RNA-Seq based global transcriptome analysis of rice unravels the key players associated with brown planthopper resistance

Vanisri, S.; Kudapa, H.; Muthuramalingam, Pandiyan; Nadimpalli, Rama Gopal Varma; Vattikuti, Jhansi Lakshmi; Anjali, C.; Satish, Lakkakula; Ramesh, Manikandan; Mulinti, Sreedhar

doi:10.1016/j.ijbiomac.2021.09.058

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…In addition, 24 genes associated with the biosynthetic pathways of volatile terpenoids and HPL-derived green leaf volatiles were upregulated after S. litura infestation, highlighting their potential roles in S. litura-induced pear defenses. Unlike defense-related nonvolatile and volatile secondary metabolites, growth-related photosynthesis was suppressed in S. litura-infested P. betuleafolia (Table S7), which is similar to the findings reported in the defense response of potato (Mao et al, 2022), cotton (Zhang et al, 2022), rice (Wang et al, 2020;Satturu et al, 2021), wheat (Zhang et al, 2020) and tomato (Ke et al, 2021) plants induced by herbivores. In herbivoredamaged plants, suppression of photosynthesis might be a Behavioral preference of Spodoptera litura larvae for (E)-b-ocimene.…”

Section: Discussionsupporting

confidence: 87%

Functional characterization of a terpene synthase responsible for (E)-β-ocimene biosynthesis identified in Pyrus betuleafolia transcriptome after herbivory

Huang

Zhang

et al. 2022

Front. Plant Sci.

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(E)-β-ocimene, a ubiquitous monoterpene volatile in plants, is emitted from flowers to attract pollinators and/or from vegetative tissues as part of inducible defenses mediated by complex signaling networks when plants are attacked by insect herbivores. Wild pear species Pyrus betuleafolia used worldwide as rootstock generally displays valuable pest-resistant traits and is a promising genetic resource for pear breeding. In the current study, transcriptional changes in this wild pear species infested with a polyphagous herbivore Spodoptera litura and the underlying molecular mechanisms were fully investigated. A total of 3,118 differentially expressed genes (DEGs) were identified in damaged pear leaf samples. Spodoptera litura larvae infestation activated complex phytohormonal signaling networks in which jasmonic acid, ethylene, brassinosteroids, cytokinin, gibberellic acid and auxin pathways were induced, whereas salicylic acid and abscisic acid pathways were suppressed. All DEGs associated with growth-related photosynthesis were significantly downregulated, whereas most DEGs involved in defense-related early signaling events, transcription factors, green leaf volatiles and volatile terpenes were significantly upregulated. The PbeOCS (GWHGAAYT028729), a putative (E)-β-ocimene synthase gene, was newly identified in P. betuleafolia transcriptome. The upregulation of PbeOCS in S. litura-infested pear leaves supports a potential role for PbeOCS in herbivore-induced plant defenses. In enzyme-catalyzed reaction, recombinant PbeOCS utilized only geranyl pyrophosphate but not neryl diphosphate, farnesyl pyrophosphate or geranylgeranyl diphosphate as a substrate, producing (E)-β-ocimene as the major product and a trace amount of (Z)-β-ocimene. Moreover, as a catalytic product of PbeOCS, (E)-β-ocimene showed repellent effects on larvae of S. litura in dual-choice bioassays. What is more, (E)-β-ocimene increased mortalities of larvae in no-choice bioassays. These findings provide an overview of transcriptomic changes in wild pears in response to chewing herbivores and insights into (E)-β-ocimene biosynthesis in pear plants, which will help elucidate the molecular mechanisms underlying pear-insect interactions.

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Section: Discussionsupporting

confidence: 87%

Functional characterization of a terpene synthase responsible for (E)-β-ocimene biosynthesis identified in Pyrus betuleafolia transcriptome after herbivory

Huang

Zhang

et al. 2022

Front. Plant Sci.

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Combined strategy employing MutMap and RNA-seq reveals genomic regions and genes associated with complete panicle exsertion in rice

Hake,

Ballichatla,

Barbadikar

et al. 2023

Mol Breeding

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A time-course transcriptomic analysis reveals the key responses of a resistant rice cultivar to brown planthopper infestation

Dong,

Wu,

Lian

et al. 2024

Sci Rep

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The brown planthopper (BPH) is one of the most problematic pests affecting rice ( Oryza sativa L.) yields in Asia. Breeding rice varieties containing resistance genes is the most economical and effective means of controlling BPH. In this study, the key factors in resistance to BPH were investigated between the high-resistance rice variety “R26” and the susceptible variety “TN1” using RNA-sequencing. We identified 9527 differentially expressed genes (DEGs) between the rice varieties under BPH-induced stress. Weighted time-course gene co-expression network analysis (WGCNA) indicated that the increased expression of genes is associated with plant hormones, MAPK signaling pathway and biosynthesis of other secondary metabolites, which were involved in disease resistance. A connection network identified a hub gene, OsREM4.1 (BGIOSGA024059), that may affect rice resistance to the BPH. Knocking out OsREM4.1 in rice can lead to a decrease in callose, making it less resistant to BPH. Overall, the expression of differentially expressed genes varies among rice varieties with different resistance in BPH invasion. Inaddition, R26 enhances resistance to BPH by upregulating genes and secondary metabolites related to stress resistance and plant immunity. In summary, our study provides valuable insights into the genome-wide expression profile of DEGs in rice under BPH invasion through high-throughput sequencing, and further suggests that R26 can be used to develop high resistance rice lines in BPH resistant breeding programs. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-73546-x.

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RNA-Seq based global transcriptome analysis of rice unravels the key players associated with brown planthopper resistance

Cited by 3 publications

References 31 publications

Functional characterization of a terpene synthase responsible for (E)-β-ocimene biosynthesis identified in Pyrus betuleafolia transcriptome after herbivory

Functional characterization of a terpene synthase responsible for (E)-β-ocimene biosynthesis identified in Pyrus betuleafolia transcriptome after herbivory

Combined strategy employing MutMap and RNA-seq reveals genomic regions and genes associated with complete panicle exsertion in rice

A time-course transcriptomic analysis reveals the key responses of a resistant rice cultivar to brown planthopper infestation

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