the “ball-skin versus bladder effect” theory (fruit cracking occurs upon the aril expanding pressure exceeds the pericarp strength), it was found that seven co-expression modules genes (1733 candidate genes) were closely associated with fruit cracking in ‘Nuomici’. Importantly, we propose that the low expression level of genes related to plant hormones (Auxin, Gibberellins, Ethylene), transcription factors, calcium transport and signaling, and lipid synthesis might decrease the mechanical strength of pericarp in ‘Nuomici’, while high expression level of genes associated with plant hormones (Auxin and abscisic acid), transcription factors, starch/sucrose metabolism, and sugar/water transport might increase the aril expanding pressure, thereby resulting in fruit cracking in ‘Nuomici’. In conclusion, our results provide comprehensive molecular events involved in the “ball-skin versus bladder effect” on fruit cracking in litchi.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

RNA-Seq Provides New Insights into the Molecular Events Involved in “Ball-Skin versus Bladder Effect” on Fruit Cracking in Litchi

Wang

Tang

et al. 2021

IJMS

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“…17,18 As one type of NGS, RNA-sequencing (RNA-Seq) can provide unprecedented opportunities for the generation of genomic information in uncharacterized earlier systems. 19,20 Recent literatures have also reported the transcriptome of many plants performed using RNA-Seq, such as tomato, 21 Rosa chinensis, 22 watermelon 23 as well as tobacco. 24 However, only a few publications focused on the investigations into yellow horn transcriptome.…”

Section: Introductionmentioning

confidence: 99%

Retracted Article: Investigation of yellow horn (Xanthoceras sorbifoliaBunge) transcriptome in response to different abiotic stresses: a comparative RNA-Seq study

Lang

Zheng

2020

RSC Adv.

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“…Hence, genome analyses including sequencing, developing molecular markers, transcriptome analyses etc. have gained importance (Sun et al, 2019;Jiang et al, 2019;Pandey et al, 2019). Transposons are also valuable sequences to figure out watermelon genome in detail.…”

Section: Resultsmentioning

confidence: 99%

Retrotransposon Analyses in Cucurbitaceae family

Marakli¹

2019

International Journal of Science Letters

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Retrotransposons are class I mobile elements, moving via an RNA intermediate. There are many retrotransposon-based molecular markers. Here, Nikita and Sukkula retrotransposons were investigated in Cucumis sativus L., Citrullus lanatus and Cucumis melo L. by using IRAP (Inter-Retrotransposon Amplified Polymorphism) molecular marker method. Nikita and Sukkula band profiles were similar in the plants, indicating only homomorphic band profiles. These barley-specific retrotransposons were identified in these three plant species for the first time. Findings could provide valuable information for understanding genomes of these plants and evolutionary relationships among them..

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RNA-seq analysis of watermelon (Citrullus lanatus) to identify genes involved in fruit cracking

Cited by 30 publications

References 17 publications

RNA-Seq Provides New Insights into the Molecular Events Involved in “Ball-Skin versus Bladder Effect” on Fruit Cracking in Litchi

RNA-Seq Provides New Insights into the Molecular Events Involved in “Ball-Skin versus Bladder Effect” on Fruit Cracking in Litchi

Retracted Article: Investigation of yellow horn (Xanthoceras sorbifoliaBunge) transcriptome in response to different abiotic stresses: a comparative RNA-Seq study

Retrotransposon Analyses in Cucurbitaceae family

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