Robotic digital microfluidics: a droplet-based total analysis system

Kiani, Mohammad Javad; Dehghan, Amin; Saadatbakhsh, Mohammad; Asl, Shahin Jamali; Nouri, Mahtab; Pishbin, Esmail

doi:10.1039/d2lc00849a

Cited by 11 publications

(6 citation statements)

References 39 publications

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“…Subsequently, migrating droplets merge at the actuation range and progress further for fluorometric detection. In other work, Kiani et al developed an automated and cost-effective digital microfluidic platform for precise mechanical manipulation of the micro/nanoliter droplets . This experimental platform incorporates a robotic arm that is equipped with multiple actuators for dispensing and manipulating droplets on a superhydrophobic cartridge.…”

Section: Different Types Of Sensing Systems With Elements Of Automationmentioning

confidence: 99%

“…In other work, Kiani et al developed an automated and cost-effective digital microfluidic platform for precise mechanical manipulation of the micro/nanoliter droplets. 94 This experimental platform incorporates a robotic arm that is equipped with multiple actuators for dispensing and manipulating droplets on a superhydrophobic cartridge. Their system is also integrated with magnetic and heating modules that facilitate particle manipulation and droplet heating.…”

Section: Different Types Of Sensing Systems With Elements Of Automationmentioning

confidence: 99%

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Automation and Computerization of (Bio)sensing Systems

Raju,

Elpa,

Urban

2024

ACS Sens.

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Sensing systems necessitate automation to reduce human effort, increase reproducibility, and enable remote sensing. In this perspective, we highlight different types of sensing systems with elements of automation, which are based on flow injection and sequential injection analysis, microfluidics, robotics, and other prototypes addressing specific real-world problems. Finally, we discuss the role of computer technology in sensing systems. Automated flow injection and sequential injection techniques offer precise and efficient sample handling and dependable outcomes. They enable continuous analysis of numerous samples, boosting throughput, and saving time and resources. They enhance safety by minimizing contact with hazardous chemicals. Microfluidic systems are enhanced by automation to enable precise control of parameters and increase of analysis speed. Robotic sampling and sample preparation platforms excel in precise execution of intricate, repetitive tasks such as sample handling, dilution, and transfer. These platforms enhance efficiency by multitasking, use minimal sample volumes, and they seamlessly integrate with analytical instruments. Other sensor prototypes utilize mechanical devices and computer technology to address real-world issues, offering efficient, accurate, and economical real-time solutions for analyte identification and quantification in remote areas. Computer technology is crucial in modern sensing systems, enabling data acquisition, signal processing, real-time analysis, and data storage. Machine learning and artificial intelligence enhance predictions from the sensor data, supporting the Internet of Things with efficient data management.

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Section: Different Types Of Sensing Systems With Elements Of Automationmentioning

confidence: 99%

Section: Different Types Of Sensing Systems With Elements Of Automationmentioning

confidence: 99%

Automation and Computerization of (Bio)sensing Systems

Raju,

Elpa,

Urban

2024

ACS Sens.

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“…LHRs, exemplified by Flow Robotics robots, streamline the analysis of tests by executing laboratory protocols without supervision [ 12 ]. Automated laboratory workflows, such as ELISA assays using cartridges [ 13 ], and robotic digital microfluidics for urinalysis tests on disposable superhydrophobic cartridges [ 14 ], often lack open-source software interfaces, hindering protocol customization, interoperability and rapid adaptability, which are crucial in cases of epidemics [ 15 ]. High procurement and maintenance costs and specific labware requirements also hinder accessibility [ 4 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

An Automated Versatile Diagnostic Workflow for Infectious Disease Detection in Low-Resource Settings

Urrutia Iturritza,

Mlotshwa,

Gantelius

et al. 2024

Micromachines

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Laboratory automation effectively increases the throughput in sample analysis, reduces human errors in sample processing, as well as simplifies and accelerates the overall logistics. Automating diagnostic testing workflows in peripheral laboratories and also in near-patient settings -like hospitals, clinics and epidemic control checkpoints- is advantageous for the simultaneous processing of multiple samples to provide rapid results to patients, minimize the possibility of contamination or error during sample handling or transport, and increase efficiency. However, most automation platforms are expensive and are not easily adaptable to new protocols. Here, we address the need for a versatile, easy-to-use, rapid and reliable diagnostic testing workflow by combining open-source modular automation (Opentrons) and automation-compatible molecular biology protocols, easily adaptable to a workflow for infectious diseases diagnosis by detection on paper-based diagnostics. We demonstrated the feasibility of automation of the method with a low-cost Neisseria meningitidis diagnostic test that utilizes magnetic beads for pathogen DNA isolation, isothermal amplification, and detection on a paper-based microarray. In summary, we integrated open-source modular automation with adaptable molecular biology protocols, which was also faster and cheaper to perform in an automated than in a manual way. This enables a versatile diagnostic workflow for infectious diseases and we demonstrated this through a low-cost N. meningitidis test on paper-based microarrays.

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“…11,12 Several types of forces can be used for manipulating the fluids and particles in micro scales. [13][14][15][16] The motion of fluids in centrifugal microfluidics is controlled by centrifugal, corio-lis and Euler forces generated by the rotation of the disc. The angular velocity, acceleration rate, rotating direction of the disc, and the geometry of the channel are critical parameters for controlling fluid flow from the center toward the outer side of the CD.…”

Section: Introductionmentioning

confidence: 99%