RNA-Templated Semiconductor Nanocrystals

Ma, Nan; Dooley, Chad J.; Kelley, Shana O.

doi:10.1021/ja0638962

Cited by 86 publications

(71 citation statements)

References 12 publications

(13 reference statements)

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“…One promising alternative is the use of biological templates for the synthesis of nanocrystals. Currently, some biological templates, including carbohydrate (Dong and Qian, 2008), peptides (Peelle et al, 2005a), nucleotides (Ma et al, 2006b), and fusion proteins (Nam et al, 2006) have been used to make materials. Biological templates not only guide the nucleation of inorganic materials, but also control the crystal structure and size, under aqueous and ambient conditions.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli

Wang

Zhang

et al. 2011

Journal of Biotechnology

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Quantum dots (QDs) were prepared in genetically engineered Escherichia coli (E. coli) through the introduction of foreign genes encoding a CdS binding peptide. The CdS QDs were successfully separated from the bacteria through two methods, lysis and freezing–thawing of cells, and purified with an anion-exchange resin. High-resolution transmission electron microscopy, X-ray diffraction, luminescence spectroscopy, and energy dispersive X-ray spectroscopy were applied to characterize the as-prepared CdS QDs. The effects of reactant concentrations, bacteria incubation times, and reaction times on QD growth were systematically investigated. Our work demonstrates that genetically engineered bacteria can be used to synthesize QDs. The biologically synthesized QDs are expected to be more biocompatible probes in bio-labeling and imaging.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli

Wang

Zhang

et al. 2011

Journal of Biotechnology

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“…Both nucleic acids sequence and structure have been used to control the properties of lead- and cadmium-containing nanocrystals13,14, and the materials made in this way have been shown to have low cellular toxicity and good properties for cellular imaging13. However, little has been done to functionalize these materials to enable versatile molecular recognition.…”

mentioning

confidence: 99%

One-step DNA-programmed growth of luminescent and biofunctionalized nanocrystals

2008

Self Cite

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Colloidal semiconductor nanocrystals are widely used as lumiphores in biological imaging because their luminescence is both strong and stable, and because they can be biofunctionalized. During synthesis, nanocrystals are typically passivated with hydrophobic organic ligands 1 , so it is then necessary either to replace these ligands or encapsulate the nanocrystals with hydrophilic moieties to make the lumiphores soluble in water. Finally, biological labels must be added to allow the detection of nucleic acids, proteins and specific cell types 2-8 . This multistep process is time-and labour-intensive and thus out of reach of many researchers who want to use luminescent nanocrystals as customized lumiphores. Here, we show that a single designer ligand-a chimeric DNA molecule -can controllably program both the growth and the biofunctionalization of the nanocrystals. One part of the DNA sequence controls the nanocrystal passivation and serves as a ligand, while another part controls the biorecognition. The synthetic protocol reported here is straightforward and produces a homogeneous dispersion of nanocrystal lumiphores functionalized with a single biomolecular receptor. The nanocrystals exhibit strong optical emission in the visible region, minimal toxicity and have hydrodynamic diameters of ∼6 nm, which makes them suitable for bioimaging 4 . We show that the nanocrystals can specifically bind DNA, proteins or cells that have unique surface recognition markers.DNA is well suited to the task of producing customized nanocrystal lumiphores because it is known to serve as a receptor for molecular recognition 9 and as an inert nanocrystal passivator 10-15 . Both nucleic acids sequence and structure have been used to control the properties of lead-and cadmium-containing nanocrystals 13,14 , and the materials made in this way have been shown to have low cellular toxicity and good properties for cellular imaging 13 . However, little has been done to functionalize these materials to enable versatile molecular recognition. An aptamer-based strategy using DNA for both nanoparticle liganding during growth and protein detection has recently been reported 15 ; the toxicities and hydrodynamic radii of these materials were not investigated, and although the proposed NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript application of the strategy was restricted to a single protein, adsorption of non-target proteins was noted. In summary, although promising advances have already been made, a general approach to high-fidelity biomolecular functionalization of nanocrystals capable of specifically binding to a diverse range of targets has never previously been explored.We designed a one-pot synthesis that would allow nucleic acids-functionalized nanocrystals to be prepared that would bind a variety of biomolecular targets. The approach relies on the design of chimeric oligonucleotides that contain two different domains-one that will be liganded to the nanocrystal, and one that will be capable of molecular ...

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“…A narrow distribution around 6 nm diameter particles was found for particles grown with wtRNA, while mtRNA generated a bimodal distribution of 7 and 11.5 nm diameter particles. This is a good illustration that a biomolecule can affect the nanocrystal size (Ma, Dooley, and Kelley 2006).…”

Section: Dna and Rna-mediated Bioinspired Synthesismentioning

confidence: 80%

Bioinspired Synthesis of Organic/Inorganic Nanocomposite Materials Mediated by Biomolecules

Liu¹,

Mallapragada²

2011

On Biomimetics

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RNA-Templated Semiconductor Nanocrystals

Cited by 86 publications

References 12 publications

Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli

Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli

One-step DNA-programmed growth of luminescent and biofunctionalized nanocrystals

Bioinspired Synthesis of Organic/Inorganic Nanocomposite Materials Mediated by Biomolecules

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