RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies

Windisch, Jörg M.; Schneider, Rainer; Stark, Robert; Weiland, Emilie; Meyers, Gregor; Thiel, Heinz-Jürgen

doi:10.1128/jvi.70.1.352-358.1996

Cited by 77 publications

(34 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…E rns possesses several remarkable features, of which the RNase activity is of particular interest, considering that it is expressed by an RNA virus. E rns has structural similarities with plant T2 RNases which have their optimal catalytic activity at an acidic pH [32] with a preference for cleaving single-stranded RNA [33,34]. This would point on an activity within the endosomal compartment, which is also supported by our data indicating that genomic RNA does not appear to be degraded.…”

Section: Discussionsupporting

confidence: 75%

Efficient Sensing of Infected Cells in Absence of Virus Particles by Blasmacytoid Dendritic Cells Is Blocked by the Viral Ribonuclease Erns

Python¹,

Gerber²,

Suter³

et al. 2013

PLoS Pathog

View full text Add to dashboard Cite

Plasmacytoid dendritic cells (pDC) have been shown to efficiently sense HCV- or HIV-infected cells, using a virion-free pathway. Here, we demonstrate for classical swine fever virus, a member of the Flaviviridae, that this process is much more efficient in terms of interferon-alpha induction when compared to direct stimulation by virus particles. By employment of virus replicon particles or infectious RNA which can replicate but not form de novo virions, we exclude a transfer of virus from the donor cell to the pDC. pDC activation by infected cells was mediated by a contact-dependent RNA transfer to pDC, which was sensitive to a TLR7 inhibitor. This was inhibited by drugs affecting the cytoskeleton and membrane cholesterol. We further demonstrate that a unique viral protein with ribonuclease activity, the viral Erns protein of pestiviruses, efficiently prevented this process. This required intact ribonuclease function in intracellular compartments. We propose that this pathway of activation could be of particular importance for viruses which tend to be mostly cell-associated, cause persistent infection, and are non-cytopathogenic.

show abstract

Section: Discussionsupporting

confidence: 75%

Efficient Sensing of Infected Cells in Absence of Virus Particles by Blasmacytoid Dendritic Cells Is Blocked by the Viral Ribonuclease Erns

Python¹,

Gerber²,

Suter³

et al. 2013

PLoS Pathog

View full text Add to dashboard Cite

show abstract

“…In addition, antibodies which reduce the enzymatic activity of Erns also inhibit infectivity of the virus. This implies an essential role of Erns in the viral life cycle (Schneider et al, 1993;Windisch et al, 1996). E2 is the main target molecule for neutralizing antibodies (Bolin et al, 1988;Donis et al, 1988) and is probably involved in the initial binding of virus particles to the surface of permissive cells (Donis et al, 1988;Xue and Minocha, 1993).…”

Section: Introductionmentioning

confidence: 99%

Bovine Viral Diarrhoea Virus is Internalized by Clathrin‐dependent Receptor‐mediated Endocytosis

Grummer

Grotha

Greiser‐Wilke

2004

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

Bovine viral diarrhoea virus (BVDV) is a pestivirus within the family Flaviviridae. In contrast to the members of the genus flavivirus, nothing is known about the viral entry route for pestiviruses. In this study, the process of BVDV infection following attachment to the cell surface was examined. BVDV clearly co-localizes with clathrin, with early endosome antigen-1 (EEA-1), an early endosome marker, and also with lysosomal-associated membrane protein-2 (LAMP-2), a lysosomal marker. BVDV internalization is inhibited by compounds that block clathrin- but not caveolae-dependent endocytosis. These findings demonstrate that BVDV enters the cells via the clathrin-coated pit pathway.

show abstract

“…Additionally, the E rns acting as a ribonuclease (Windisch et al 1996) may have been released by infected cells into the medium (Ru¨menapf et al 1993). Changes of the culture system (pH changes ±0.15) by viral origin significantly affect embryonic development (Vaijta et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of In Vivo‐ and In Vitro‐produced Porcine Embryos to Classical Swine Fever Virus

Schüürmann¹,

Flögel-Niesmann

Mönnig

et al. 2005

Reprod Domestic Animals

View full text Add to dashboard Cite

The objective of this study was to investigate the susceptibility of in vivo- and in vitro-produced (IVP) porcine embryos to classical swine fever virus (CSFV). IVP zona pellucida (ZP)-intact porcine embryos (n = 721) were co-cultured with CSFV for 120 h. After washing according to the International Embryo Transfer Society guidelines (without trypsin) and transferring embryos to CSFV-susceptible porcine kidney cells (PK15 cell line), no virus was isolated. However, when 88 IVP ZP-intact porcine embryos were co-cultured with CSFV for only 48 h before being transferred to PK15 cells, virus was isolated in three of six replicates. Similarly, 603 in vivo-produced porcine embryos were co-cultured with CSFV for 120 h. Subsequently, CSFV was isolated in eight of 50 groups (16%) and the ability of these to form a blastocyst was significantly reduced when compared with the control group (68.2 +/- 19.9% vs 81.9 +/- 9.7%; p < or = 0.001). In contrast, the development of CSFV-exposed IVP porcine embryos was not affected when compared with control embryos (19.1 +/- 10.8% vs 18.9 +/- 10.6%; p > or = 0.05). After removal of the ZP of IVP embryos and subsequent co-culture with CSFV, the virus was isolated from all groups of embryos. These data suggest that virus replication had occurred in the embryonic cells. In conclusion, data indicate that in vivo- and in vitro-produced ZP-intact porcine embryos differ in their susceptibility to CSFV. Hatched or micro-manipulated embryos may increase the risk of transmission of CSFV by embryo transfer, which has to be confirmed by in vivo tests under isolation conditions.

show abstract

RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies

Cited by 77 publications

References 35 publications

Efficient Sensing of Infected Cells in Absence of Virus Particles by Blasmacytoid Dendritic Cells Is Blocked by the Viral Ribonuclease Erns

Efficient Sensing of Infected Cells in Absence of Virus Particles by Blasmacytoid Dendritic Cells Is Blocked by the Viral Ribonuclease Erns

Bovine Viral Diarrhoea Virus is Internalized by Clathrin‐dependent Receptor‐mediated Endocytosis

Susceptibility of In Vivo‐ and In Vitro‐produced Porcine Embryos to Classical Swine Fever Virus

Contact Info

Product

Resources

About