RNA-seq approach to analysis of gene expression profiles in dark green islands and light green tissues of Cucumber mosaic virus-infected Nicotiana tabacum

Chen, Lijuan; Fei, Chunyan; Zhu, Lin; Xu, Zhe; Zou, Wenshan; Yang, Ting; Lin, Honghui; Xi, Dehui

doi:10.1371/journal.pone.0175391

Cited by 8 publications

(5 citation statements)

References 57 publications

(88 reference statements)

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“…Infection by plant viruses often induces the formation of virus-free dark green islands (DGIs) surrounded by virus-present light green tissues (LGTs) on infected leaves. In Cucumber mosaic virus (CMV)-infected N. tabacum plants, the expression levels of genes encoding expansins in LGTs are lower than those in DGIs (Chen et al, 2017). Infections by Rice dwarf virus (RDV), Rice stripe virus (RSV) and Rice tungro spherical virus (RTSV) also suppress various cell wall-related genes such as cellulose synthase, extensin and expansin, leading to the stunting phenotype (Shibata et al, 2007;Satoh et al, 2010Satoh et al, , 2011Budot et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

Park

Renaud

et al. 2017

The Plant Journal

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Plasmodesmata (PD), unique to the plant kingdom, are structurally complex microchannels that cross the cell wall to establish symplastic communication between neighbouring cells. Viral intercellular movement occurs through PD. To better understand the involvement of PD in viral infection, we conducted a quantitative proteomic study on the PD-enriched fraction from Nicotiana benthamiana leaves in response to infection by Turnip mosaic virus (TuMV). We report the identification of a total of 1070 PD protein candidates, of which 100 (≥2-fold increase) and 48 (≥2-fold reduction) are significantly differentially accumulated in the PD-enriched fraction, when compared with protein levels in the corresponding healthy control. Among the differentially accumulated PD protein candidates, we show that an α-expansin designated NbEXPA1, a cell wall loosening protein, is PD-specific. TuMV infection downregulates NbEXPA1 mRNA expression and protein accumulation. We further demonstrate that NbEXPA1 is recruited to the viral replication complex via the interaction with NIb, the only RNA-dependent RNA polymerase of TuMV. Silencing of NbEXPA1 inhibits plant growth and TuMV infection, whereas overexpression of NbEXPA1 promotes viral replication and intercellular movement. These data suggest that NbEXPA1 is a host factor for potyviral infection. This study not only generates a PD-proteome dataset that is useful in future studies to expound PD biology and PD-mediated virus-host interactions but also characterizes NbEXPA1 as the first PD-specific cell wall loosening protein and its essential role in potyviral infection.

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Section: Discussionmentioning

confidence: 99%

NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

Park

Renaud

et al. 2017

The Plant Journal

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“…1 a) indicating a correlation of CMV infection with the induction of NbAO in N. benthamiana . CMV is inducing a transcriptional reprogramming of the host plant (Chen et al 2017 ). The arising question is whether the NbAO induction is part of a defense response of the host plant to CMV?…”

Section: Resultsmentioning

confidence: 99%

Silencing of a Nicotiana benthamiana ascorbate oxidase gene reveals its involvement in resistance against cucumber mosaic virus

Ahmed,

Kaldis,

Voloudakis

2024

Planta

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Main conclusion Silencing of an ascorbate oxidase (AO) gene in N. benthamiana enhanced disease severity from cucumber mosaic virus (CMV), showing higher accumulation and expansion of the spreading area of CMV. Abstract A Nicotiana benthamiana ascorbate oxidase (NbAO) gene was found to be induced upon cucumber mosaic virus (CMV) infection. Virus-induced gene silencing (VIGS) was employed to elucidate the function of AO in N. benthamiana. The tobacco rattle virus (TRV)-mediated VIGS resulted in an efficient silencing of the NbAO gene, i.e., 97.5% and 78.8% in relative quantification as compared to the control groups (TRV::eGFP- and the mock-inoculated plants), respectively. In addition, AO enzymatic activity decreased in the TRV::NtAO-silenced plants as compared to control. TRV::NtAO-mediated NbAO silencing induced a greater reduction in plant height by 15.2% upon CMV infection. CMV titer at 3 dpi was increased in the systemic leaves of NbAO-silenced plants (a 35-fold change difference as compared to the TRV::eGFP-treated group). Interestingly, CMV and TRV titers vary in different parts of systemically infected N. benthamiana leaves. In TRV::eGFP-treated plants, CMV accumulated only at the top half of the leaf, whereas the bottom half of the leaf was “occupied” by TRV. In contrast, in the NbAO-silenced plants, CMV accumulated in both the top and the bottom half of the leaf, suggesting that the silencing of the NbAO gene resulted in the expansion of the spreading area of CMV. Our data suggest that the AO gene might function as a resistant factor against CMV infection in N. benthamiana.

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“…CMV, as a systemically infectious virus, causes economic losses worldwide 3 . To identify genes involved in broad-spectrum tolerance to CMV, three previous studies investigated tobacco plant responses to CMV and the mechanisms underlying disease development using next-generation sequencing to monitor transcriptional changes in systemically infected leaves, with the results that thousands of DEGs were discovered 28,38,39 . In this study, we used RNA-Seq approaches to analyze gene expression changes in CMV-inoculated leaves between two cultivars.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of two cultivars of tobacco in response to Cucumber mosaic virus infection

Liu

Zhao

Cheng

et al. 2019

Sci Rep

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Cucumber mosaic virus (CMV) is among the most important plant virus infections, inducing a variety of disease symptoms. However, the molecular mechanisms underlying plant responses to CMV infection remain poorly understood. In this study, we performed RNA sequencing analysis of tolerant (Taiyan8) and susceptible (NC82) tobacco cultivars on CMV-infected plants, using mock-inoculated plants as a control. The propagation of CMV in inoculated leaves did not show obvious difference between two cultivars, whereas virus accumulation in systemic leaves of Taiyan8 was smaller than those of NC82 at the same time point. We observed 765 and 1,011 differentially expressed genes (DEGs) in Taiyan8 and NC82, respectively, in CMV-inoculated leaves. DEGs related to reactive oxygen species, salicylic acid signal transduction, and plant–pathogen interaction were upregulated or downregulated in Taiyan8, which indicates that defense response pathways to CMV were activated in the tolerant cultivar. In addition, we identified several DEGs related to disease defense and stress resistance showing opposing expression patterns in the two cultivars. Our comparative transcriptome analysis will improve our understanding of the mechanisms of CMV tolerance in plants, and will be of great importance in the molecular breeding of CMV- tolerant genotypes.

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RNA-seq approach to analysis of gene expression profiles in dark green islands and light green tissues of Cucumber mosaic virus-infected Nicotiana tabacum

Cited by 8 publications

References 57 publications

NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

Silencing of a Nicotiana benthamiana ascorbate oxidase gene reveals its involvement in resistance against cucumber mosaic virus

Transcriptome analysis of two cultivars of tobacco in response to Cucumber mosaic virus infection

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