RNA transcripts for the quantification of differentiation allow marked improvements in the performance of embryonic stem cell test (EST)

Romero, Andrea C.; Río, Eva del; Vilanova, Eugenio; Sogorb, Miguel A.

doi:10.1016/j.toxlet.2015.08.008

Cited by 14 publications

(5 citation statements)

References 25 publications

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“…7 ) for 5 days. At the end of exposure, cells were lysed, RNA was extracted and retrotranscribed to cDNA, and each gene was amplified and quantified by quantitative real time PCR as previously described [1,5,6] and using to 2 −ΔΔCt calculations [7] and β-actin as a house-keeping control gene The expression of each gene was normalized against the expression of this same gene in the control (non-exposed) cells. The mean±s.d.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…1–7 ). The dose–response plots were used to derive ECD50 or ECD200, which were used as end-points for enhancing the performance of embryonic stem cell methods for testing embryotoxicity [1] .…”

Section: Datamentioning

confidence: 99%

“… There is a necessity to develop in vitro methods for testing embryotoxicity (Romero et al, 2015) [1] . We studied the progress of D3 mouse embryonic stem cells differentiation exposed to model embryotoxicants and non-embryotoxicants chemicals through the expression of biomarker genes.…”

mentioning

confidence: 99%

“…We offer dose response in order to derive the concentration causing either 50% or 200% of expression of the biomarker gene. These records revealed to be a valuable end-point to predict in vitro the embryotoxicity of chemicals (Romero et al, 2015) [1] .…”

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confidence: 99%

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Expression of biomarker genes of differentiation in D3 mouse embryonic stem cells after exposure to different embryotoxicant and non-embryotoxicant model chemicals

et al. 2015

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There is a necessity to develop in vitro methods for testing embryotoxicity (Romero et al., 2015) [1]. We studied the progress of D3 mouse embryonic stem cells differentiation exposed to model embryotoxicants and non-embryotoxicants chemicals through the expression of biomarker genes. We studied a set of 16 different genes biomarkers of general cellular processes (Cdk1, Myc, Jun, Mixl, Cer and Wnt3), ectoderm formation (Nrcam, Nes, Shh and Pnpla6), mesoderm formation (Mesp1, Vegfa, Myo1e and Hdac7) and endoderm formation (Flk1 and Afp). We offer dose response in order to derive the concentration causing either 50% or 200% of expression of the biomarker gene. These records revealed to be a valuable end-point to predict in vitro the embryotoxicity of chemicals (Romero et al., 2015) [1].

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Datamentioning

confidence: 99%

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Expression of biomarker genes of differentiation in D3 mouse embryonic stem cells after exposure to different embryotoxicant and non-embryotoxicant model chemicals

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“…Currently, mESC-based toxicity assays represent a powerful tool for the high-throughput toxicity screening of emerging organic chemicals [ 25 ], for chemical use and exposure guideline purposes. For instance, the US Environmental Protection Agency (EPA) and others developed a toxicity assay based on the monolayer differentiation of mESCs into cardiomyocytes, called mESC adherent cell differentiation and cytotoxicity (ACDC) assay [ 34 ], enabling the high-throughput screening of environmental pollutants, which also gives information on the underlying molecular mechanisms [ 35 ].…”

Section: Development Of Stem Cell Toxicologymentioning

confidence: 99%

Prospects and Frontiers of Stem Cell Toxicology

Liu

Yin

Faiola

2017

Stem Cells and Development

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The development of stem cell biology has revolutionized regenerative medicine and its clinical applications. Another aspect through which stem cells would benefit human health is their use in toxicology. In fact, owing to their ability to differentiate into all the lineages of the human body, including germ cells, stem cells, and, in particular, pluripotent stem cells, can be utilized for the assessment, in vitro, of embryonic, developmental, reproductive, organ, and functional toxicities, relevant to human physiology, without employing live animal tests and with the possibility of high throughput applications. Thus, stem cell toxicology would tremendously assist in the toxicological evaluation of the increasing number of synthetic chemicals that we are exposed to, of which toxicity information is limited. In this review, we introduce stem cell toxicology, as an emerging branch of in vitro toxicology, which offers quick and efficient alternatives to traditional toxicology assessments. We first discuss the development of stem cell toxicology, and we then emphasize its advantages and highlight the achievements of human pluripotent stem cell-based toxicity research.

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A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs

Hodjat,

Abdollahi

2024

Methods in Molecular Biology

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RNA transcripts for the quantification of differentiation allow marked improvements in the performance of embryonic stem cell test (EST)

Cited by 14 publications

References 25 publications

Expression of biomarker genes of differentiation in D3 mouse embryonic stem cells after exposure to different embryotoxicant and non-embryotoxicant model chemicals

Expression of biomarker genes of differentiation in D3 mouse embryonic stem cells after exposure to different embryotoxicant and non-embryotoxicant model chemicals

Prospects and Frontiers of Stem Cell Toxicology

A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs

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