RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides

Revuelta, Luis; Piulachs, María‐Dolors; Bellés, Xavier; Castañera, Pedro; Ortego, Félix; Díaz-Ruíz, José Ramón; Hernández‐Crespo, Pedro; Tenllado, Francisco

doi:10.1016/j.ibmb.2009.11.001

Cited by 69 publications

(63 citation statements)

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“…In some cases, mutations in ace1 explain better than mutations in ace2 the altered AChE activity observed in association with insecticide resistance (Kono and Tomita, 2006). In Blattella germanica, specific interference of Bgace1 and Bgace2 genes demonstrates that BgAChE1 is responsible for at least 65e75% of the total AChE activity detected in nerve tissues, and specific interference of Bgace1 produces an increased mortality in insects when treated with insecticides that is not produced after specific interference of Bgace2 (Revuelta et al, 2009). Finally, studies on tissue distribution of Bgace1 and Bgace2 transcripts (Kim et al, 2006b), and the abundance of BgAChE1 in comparison with BgAChE2 using specific antibodies (Kim et al, 2010), suggest the major role of BgAChE1 in postsynaptic transmission.…”

Section: Introductionmentioning

confidence: 99%

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle

Revuelta

Ortego

Díaz-Ruíz

et al. 2011

Insect Biochemistry and Molecular Biology

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The Colorado potato beetle (CPB), Leptinotarsa decemlineata is an important economic pest of potato worldwide. Resistance to organophosphates and carbamates in CPB has been associated in some cases to point mutations in the acetylcholinesterase (AChE) gene Ldace2, an orthologue of Drosophila melanogaster Dmace2. In this paper we report cloning and sequencing of Ldace1, an orthologue of Anopheles gambiae Agace1 that was previously unknown in CPB. The Ldace1 coding enzyme contains all residues conserved in a functionally active AChE. Ldace1 is expressed at higher levels (between 2- and 11-fold) than Ldace2 in embryos, in the four larval instars and in adults. Specific interference of Ldace1 by means of dsRNA injection resulted in a reduction of AChE activity to an approximate 50% compared to control, whilst interference of Ldace2 reduced AChE activity to an approximate 85%. Analysis of zymograms of AChE activity after interference indicates that LdAChE1 is the enzyme predominantly responsible for the activity visualised. Interference of Ldace1 in CPB adults caused a significant increase in mortality (43%) as early as three days post-injection (p.i.), suggesting the essential role of Ldace1. Interference of Ldace2 also caused a significant increase in mortality (29%) compared to control, although at seven days p.i. The effect of the interference of Ldace1 on susceptibility to the organophosphate chlorpyrifos points out that LdAChE1 could be a main target for this insecticide. In the light of our results, studies associating resistance in CPB to mutations in Ldace2 should be reviewed, taking into consideration analysis of the Ldace1 gene.

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Section: Introductionmentioning

confidence: 99%

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle

Revuelta

Ortego

Díaz-Ruíz

et al. 2011

Insect Biochemistry and Molecular Biology

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“…1 To date, two AChE genes (ace), ace1 and ace2, which encode AChE1 and AChE2, have been identified in most insects. 2 A recent study conducted using native gel electrophoresis and western blotting confirmed that AChE1 was the main form within the head extract of 67 out of 100 insect species examined. 3 AChE, which plays a critical role in the nervous system, is the main target of organophosphate (OP) and carbamate (CB) compounds for insect pest control.…”

Section: Introductionmentioning

confidence: 92%

Distinct contributions of A314S and novel R667Q substitutions of acetylcholinesterase 1 to carbofuran resistance of Chilo suppressalis Walker

2015

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With the enhancing activity of the R667Q substitution, A314S is the major CsAChE1 substitution responsible for fitness-cost compensation and increased insensitivity to AChE inhibitors. The lower insensitivity of A314S-substituted CsAChE1 to chlorpyrifos-oxon suggests that chlorpyrifos could be an alternative insecticide for managing carbofuran-resistant field C. suppressalis in Taiwan. © 2015 Society of Chemical Industry.

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“…Diverse works correlate the resistance to pesticides with the increase of enzymatic activity, attributing it to punctual mutations, genic duplications and mutations in the target molecule (Mutero et al, 1994;Villatte et al, 2000;Vontas et al, 2001;Vontas et al, 2002;Ortelli et al, 2003;Menozzi et al, 2004;Revuelta et al, 2009;Ying et al, 2013;Chang et al, 2014;Zhao et al, 2014;Xi et al, 2015).…”

Section: Metabolic Resistancementioning

confidence: 99%

“…Revista Bio Ciencias 4(6), Article ID 04.06.01 December 2017 presentes en A. gambiae (Weill et al, 2002;Fournier, 2005), sin embargo, en D. melanogaster la AChE es codificada úni-camente por ace2 (Fournier et al, 1993;Weill et al, 2002;Huchard et al, 2006); estudios en otros insectos que poseen ambos genes, sugieren que ace1 es el gen responsable de una mayor actividad de la AChE (Revuelta et al, 2009;Revuelta et al, 2011).…”

Section: Acetylcholinesterasesunclassified

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Rojas

Bermudez

Cambero-Campos

et al. 2017

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RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides

Cited by 69 publications

References 36 publications

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle

Distinct contributions of A314S and novel R667Q substitutions of acetylcholinesterase 1 to carbofuran resistance of Chilo suppressalis Walker

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