The purpose of this study is to demonstrate methodology for characterization of a plug-in hybrid electric vehicle (PHEV), taking into account gasoline and electricity consumption and emissions associated with each. Field measurements were made of a Toyota Prius with 1.5 liter gasoline engine, Hybrid Synergy Drive (HSD) system with an original battery, and retrofitted Hymotion plug-in system with a second battery. The PHEV initially operates in charge-depleting mode (CD) until the Hymotion battery charge reaches a set point, after which it operates in chargesustaining mode (CS) using only the original battery. Three systems were used for in-use monitoring of the PHEV: (a) electronic download from the hybrid control system interface for factors such as battery charge, voltage, and current, and on-board diagnostic (OBD) data such as engine RPM, manifold absolute pressure, intake air temperature, road speed, and others; (b) portable emission monitoring system (PEMS) measurement of exhaust gas concentrations; and (c) GPS monitoring of coordinates and of altitude using a barometric altimeter. These data were used to characterize the activity of the PHEV, the energy flow associated with the batteries and diesel engine, and the tailpipe emissions. Results are presented based on in-use data collection for real-world driving cycles, in order to demonstrate methodology for integrated analysis of a plug-in hybrid system. Fuel economies for CD and CS modes were approximately 60 and 40 mpg. The indirect electricity emission factors were estimated based on EPA eGRID and National Emission Inventory data. An engine load-based model based on vehicle-specific power (VSP) was developed to explain variation in battery current, fuel use and emission rates based on the real-world data.