RNA Sequencing of Porine Ganglia and brainstem v1

Abstract

RNA Se que nci ng of Pori ne G a ngl i a a nd bra i nste m RNA Se que nci ng of Pori ne G a ngl i a a nd bra i nste m Ani ma l s. Ani ma l s. A total of 44 piglets (Yorkshire Landrace breed, 2-3 days of age) were fed commercial milk replacer (Soweena Litter Life) and allowed a 36-48-h acclimation period before interventions began. The University of Florida Animal Care and Use Committee approved all procedures. Procedures were completed in accordance with federal policies and guidelines. A total of 12 piglets were reserved for RNA sequencing experiments (6 male and 6 female). Ai rwa y i nsti l l a ti on Ai rwa y i nsti l l a ti on. After acclimation, piglets were anesthetized with 8% SevoThesia (Henry Schein). The piglets' airways were accessed with a laryngoscope; a laryngotracheal atomizer (MADgic) was passed directly beyond the vocal folds, as previously described to aerosolize either a 500 μl 0.9% saline control or 1% acetic acid in 0.9% saline solution to the airway. This procedure results in widespread distribution of aerosolized solutions throughout the piglet airway, including the lung. Consistent with acid as a cough-evoking stimulus acetic acid induced cough in 90% or greater of the piglets.