Robiginitalea aestuariiviva sp. nov. isolated from sediment of tidal flat located in Zhejiang, PR China

Abstract

A Gram-stain-negative, strictly aerobic and rod- to coccoid-shaped bacterium, designated as strain M366T, was isolated from coastal sediment of Jiaoshanjiao, Zhejiang Province, PR China (121°54′ E 29 °38′ N). The draft genome of strain M366T was 3 225 479 bp long (with 55.6 mol% G+C content) and assembled into four contigs. The N50 value was 563 270 bp and the genomic completeness and contamination were estimated to be 99.34 and 0.05 %, respectively. Colonies of strain M366T were yellow-orange, 1 mm in diameter, round, opaque, smooth and convex after incubation on marine agar at 30 °C for 3 days. Cells were catalase-positive but oxidase-negative. Strain M366T was observed to grow at 20–40 °C (optimum, 30 °C), pH 5.5–9.0 (optimum, pH 6.5–7.0) and with 0.5–8.0 % (w/v) NaCl (optimum, 2.5 %). Strain M366T shown highest 16S rRNA gene sequence similarity of 98.1 % to Robiginitalea sediminis O458T, 95.6–95.9 % to other type strains of the genus Robiginitalea and below 93 % to other genera. The average nucleotide identity and digital DNA–DNA hybridization values between strain M366T and its closely related Robiginitalea species were 71.1–75.9 % and 17.5–19.0 %. Menaquinone-6 was the only respiratory quinone. The major fatty acids (>10 %) were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 1 (iso-C15 : 1 h and/or C13 : 0 3-OH). The main polar lipids included phosphatidylethanolamine, two unidentified phospholipid, two unidentified aminophospholipid, one unidentified glycolipid and five unidentified lipids. According to the above results, Robiginitalea aestuariiviva sp. nov. is proposed and the type strain is M366T (=KCTC 92866T=MCCC 1K04524T=CGMCC 1.61708T).

Microbulbifer magnicolonia sp. nov., isolated from sediment of tidal flat located in Zhoushan, China

“…Growth at different temperatures (4, 10, 15, 20, 25, 27, 30, 37, 40, 45 °C) was determined using MB. To determine the optimal pH range for growth, MB was used and adjusted to pH 5.0–10.0 (at 0.5 pH unit intervals) by adding the following buffers: 2-( N -morpholino) ethane sulphonic acid (pH 5.0–5.5); 3-morpholinopropanesulfonic acid (pH 6.0–7.5); Tricine buffer (pH 8.0–8.5); and N- cyclohexyl-3-aminopropanesulphonic acid (pH 9.0–10.0) [10]. The tolerance to different NaCl concentrations (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 6.0, 8.0, 10.0, and 12.0 %, w/v) was tested in NaCl-free MB, which was prepared according to the MB formula but without NaCl.…”