RNA-Seq-based transcriptome analysis of stem development and dwarfing regulation in Agapanthus praecox ssp. orientalis (Leighton) Leighton

Zhang, Di; Ren, Li; Yue, Jianhua; Shi, Yubo; LiHuan, Zhuo; Wang, Sheng; Shen, Xiaohui

doi:10.1016/j.gene.2015.04.013

Cited by 22 publications

(17 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the down-regulation of auxin signaling genes such as auxin receptor TIR1, auxin response factor ARF, and AUX/IAA transcriptional repressors indicated that auxin signal transduction was somehow blocked in cotton internodes by MC. Similarly, the inhibited expression of auxin-related genes were also observed in Agapanthus praecox (Zhang et al, 2015) and Jatropha curcas (Seesangboon et al, 2018), and in litchi by uniconazole (Ohnishi et al, 2006). Opposite to the downregulation by MC, we found that exogenous GA 3 upregulated the expression of TAR2 and PIN6 (Figure 8), similar to previous studies in Populus (Björklund et al, 2007), Arabidopsis (Li et al, 2014), and Eucalyptus grandis .…”

Section: Remarkably Altered the Biosynthesis And Signaling Transdusupporting

confidence: 89%

“…ETR2 (ethylene receptor) and EBF1 (ethylene signaling) were inhibited by MC, too. Zhang et al (2015) reported that PAC repressed the expression of ethylene signal transduction genes in the dwarfing scape of Agapanthus praecox. Exogenous GA 3 increased the expression of ETR, but did not affect ACOH (Figure 8), consistent with a previous report that the expression of ethylene-related genes were altered differently by exogenous GA (Di et al, 2019).…”

Section: Remarkably Altered the Biosynthesis And Signaling Transdumentioning

confidence: 99%

“…Recent studies have shown that plant growth retardants could affect the expression of hormonerelated genes. PAC affected the expression of many hormonerelated genes in Jatropha (Seesangboon et al, 2018) and Agapanthus praecox (Zhang et al, 2015). The expression of genes related to GA, CK, and ABA was altered by uniconazole in duckweed (Liu et al, 2015).…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Transcriptome Profiling of the Elongating Internode of Cotton (Gossypium hirsutum L.) Seedlings in Response to Mepiquat Chloride

et al. 2020

View full text Add to dashboard Cite

The plant growth retardant mepiquat chloride (MC) has been extensively used to produce compact plant canopies and increase yield in cotton (Gossypium hirsutum L.). Previous studies showed that MC reduced plant height and internode length by inhibiting GA biosynthesis and cell elongation. However, whether there are other molecular mechanisms underlying MC-induced growth retardation has remained largely unknown. In the present study, we conducted histological, transcriptomic, and phytohormone analyses of the second elongating internodes of cotton seedlings treated with MC. Histological analysis revealed that the MC shortened the internodes through suppressing both cell division and cell elongation. Consistent with the observed phenotype, many genes related to cell growth were significantly downregulated by MC. Transcriptome profiling showed that the expression of genes related not only to GA, but also to auxin, brassinosteroid (BR), and ethylene metabolism and signaling was remarkably suppressed, whereas that of genes related to cytokinin (CK) and abscisic acid (ABA) metabolism was induced by MC. Consistent with the expression pattern, significant decrease of endogenous GA, auxin, and BR content, but an increase in CK content was observed after MC treatment. Most of these hormone related genes displayed opposite regulation pattern by exogenous GA 3 treatment compared to MC; simultaneous application of MC and GA 3 could alleviate the genes expression changes induced by MC treatment, indicating MC does not directly affect other plant hormones, but through the inhibition of the GA biosynthesis. In addition, the expression of genes related to secondary metabolism and many transcription factors (TFs) were differentially regulated by MC. In summary, we confirmed the important role of GA in MC-induced growth inhibition of cotton, and further found that other hormones were also involved in this process in a GA-dependent manner. This study provides novel insights into the molecular mechanism underlying the MC-mediated inhibition of internode elongation in cotton seedlings.

show abstract

Section: Remarkably Altered the Biosynthesis And Signaling Transdusupporting

confidence: 89%

Section: Remarkably Altered the Biosynthesis And Signaling Transdumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Transcriptome Profiling of the Elongating Internode of Cotton (Gossypium hirsutum L.) Seedlings in Response to Mepiquat Chloride

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Meanwhile, DPC may suppressed hormone expression. In Agapanthus praecox, auxin-related genes could be inhibited by DPC treatment [49]. Surprisingly, the present study also indicated that DPC increased several hormonal genes.…”

Section: Discussioncontrasting

confidence: 42%

Global Transcriptome Changes of Elongating Internode of Sugarcane in Response to Mepiquat Chloride

Chen

Fan

Zhou

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Mepiquat chloride (DPC) is an extensively used chemical to control internode growth and compact canopies in cultured plants. Previous studies suggested that DPC could inhibit gibberellin biosynthesis in sugarcane. Unfortunately, the molecular mechanism underlying the suppressive effects of DPC on plant growth is still largely unknown. Results: In the present study, we first obtained the high-quality long transcripts from internode of sugarcane by PacBio Sequel System. A total of 72,671 isoforms with N50 as 3,073 were generated. These long isoforms were used for the following RNA-seq as reference. Subsequently, short reads generated from Illumina Hiseq 4000 platform were used for comparing the differentially expressed genes in DPC and control groups. The transcriptome profiling showed the 6 days post DPC treatment had the most significant changed genes. These genes were related to plant hormone signal transduction and biosynthesis of several metabolites, indicating DPC affected multiple pathways beside depressed gibberellin biosynthesis. The network of DPC on the key stage was illustrated by weighted gene co-expression network analysis (WGCNA). Among the constructed 36 modules, the top positive correlated module with stage of 6 day post spraying DPC was sienna3. Stf0 sulphotransferase, cyclin-like F-box and HOX12 were the hub genes in sienna3 that had high correlation with other genes in this module. The qPCR demonstrated the high accuracy of RNA-seq result. Conclusion: Taken together, we demonstrated the key role of these genes in DPC-induced growth inhibition in sugarcane.

show abstract

“…Transcription factors (TFs) are the most important regulatory proteins that activate or inhibit transcription of multiple genes that orchestrate specific physiological processes during plant development and in response to environmental changes. Many TFs have been reported to regulate plant development and dwarfing, including WRKY, AP2/ EREBP, bHLH, C2H2 and ARF; of these, most is known about the WRKY family of TFs (Ou et al, 2015;Zhang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

et al. 2017

View full text Add to dashboard Cite

Dwarfing rootstocks enable high-density planting and are therefore highly desirable in modern apple (Malus domestica) production. M26 is a semi-dwarfing rootstock that is used worldwide, but identifying intensive dwarfing rootstock is a major goal of apple breeding programs. Herein, we show that MdWRKY9 mediates dwarfing by directly inhibiting the transcription of the brassinosteroid (BR) rate-limiting synthetase MdDWF4 and reducing BR production. We found that the transcriptional factor MdWRKY9 is highly expressed in all tested dwarfing rootstocks. Transgenic lines of M26 rootstock overexpressing MdWRKY9 exhibit further dwarfing, which resulted from the reduced BR levels and was reversed via exogenous brassinolide treatment. Both an in vivo chromatin immunoprecipitation (ChIP) analysis and an in vitro electrophoretic mobility shift assay (EMSA) indicated that MdWRKY9 binds to the promoter of MdDWF4. Furthermore, MdWRKY9 repressed MdDWF4 expression in stable transgenic apple plants as determined by quantitative PCR. In addition, RNA-interfered expression of MdWRKY9 in transiently transformed apple calli led to a significant increase of MdDWF4, suggesting MdWRKY9 plays a critical role in regulating the expression of MdDWF4. We report a novel dwarfing mechanism in perennial woody plants that involves WRKY-controlled BR production, and present a new dwarfing M26 rootstock for potential applications in apple production.

show abstract

RNA-Seq-based transcriptome analysis of stem development and dwarfing regulation in Agapanthus praecox ssp. orientalis (Leighton) Leighton

Cited by 22 publications

References 93 publications

Transcriptome Profiling of the Elongating Internode of Cotton (Gossypium hirsutum L.) Seedlings in Response to Mepiquat Chloride

Transcriptome Profiling of the Elongating Internode of Cotton (Gossypium hirsutum L.) Seedlings in Response to Mepiquat Chloride

Global Transcriptome Changes of Elongating Internode of Sugarcane in Response to Mepiquat Chloride

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

Contact Info

Product

Resources

About