RNA-sequencing reveals the metabolism regulation mechanism of sheep skeletal muscle under nutrition deprivation stress

Qin, Jie; Guo, Liang; Li, J.L.; Zhang, F.H.; Zhao, Derui; Du, Rong

doi:10.1016/j.animal.2021.100254

Cited by 1 publication

(1 citation statement)

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“…In the present study, the www.nature.com/scientificreports/ enriched KEGG pathways were related to apoptosis, stress response, glucagon signaling pathways, fructose and mannose metabolism, protein metabolism, and calcium signaling pathways. Qin et al 28 also observed that AMPK, PI3-Akt, MAPK, and FOXO pathways were involved in fat, protein, carbohydrate, and glycolipid metabolism in sheep subjected to nutrition deprivation stress. Studies conducted in magpie birds and duroc pigs have further confirmed that AMPK and MAPK pathways play an important role in maintaining energy homeostasis and physiological stress responses 29,30 .…”

Section: Discussionmentioning

confidence: 98%

RNA-Seq exploration of the influence of stress on meat quality in Spanish goats

Naldurtiker

Batchu

Kouakou

et al. 2022

Sci Rep

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Studies exploring the transcriptome of stress and its effects on meat quality are very limited, particularly in goats. Fifty-four male Spanish goats (8-mo old; BW = 29.7 ± 2.03 kg) were randomly subjected to one of three treatments (TRT; n = 18 goats/treatment): (1) transported for 180 min, (2) transported for 30 min, or (3) held in pens (control) to analyze the transcriptome of stress and meat quality in goats using RNA-seq technology. Blood samples were collected before and after treatment, and meat samples were collected after humane slaughter for stress hormone, meat quality (Longissimus dorsi), and transcriptomic analysis. Plasma epinephrine concentrations were higher (P < 0.01) in 180 min and 30 min groups compared to the control group; however, norepinephrine concentrations were not affected by the treatment. Muscle glycogen concentrations (15 min postmortem) were lower (P < 0.01) in both 30 min and 180 min groups compared to the control group. Calpastatin levels were higher (P < 0.01) in 180 min and 30 min groups than the control group. Warner–Bratzler shear force values of loin chops were the highest in the 180 min group (4 ± 0.15, kg), lowest in the control group (3.51 ± 0.10, kg), and intermediate in the 30 min group (3.78 ± 0.09, kg; P < 0.01) both at day 1 and day 6 aging time. Additionally, desmin levels of day 6 samples were lowest in the control group, highest in 180 min group, and intermediate in 30 min group (P < 0.05). RNA-seq results showed that a total of 10,633 genes were differentially expressed (5194 up regulated; 5439 down regulated) among all comparisons (blood and day 1 and day 6 muscle samples). Among these differentially expressed genes (DEGs), KLF9, AMPK, FOXO3, PTX3, GADD45, PTPN1, CASP7, MAPK4, HSPA12A, and JAK-STAT were probably associated with the effects of stress on skeletal muscle proteins and involved in biological process such as cellular response to corticosteroid stimulus, endoplasmic reticulum stress, insulin resistance, DNA repair, apoptosis, MAPK cascade and regulation of proteolysis. The KEGG analysis revealed that AMPK and JAK-SAT signaling pathways and autophagy were among the top 20 enriched pathways in our treatment comparisons. The results provide an understanding of the genes and pathways involved in stress responses and related changes in postmortem muscle metabolism and meat quality characteristics in goats.

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Section: Discussionmentioning

confidence: 98%