Objective: The purpose of this study was to compare clinical and surgical outcomes of robotic single-site hysterectomy (RSSH) and robot-assisted multiport hysterectomy (RH) in benign disease. Methods: We retrospectively reviewed the medical records of 38 women who underwent RSSH (N= 12) or RH (N= 26) for the treatment of benign uterine disease between June 2015 and November 2017. Results: There were no intergroup differences in parity, comorbidities, and number of previous abdominal surgery. Mean age was older (49.5± 5.05 years vs. 44.4± 3.54 years, P= 0.001) and mean body mass index was higher (27.4± 2.47 kg/m 2 vs. 25.3± 3.12 kg/ m 2 , P = 0.045) in RSSH group than RH group. Surgical outcomes, including operative time (165.0 minutes vs. 159.2 minutes, P= 0.727), estimated blood loss (115.8± 33.15 mL vs. 108.1± 56.42 mL, P= 0.662), uterus weight (445.9± 157.21 g vs. 374.5± 197.91 g, P= 0.291), postoperative hospital stay (5.4± 0.51 days vs. 5.8± 1.20 days, P= 0.289), postoperative hemoglobin change in day 1 (1.8 ± 0.89 g/dL vs. 1.4 ± 1.53 g/dL, P = 0.431) and day 3 (2.1 ± 1.32 g/dL vs. 1.7 ± 1.83 g/dL, P = 0.601), and perioperative complications did not significantly differ between two groups. The use of additional analgesics after 6 hours, 24 hours, and 48 hours, and mean NRS score after 6 hours, 24 hours, and 48 hours were not significantly different between two groups. Conclusion: RSSH might be an effective and safe alternative to RH, even if in older and/or obese women with large uteri.