RNA-seq data of tea mosquito bugs, Helopeltis bradyi, antennae

Abstract

Tea Mosquito Bug (TMB), Helopeltis bradyi (Hemiptera: Miridae) is one of the major pest infesting tea and cocoa plantations worldwide. Developing olfaction-based control methods was urges as an alternative to commonly used but non-environmental friendly chemical pesticides. However, the molecular mechanisms underlying TMB reception mechanism are still lacking. Here, we collected a pooled male and female TMB antennae for RNA extraction followed by sequencing using the BGISEQ-500 platform and de novo assembly. TMB antennae RNA-seq data yielded 32,142 unigenes with N50 and GC (%) were 2322 and 40.25; subsequently. The RNA-seq data are available in GenBank Sequence Read Archive (SRA) database with accession number SRR13327229. De novo transcriptome analysis had identified several genes involved in TMB odorant reception includes; 39 OBPs (odorant binding proteins), 10 CSPs (chemosensory proteins), 81 Ors (odorant receptors), 1 Orcos (co-receptors), 9 SNMPs (sensory neuron membrane proteins), 3 GRs (gustatory receptors) and 4 IRs (ionotropic receptors). Our study presents the first RNA seq for TMB antennae, which serve the primary molecular resources data, which will facilitate further research to develop olfaction-based control methods, potentially contribute to TMB management strategies.

Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance

“…Then the mRNA was isolated from total RNA with the Dynabeads mRNA Purification Kit (Invitrogen) and fragmented to 200–400 bp using a fragmentation buffer (Ambion, #AM8740). The cDNA library was generated from the purified mRNA following the manufacturer’s guideline of Optimal Dual-mode mRNA Library Prep Kit (BGI, Shenzhen, China) as previously described [ 68 ], and paired-end 150 bp (PE150) sequencing was perform on a BGISEQ-500 platform (BGI).…”