1998
DOI: 10.1016/s0014-5793(98)00729-7
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RNase P RNA from Prochlorococcus marinus: contribution of substrate domains to recognition by a cyanobacterial ribozyme

Abstract: The molecular organisation of the Prochlorococcus marinus rnpB gene and the catalytic activity of the encoded RNA were characterised. Kinetic parameters for several pre-tRNA substrates were comparable to those from other eubacterial RNase P RNAs, although unusually high cation concentrations were required. The CCA-end of pre-tRNAs is essential for efficient turnover despite the lack of the canonical binding motif in P. marinus RNase P RNA. A trnR gene is located only 38 nt upstream the rnpB 5P end on the compl… Show more

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Cited by 19 publications
(19 citation statements)
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“…In addition, the 5h end of PAC1B rnpB was amplified using primers Pm-F5h and cprp3h-OL. This experiment confirmed a genome arrangement similar to that in SS120 T (Hess et al, 1998), with a gene for tRNA Arg located immediately upstream on the complementary strand (Hess & Scho$ n, 1999). Interestingly, the PAC1A sequence is more similar to NATL2A than NATL2A is to NATL1A and PAC1A is to PAC1B, obtained from the same culture.…”
Section: Bacterial Strains Cultures Of Various Prochlorococcus Strainssupporting
confidence: 73%
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“…In addition, the 5h end of PAC1B rnpB was amplified using primers Pm-F5h and cprp3h-OL. This experiment confirmed a genome arrangement similar to that in SS120 T (Hess et al, 1998), with a gene for tRNA Arg located immediately upstream on the complementary strand (Hess & Scho$ n, 1999). Interestingly, the PAC1A sequence is more similar to NATL2A than NATL2A is to NATL1A and PAC1A is to PAC1B, obtained from the same culture.…”
Section: Bacterial Strains Cultures Of Various Prochlorococcus Strainssupporting
confidence: 73%
“…The corresponding domain in E. coli contains the canonical 5h-GGU-3h motif, binding the substrate via its 3h CCA end. However, despite the obvious replacement of this binding domain by the P15\P16 loop, the pre-tRNA CCA end remains crucial for efficient turnover in Prochlorococcus, as shown previously (Hess et al, 1998). The presence of three consecutive U-U ' mismatches ' in helix P16 of P. marinus subsp.…”
Section: Structural Comparisonsupporting
confidence: 52%
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“…Transcription pattern for the rbcL gene (coding the large subunit of Rubisco protein) was obtained by quantitative real-time polymerase chain reaction (RT-PCR) using the rnpB gene (encoding RNase P RNA) (Hess et al 1998) as an internal control to normalize for total RNA. Gene-specific RT-PCR primers for rbcL, rbcLMED 4-55F (CCTGAATA-TGTCCCCCTCGA) and rbcLMED 4-145R (CCGCTGCT-GCAACTTCTTCT) and for rnpB, rnpBPCC 9511-1F (TTGAGGAAAGTCCGGGCTC) and rnpBPCC 9511-91R (GCGGTATGTTTCTGTGGCACT) were designed from the total genome sequence of Prochlorococcus MED 4 (http:// genome.jgi-psf.org/microbial/) using PrimerExpress software from Applied Biosystems.…”
Section: Parameters Of the Carbon Fixation Versus Light Relationship-mentioning
confidence: 99%