RNA-seq Provides Novel Insights into Response to Acute Salinity Stress in Oriental River Prawn Macrobrachium nipponense

Abstract

The oriental river prawn Macrobrachium nipponense is an important aquaculture species in China, Vietnam, and Japan. This species could survive in the salinity ranging from 7 to 20 ppt and accelerate growth in the salinity of 7 ppt. To identi ed the genes and pathways in response to acute high salinity stress, M. nipponense were exposed to the acute high salinity of 25 ppt. Total RNA from hepatopancreas, gills, and muscle tissues was isolated, and then sequenced using high throughput sequencing method.Differentially expressed genes (DGEs) were identi ed, and total of 632, 836, and 1246 DEGs with a cutoff of signi cant two-fold change were differentially expressed in hepatopancreas, gills, and muscle tissues, respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis were conducted. These DEGs were involved in the GO terms of cellular process, metabolic process, membrane, organelle, binding, and catalytic activity. The DEGs of hepatopancreas and gill tissues were mainly enriched in PPAR signaling pathway, longevity regulating pathway, protein digestion and absorption, and the DEGs of muscle tissue in arginine biosynthesis, adrenergic signaling in cardiomyocytes, cardiac muscle contraction, and cGMP-PKG signaling pathway. Real-time PCR conducted with fteen selected DEGs indicated high reliability of digital analysis using RNA-Seq. This work provides a comprehensive insight into the molecular responses to high salinity stress in M. nipponense, which provide a novel contribution to understanding of the molecular mechanisms of adaptation to salinity stress in euryhaline crustaceans.

Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress

“…Through RNA-Seq analysis, several heat-related genes, including heat shock proteins (HSPs) and cytochrome P450 (CYP450), also play a crucial role for heat tolerance in M. nipponense. After the discovery of HSPs, increasing functions were attached to HSPs, such as molecular chaperones in protein folding and unfolding (Fan et al 2022c), and managing the transcription machinery (Cui et al 2014). In this study, both HSP70 and HSP90 were signi cantly induced in the 35 ℃ group which illustrated that when exposed to acute heat stress, the higher expression of HSPs assisted the M. nipponense in relieving the damage caused by acute heat stress (Fig.…”