RNAi-mediated knockdown of Xist improves development of the female buffalo (Bubalus bubalis) nuclear transfer embryos

Li, Zhengda; Ruan, Ziyun; Zhao, Xin; Qin, Xiaoxia; Zhang, Jun; Feng, Yun; Lu, Jiaka; Shi, Deshun; Lu, Fenghua

doi:10.1016/j.theriogenology.2022.04.020

Cited by 3 publications

(4 citation statements)

References 21 publications

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“…Additionally, both donor nuclei showed a down-regulation of chromosome-scale expression on the X chromosome. Previous studies have shown that abnormal X-chromosome reprogramming occurs in bovine [38], mouse [39], porcine [40], and buffalo [41] embryos. This is mainly caused by a disruption of Xist expression.…”

Section: Discussionmentioning

confidence: 97%

Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome

Li,

Liu,

Zhou

et al. 2024

Animals

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Oocytes are efficient at reprogramming terminally differentiated cells to a totipotent state. Nuclear transfer techniques can exploit this property to produce cloned animals. However, the overall efficiency is low. The use of umbilical cord mesenchymal stem cells (UC-MSCs) as donor nuclei may increase blastocyst rates, but the exact reasons for this remain unexplored. A single-cell transcriptomic approach was used to map the transcriptome profiles of eight-cell embryos that were in vitro-fertilized and handmade-cloned using umbilical cord mesenchymal stem cells and fibroblasts as nuclear donors. Differences were examined at the chromatin level, the level of differentially expressed genes, the level of histone modifications and the level of DNA methylation. This research provides critical information regarding the use of UC-MSCs as a preferred donor nucleus for nuclear transfer techniques. It also offers unique insights into the mechanism of cellular reprogramming.

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Section: Discussionmentioning

confidence: 97%

Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome

Li,

Liu,

Zhou

et al. 2024

Animals

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“…It has previously been shown that the survival of female embryos after SCNT was higher than that of male embryos. Abnormal DNA methylation patterns (5-methylcytosine-5 mC and 5-hydroxymethylcytosine-5 hmC) were found in cloned buffalo embryos, which may be due to dosage compensation of the X chromosome, XCI (X-chromosome inactivation) at an early stage of embryonic development [ 152 , 153 , 154 ]. The Xist gene (XCI specific transcription gene) is imprinted on the X chromosome and regulates XCI [ 155 ].…”

Section: Methods Of Gene Delivery Successfully Applied In Livestock B...mentioning

confidence: 99%

“…This problem can be solved by suppressing the expression of the Xist gene, as was previously done in mice using gene knock-out or siRNA injection; Xist -siRNA also led to an increase in the birth rate of healthy cloned male piglets [ 158 , 159 , 160 ]. It was shown that siRNA-mediated knock-down of the Xist gene effectively inhibits gene expression and promotes the development of SCNT-cloned female buffalo embryos [ 153 ].…”

Section: Methods Of Gene Delivery Successfully Applied In Livestock B...mentioning

confidence: 99%

Perspectives in Genome-Editing Techniques for Livestock

Popova

Bets

Kozhevnikova

2023

Animals

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Genome editing of farm animals has undeniable practical applications. It helps to improve production traits, enhances the economic value of livestock, and increases disease resistance. Gene-modified animals are also used for biomedical research and drug production and demonstrate the potential to be used as xenograft donors for humans. The recent discovery of site-specific nucleases that allow precision genome editing of a single-cell embryo (or embryonic stem cells) and the development of new embryological delivery manipulations have revolutionized the transgenesis field. These relatively new approaches have already proven to be efficient and reliable for genome engineering and have wide potential for use in agriculture. A number of advanced methodologies have been tested in laboratory models and might be considered for application in livestock animals. At the same time, these methods must meet the requirements of safety, efficiency and availability of their application for a wide range of farm animals. This review aims at covering a brief history of livestock animal genome engineering and outlines possible future directions to design optimal and cost-effective tools for transgenesis in farm species.

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“…The efficiency of SCNT-based cloning in mammals remains at relatively or extremely low levels, and it appears to differ in a species-specific manner. To improve the SCNT efficiency, extensive efforts are needed to precisely identify the epigenetic factors and molecular mechanisms involved in the reprogramming of the nuclear donor cell’s (NDC’s) genome in somatic cell cloned embryos ( Cao et al, 2020 ; Liu et al, 2020 ; Feng et al, 2022 ; Li et al, 2022 ). Thus far, the provenance and type of NDCs have been shown to influence the capability of donor genomic DNA to be epigenetically reprogrammed in the oocyte cytoplasm during SCNT ( Samiec and Skrzyszowska, 2010 , 2013 ; Sadeesh et al, 2016 ; Wiater et al, 2021b ; Xu et al, 2021 ; Zhang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Epigenetic manipulation to improve mouse SCNT embryonic development

Sun

2022

Front. Genet.

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Cloned mammals can be achieved through somatic cell nuclear transfer (SCNT), which involves reprogramming of differentiated somatic cells into a totipotent state. However, low cloning efficiency hampers its application severely. Cloned embryos have the same DNA as donor somatic cells. Therefore, incomplete epigenetic reprogramming accounts for low development of cloned embryos. In this review, we describe recent epigenetic barriers in SCNT embryos and strategies to correct these epigenetic defects and avoid the occurrence of abnormalities in cloned animals.

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RNAi-mediated knockdown of Xist improves development of the female buffalo (Bubalus bubalis) nuclear transfer embryos

Cited by 3 publications

References 21 publications

Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome

Comparison of Umbilical Cord Mesenchymal Stem Cells and Fibroblasts as Donor Nuclei for Handmade Cloning in Sheep Using a Single-Cell Transcriptome

Perspectives in Genome-Editing Techniques for Livestock

Epigenetic manipulation to improve mouse SCNT embryonic development

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