RNA-sequencing indicates high hemocyanin expression as a key strategy for cold adaptation in the Antarctic amphipod Eusirus cf. giganteus clade g3

Greco, Samuele; D’Agostino, Elisa; Manfrin, Chiara; Gaetano, Anastasia Serena; Furlanis, Gael; Capanni, Francesca; Santovito, Gianfranco; Edomi, Paolo; Giulianini, Piero Giulio; Gerdol, Marco

doi:10.32604/biocell.2021.016121

Cited by 7 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, such adaptations in notothenioids include the expression of antifreeze glycoproteins, encoded by an expanded family of trypsinogen-derived genes, which prevent ice-crystal formation in body fluids [66,67], or the loss of an inducible heat shock response, which facilitates protein folding at sub-zero temperatures [68]. Nonetheless, these scientific developments have not progressed at a similar pace in invertebrates, even though molecular approaches have already shown great potential in uncovering novel cold adaptation strategies, as in the case of Antarctic amphipods [69]. Although A. colbecki is the most abundant bivalve species in Antarctic coastal waters and a key component of the Southern Ocean food web, our current knowledge of the mechanisms that enable this species to survive and thrive at sub-zero temperatures is limited to a handful of studies, none of which has exploited large-scale -omic approaches to date.…”

Section: Multiple a Colbecki Tissues Display A Shared Transcriptomic ...mentioning

confidence: 99%

Comparative Transcriptomic Analysis Reveals Adaptive Traits in Antarctic Scallop Adamussium colbecki

Greco,

Voltarel,

Gaetano

et al. 2023

Fishes

Self Cite

View full text Add to dashboard Cite

Antarctica is the most extreme continent of Earth, with strong winds, freezing temperatures on land, and ocean temperatures constantly below 0 °C. Nonetheless, the Antarctic Ocean is home to an astounding diversity of living organisms that adapted to the multiple challenges posed by this environment via a diverse set of evolutionary traits. Although the recent advancements in sequencing technologies clarified the molecular bases of such adaptations in Antarctic fishes, little information is available for Antarctic invertebrates. In this preliminary study, we address this knowledge gap with a comparative transcriptomic approach to obtain insights into some of the adaptations that allow the Antarctic scallop Adamussium colbecki to survive and thrive in the freezing waters of the Antarctic Ocean. Despite some limitations, our analyses highlighted significant over-expression of genes involved in regulation of mRNA transcription, maturation, and degradation, which might compensate for a reduced efficiency of these processes at low temperatures. Other alterations detected in the Antarctic scallop transcriptome include enhanced expression of genes that regulate degradation of misfolded protein products and allow maintenance of cytoskeletal structure and function at subzero temperatures. Altogether, these observations support the presence of multiple previously unreported molecular adaptive traits in A. colbecki, which have important implications for our understanding of adaptation of this important component of the Antarctic trophic chain to such an extreme, but stable environment.

show abstract

Section: Multiple a Colbecki Tissues Display A Shared Transcriptomic ...mentioning

confidence: 99%

Comparative Transcriptomic Analysis Reveals Adaptive Traits in Antarctic Scallop Adamussium colbecki

Greco,

Voltarel,

Gaetano

et al. 2023

Fishes

Self Cite

View full text Add to dashboard Cite

show abstract

“…To reduce redundancy, only the longest isoform assembled for each gene model was considered for subsequent analyses. Spurious transcripts resulting from exogenous contaminant RNAs (expected to be present due to the filter-feeding habits of C. kusceri) and those derived from poorly expressed and likely fragmented endogenous transcripts unlikely to carry out a biologically relevant role were detected as follows: Salmon v.1.2.1 [23] was used to calculate the coverage of all contigs (based on the combined mapping of all libraries), and all the contigs that did not reach the arbitrary threshold of five transcripts per million (TPM) [24] were removed, following the same protocol used in previous studies carried out by our team on aquatic invertebrates [25,26]. This process allowed discarding contigs that may have derived from exogenous contamination (e.g., due to the presence of food particles associated with gills and digestive glands, microbial cells associated with pathogens and/or symbionts, debris mixed with bivalve tissues as a result of filter-feeding, etc.…”

Section: De Novo Assembly Quality Assessment and Annotation Of The Tr...mentioning

confidence: 99%

Variation of Gene Expression in the Endemic Dinaric Karst Cave-Dwelling Bivalve Mollusk Congeria kusceri during the Summer Season

Scapolatiello,

Manfrin,

Greco

et al. 2023

Diversity

Self Cite

View full text Add to dashboard Cite

The cave systems of the Neretva River basin in the Dinaric Karst are home to Congeria kusceri, one of the very few known examples of stygobiotic bivalve mollusks, which displays several unique life history traits and adaptations that allowed its adaptation to the subterranean environment. This endemic species is undergoing rapid decline, most likely linked with habitat degradation, which might seriously threaten its survival in the next few decades. Unfortunately, the urgent need for effective conservation efforts is hampered by the lack of effective regulations aimed at preserving remnant populations as well as by our limited knowledge of the biology of this species. Although the precise factors underlying the disappearance of C. kusceri from its type locations are not entirely clear, the alteration of seasonal changes in water temperatures and alkalinity is most likely involved, as these are the main drivers of shell growth, spawning, and the onset and progression of the new gametogenic cycle. While these aspects have so far only been studied using morphological and physiological observations, future conservation efforts would certainly benefit from an integrated multidisciplinary approach. Here, using RNA-sequencing, we provide an overview of the modulation of gene expression recorded in five key tissues (the mantle, gonads, gills, adductor muscle, and digestive gland) between early June and late September. We highlight the presence of markedly tissue-specific responses, with the most dramatic changes affecting the gonads, mantle, and gills. We further identified a drastic switch in the use of energy budgets between the two periods, with evidence of ongoing shell growth and high metabolic activity in the mantle and gills at the early time point, followed by a massive redirection of all available energy to the gonads for the generation of new gamete primordia in early autumn.

show abstract

Cold temperatures as a source of stress: seasonality, sand burrowing and hemocyanin levels in Atlantic Canada sandy beach amphipods

2023

View full text Add to dashboard Cite

RNA-sequencing indicates high hemocyanin expression as a key strategy for cold adaptation in the Antarctic amphipod Eusirus cf. giganteus clade g3

Cited by 7 publications

References 47 publications

Comparative Transcriptomic Analysis Reveals Adaptive Traits in Antarctic Scallop Adamussium colbecki

Comparative Transcriptomic Analysis Reveals Adaptive Traits in Antarctic Scallop Adamussium colbecki

Variation of Gene Expression in the Endemic Dinaric Karst Cave-Dwelling Bivalve Mollusk Congeria kusceri during the Summer Season

Cold temperatures as a source of stress: seasonality, sand burrowing and hemocyanin levels in Atlantic Canada sandy beach amphipods

Contact Info

Product

Resources

About