RNA-seq and microRNA association analysis to explore the pathogenic mechanism of DHAV-1 infection with DEHs

Wang, Weiran; Li, Kun; Zhang, Tao; Dong, Hong; Liu, Jiaguo

doi:10.1007/s10142-023-01022-2

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…GO and KEGG analyses were performed using clusterProfiler to determine the biological processes and functional pathways of the DEGs and DEMs. Gene set enrichment analysis ( GSEA ) was performed for DEGs in vivo and in vitro during DHAV-1 infection according to our previous results ( Wang, et al, 2023 ). The GO and KEGG pathways with a revised significance threshold of P < 0.05.…”

Section: Methodsmentioning

confidence: 99%

mRNA and miRNA expression profiles reveal the potential roles of RLRs signaling pathway and mitophagy in duck hepatitis A virus type 1 infection

Wang,

Meng,

et al. 2024

Poultry Science

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Section: Methodsmentioning

confidence: 99%

mRNA and miRNA expression profiles reveal the potential roles of RLRs signaling pathway and mitophagy in duck hepatitis A virus type 1 infection

Wang,

Meng,

et al. 2024

Poultry Science

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Analysis of the regulatory role of miR-34a-5p/PLCD3 in the progression of osteoarthritis

Ying

Jiang

et al. 2023

Funct Integr Genomics

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Osteoarthritis is a heterogeneous disease with complex etiology. However, there is no effective treatment strategy at present. The purpose of this study is to explore the miRNA-mRNA regulatory network and molecular mechanism that regulate the progression of osteoarthritis. In this article, we downloaded dataset (GSE55457, GSE82107, GSE143514 and GSE55235) from Gene Expression Omnibus (GEO) to screen differentially expressed mRNAs in osteoarthritis. Then, through weighted gene co-expression network analysis (WGCNA), functional enrichment, protein-protein interaction networks (PPI), miRNA-mRNA co-expression network, ROC curve, immune in ltration analysis and qPCR, the mRNA PLCD3, which was highly expressed in osteoarthritis and had clinical predictive value, was screened. Whereafter, we found that PLCD3 directly targets miR-34a-5p through DIANA and dual luciferase experiment. And the expression of PLCD3 and miR-34a-5p were negatively correlated. In addition, CCK-8 and wound healing showed that miR-34a-5p mimic inhibited hFLS-OA cell proliferation and promoted hFLS-OA cell migration.PLCD3 overexpression showed an opposite trend. Western further found that overexpression of miR-34a-5p reduced the protein expression levels of p-PI3K and p-AKT, while overexpression of PLCD3 also showed the opposite trend. Besides, combined with the effect of PI3K/AKT pathway inhibitor BIO(IC50 = 5.95 µM), the results showed that overexpression of miR-34a-5p increased the inhibitory effect of BIO on p-PI3K and p-AKT protein expression, while overexpression of PLCD3 signi cantly reversed the inhibitory effect. In all, the miR-34a-5p/PLCD3 axis may mediate the PI3K/AKT pathway in regulating cartilage homeostasis in synovial osteoarthritis. These data indicate that miR-34a-5p/PLCD3 may be a new prognostic factor in the pathology of synovial osteoarthritis.

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Advances in the Duck Hepatitis A virus and lessons learned from those in recent years

Zhao,

Wu,

Wang

et al. 2024

Microbial Pathogenesis

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RNA-seq and microRNA association analysis to explore the pathogenic mechanism of DHAV-1 infection with DEHs

Cited by 3 publications

References 41 publications

mRNA and miRNA expression profiles reveal the potential roles of RLRs signaling pathway and mitophagy in duck hepatitis A virus type 1 infection

mRNA and miRNA expression profiles reveal the potential roles of RLRs signaling pathway and mitophagy in duck hepatitis A virus type 1 infection

Analysis of the regulatory role of miR-34a-5p/PLCD3 in the progression of osteoarthritis

Advances in the Duck Hepatitis A virus and lessons learned from those in recent years

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