RNA-Seq comparative analysis reveals the response of Enterococcus faecalis TV4 under fluoride exposure

Li, Guannan; Shi, Min; Zhao, Shan; Li, Dan; Long, Yang; Zhu, Yong

doi:10.1016/j.gene.2019.144197

Cited by 16 publications

(12 citation statements)

References 78 publications

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“…The Enterococcus genus was reduced in midgut of gypsy moth reared on antibiotic mixtures (penicillin, gentamicin, rifampicin, and streptomycin), which was necessary for B. thuringiensis -induced larval mortality ( Broderick et al, 2006 ). In Bombyx mori , inhibition of Enterococcus by fluoride can increase amino acid synthesis, reduce cell carbohydrate transport and energy production ( Li G. et al, 2020 ). Our data suggested that the abundance of Enterococcus was significantly reduced in the antibiotics-treated group, and the transcriptome data suggested that genes related to carbohydrate transport and metabolism and energy production were also upregulated, implying that the Enterococcus genus may affect the metabolism level in the gut of S. frugiperda .…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

et al. 2021

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Insect gut microbiota plays important roles in acquiring nutrition, preventing pathogens infection, modulating immune responses, and communicating with environment. Gut microbiota can be affected by external factors such as foods and antibiotics. Spodoptera frugiperda (Lepidoptera: Noctuidae) is an important destructive pest of grain crops worldwide. The function of gut microbiota in S. frugiperda remains to be investigated. In this study, we fed S. frugiperda larvae with artificial diet with antibiotic mixture (penicillin, gentamicin, rifampicin, and streptomycin) to perturb gut microbiota, and then examined the effect of gut microbiota dysbiosis on S. frugiperda gene expression by RNA sequencing. Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria were the most dominant phyla in S. frugiperda. We found that the composition and diversity of gut bacterial community were changed in S. frugiperda after antibiotics treatment. Firmicutes was decreased, and abundance of Enterococcus and Weissella genera was dramatically reduced. Transcriptome analysis showed that 1,394 differentially expressed transcripts (DETs) were found between the control and antibiotics-treated group. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that antibiotics-induced dysbiosis affected many biological processes, such as energy production, metabolism, and the autophagy–lysosome signal pathway. Our results indicated that dysbiosis of gut microbiota by antibiotics exposure affects energy and metabolic homeostasis in S. frugiperda, which help better understand the role of gut microbiota in insects.

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Section: Discussionmentioning

confidence: 99%

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

et al. 2021

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“…amylase and cellulase) to utilize plant polysaccharides (Anand et al ., 2010). For instance, symbiotic enterococci of silkworm secrete extracellular digestive enzymes and possess the phosphotransferase system, which can efficiently participate in the carbohydrate transport and metabolism (Li et al ., 2020a; Li et al ., 2020b). Moreover, silkworm relies on intestinal symbionts for cellulose digestion due to loss of cellulose‐encoding genes among lepidopteran species; thus, cellulase is critical for food digestion in the digestive tract (Paniagua et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…A recent study has proven that the intestinal microbiota affects host phospholipid metabolism by modifying a number of phosphatidylcholine species (Velagapudi et al ., 2010). In addition, Enterococcus , Staphylococcus , Brevibacterium , Corynebacterium and Brevundimonas can secrete lipases to catabolize the lipids of mulberry leaves to fatty acids (Feng et al ., 2011; Li et al ., 2020a; Li et al ., 2020b). Concurrently, fatty acids from diets can be used to generate glycerophospholipids to participate in the phospholipid metabolism of organisms (Frisca et al ., 2012).…”

Section: Discussionmentioning

confidence: 99%

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Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

Zheng

Zhu

et al. 2022

Environmental Microbiology

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The symbiotic association between bacterial symbionts and insect hosts is a complicated process that is not completely understood. Herein, we used a silkworm model to study the association between symbiotic Bacillus and lepidopteran insect by investigating the changes in intestinal microbiota and hemolymph circulating metabolites of silkworm after symbiotic Bacillus subtilis treatment. Results showed that B. subtilis can generate a variety of primary and secondary metabolites, such as B vitamins and antimicrobial compounds, to provide micronutrients and enhance the pathogen resistance of their insect host. Shifts in the relative abundance of Enterococcus, Brevibacterium, Buttiauxella, Pseudomonas, Brevundimonas and Limnobacter had significant correlations with the concentrations of differential metabolites (e.g. phospholipids and certain amino acids) in insect hemolymph. The antimicrobial compounds secreted by B. subtilis were the primary driving force for the reconstruction of intestinal microbiota. Meanwhile, the altered levels of circulating metabolites in multiple metabolic pathways were potential adaptive mechanism of insect hosts in response to the shifts of intestinal microbiota. Our findings provided concrete evidence that bacterial intestinal symbiont can alter the physiological state of insects and highlighted the importance of the compositional alterations of intestinal microbiota as a source of variation in circulating metabolites of insect hosts.

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“…It has been widely used in functional gene research [10,11], metabolic pathway and mechanism analysis [12], and key marker screening [13,14]. There have been reports on the transcriptome [15][16][17], proteome [9], and microbial diversity [5] after NaF stress in silkworm. However, the differences in metabolic pathways of fluoride-tolerant and sensitive silkworm varieties under NaF stress have not been reported using metabonomics.…”

Section: Introductionmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in Bombyx mori

Zhang

Qian

et al. 2019

Insects

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Fluoride tolerance is an important economic trait in sericulture, especially in some industrial development regions. Analyses of physiological changes involving structural damage to the insect body and molecular analyses of some related genes have focused on this area; however, the changes that occur at the metabolic level of silkworms after eating fluoride-contaminated mulberry leaves remain unclear. Here, metabonomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) to analyze the changes in midgut tissue after NaF stress using silkworm strains 733xin (susceptible stain) and T6 (strain resistant to fluoride), which were previously reported by our laboratory. Differential metabolomics analysis showed that both T6 and 733xin strains displayed complex responses after exposure to 200 mg/kg NaF. The purine metabolism and arginine and proline metabolic pathways of fluoride-tolerant strains reached significant levels, among which 3′-adenylic acid and hypoxanthine were significantly upregulated, whereas guanine, allantoic acid, xanthine, N-acetyl-L-glutamic acid, and pyruvate were significantly downregulated. These metabolic pathways may be related to the fluoride tolerance mechanism of NaF poisoning and tolerant strains.

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RNA-Seq comparative analysis reveals the response of Enterococcus faecalis TV4 under fluoride exposure

Cited by 16 publications

References 78 publications

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

Gut Microbiota Dysbiosis Influences Metabolic Homeostasis in Spodoptera frugiperda

Bacillus symbiont drives alterations in intestinal microbiota and circulating metabolites of lepidopteran host

Gas Chromatography-Mass Spectrometry Based Midgut Metabolomics Reveals the Metabolic Perturbations under NaF Stress in Bombyx mori

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