RNF185-AS1 promotes hepatocellular carcinoma progression through targeting miR-221-5p/integrin β5 axis

Abstract

Background: Recently, long noncoding RNAs (lncRNAs) have been reported to play important role in pathogenesis of various cancers. However, the function of RNF185-AS1 in hepatocellular carcinoma (HCC) metastasis has not been well investigated. The present study aims to explore the role and mechanism of RNF185-AS1 in hepatocellular carcinoma metastasis.Methods: The RNF185-AS1 expression in HCC cells and tissues was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The functional effects of RNF185-AS1 on tumor cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) were assessed by Cell Counting Kit-8 (CCK8) assay, colony formation assay, transwell assay and Western blot. The luciferase reporters assay, RNA-binding protein immunoprecipitation assay, qRT-PCR and Western blot were performed to explore and con rm the interaction between RNF185-AS1 and miR-221-5p and integrin β5. The role of RNF185-AS1 in tumor progression was explored through in vivo experiments.Results: RNF185-AS1 was highly expressed in HCC tissues and cell lines. High levels of RNF185-AS1 was correlated with advanced TNM stage, distant metastasis and a poorer overall survival rate. RNF185-AS1 knockdown inhibited cell proliferation, migration, invasion and EMT. Additionally, RNF185-AS1 acted as a sponge for miR-221-5p and integrin β5 was identi ed as a target gene of miR-221-5p. Rescue assays showed that miR-221-5p inhibitor or integrin β5 overexpression rescued the function of RNF185-AS1 knockdown on cell proliferation, migration, invasion and EMT. Moreover, we found that RNF185-AS1 knockdown inhibited tumor metastases in xenograft tumor mouse model. Conclusion:Our ndings demonstrated that RNF185-AS1 promoted cell EMT and migration by regulating miR-221-5p/integrin β5 axis in HCC.

Predictors based on cuproptosis closely related to angiogenesis predict colorectal cancer recurrence

“…Relevant research indicates that DLEU1 , as a protective predictor, is a candidate gene of tumor suppressor involved in B-cell chronic lymphocytic leukemia ( 38 ). RNF185-AS1 , in contrast, has the effect of promoting proliferation and migration in thyroid carcinoma and liver cancer ( 39 , 40 ). The effect of the two predictors in tumor progression confirmed their influence on the prediction of survival probability.…”

Inhibition of hepatocellular carcinoma growth via modulation of the miR-221/SOX11 axis by curcumin and berberine

“…Recently, miR-221 has been extensively studied as a tumor-promoting factor ( Corra et al, 2021 ; Di Paolo et al, 2021 ). It also acts as a biomarker of HCC and mediates its progression ( Huang et al, 2021a ). The significant downregulation of miR-221 following CUR and BBR treatment, as well as the enhanced effect of CUR-BBR combination therapy, suggests a pivotal role of miR-221 in the inhibitory effects of CUR-BBR on HCC progression, which is consistent with a previous study ( Liu et al, 2022a ).…”

Hepatitis B Virus Surface Antigen Promotes Stemness of Hepatocellular Carcinoma through Regulating MicroRNA-203a

“…Most candidate microRNAs selected from the microarray were down-regulated in HBVpositive HCC tissues, including miR-30b-5p, miR-98-5p, miR-148a-3p, and miR-221-5p, which have been shown to regulate the development of HCC. [33][34][35][36][37] miR-203a, with the most significant difference, has been reported to in- hibit the growth and metastasis of HCC by regulating molecules such as interleukin-24 and matrix metalloprotein-2, and also negatively regulates the epithelial-mesenchymal transition process caused by HCV core protein. [38][39][40][41] However, the relationship between HBV and miR-203a has not yet been described, and most studies have focused on cell proliferation and cell cycle changes rather than on tumor stemness.…”