Robotized incubation system for monitoring gases (O2, NO, N2O N2) in denitrifying cultures

Molstad, Lars; Dörsch, Peter; Bakken, Lars R.

doi:10.1016/j.mimet.2007.08.011

Cited by 241 publications

(262 citation statements)

References 20 publications

Supporting

Mentioning

260

Contrasting

Unclassified

Order By: Relevance

“…Subsamples (1 mL) for analysis of CO2, O2 and CH4 in headspace were taken at hours 40, 140, 300 and 550. Gas concentrations were analyzed, as described above, directly in the headspace by using an automatic incubator set up similar to that described in an earlier study [20]. After incubation, the samples were freeze-dried to determine the dry weight.…”

Section: Ch4 and Co2 Production Potentialsmentioning

confidence: 99%

Carbon Dioxide and Methane Formation in Norway Spruce Stems Infected by White-Rot Fungi

Hietala

Dörsch

Kvaalen

et al. 2015

Forests

Self Cite

View full text Add to dashboard Cite

Globally, billions of tons of carbon sequestered in trees are annually recycled back to the atmosphere through wood decomposition by microbes. In Norway, every fifth Norway spruce shows at final harvest infection by pathogenic white-rot fungi in the genera Heterobasidion and Armillaria. As these fungi can mineralize all components of wood, we predicted that they have a significant carbon footprint. Gas samples taken from infected stems were analyzed for CO2 and CH4 concentrations, and wood samples from different parts of the decay columns were incubated under hypoxic (4% O2) and anoxic laboratory conditions. In spring and summer the stem concentrations of CO2 were generally two times higher in trees with heartwood decay than in healthy trees. For most of the healthy trees and trees with heartwood decay, mean stem concentrations of CH4 were comparable to ambient air, and only some Armillaria infected trees showed moderately elevated CH4. Consistently, low CH4 production potentials were recorded in the laboratory experiment. Up-scaling of CO2 efflux due to wood decay in living trees suggests that the balance between carbon sequestration and emission may be substantially influenced in stands with high frequency of advanced root and stem heartwood decay.

show abstract

Section: Ch4 and Co2 Production Potentialsmentioning

confidence: 99%

Carbon Dioxide and Methane Formation in Norway Spruce Stems Infected by White-Rot Fungi

Hietala

Dörsch

Kvaalen

et al. 2015

Forests

Self Cite

View full text Add to dashboard Cite

show abstract

“…Soils were incubated at 25°C in an incubator under dark conditions for 3 days. Four specific flasks were used to determine N 2 O and CO 2 concentrations in the headspace at time zero and subsequently every 12 h. At 12, 36, and 72 h, three randomized replicates of the reaming flasks were selected, and soils in these bottles were sampled to assess the soil DEA by using a robotize incubation system (Molstad et al 2007). DEA was determined in soil slurries amended with unlimited NO 3 − by anaerobic incubation (Myrold and Tiedje 1985;Well et al 2005).…”

Section: Soil Samplingmentioning

confidence: 99%

“…The N 2 O, CO 2 , and N 2 concentrations were analyzed with an Agilent 7890 gas chromatography (Santa Clara, CA, USA) equipped with a PLOT column and a Molsieve column, as well as three detectors: a flame ionization detector, a thermal conductivity detector, and an electron capture detector. The incubation system (Molstad et al 2007) was coupled to this gas chromatography through an autosampler, therefore, allowing direct and sequential measurement of headspace gasses.…”

Section: Soil and Gas Measurementsmentioning

confidence: 99%

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

Deng

Rensing

et al. 2013

Environ Sci Pollut Res

View full text Add to dashboard Cite

Soils in urban areas are subjected to compaction with accelerating urbanization. The effects of anthropogenic compaction on urban soil denitrification are largely unknown. We conducted a study on an urban park soil to investigate how compaction impacts denitrification. By using 15 N labeling method and acetylene inhibition technique, we performed three coherent incubation experiments to quantify denitrification in compacted soil under both aerobic and anaerobic conditions. Uncompacted soil was set as the control treatment. When monitoring soil incubation without extra substrate, higher nitrous oxide (N 2 O) flux and denitrification enzyme activity were observed in the compacted soil than in the uncompacted soil. In aerobic incubation with the addition of K 15 NO 3 , N 2 O production in the compacted soil reached 10.11 ng N h −1 g −1 as compared to 0.02 ng N h −1 g −1 in the uncompacted soil. Denitrification contributed 96 % of the emitted N 2 O in the compacted soil and 36 % of the emitted N 2 O in the uncompacted soil; total denitrification rate was higher in the compacted soil (up to 79.35 ng N h −1 g −1 ) than in the uncompacted soil (0.11 ng N h −1 g −1 ). Under anaerobic incubation with the addition of K 15 NO 3 , no statistical difference in total N losses and 15 N-(N 2 O+N 2 ) flux between the uncompacted soil and the compacted soil was detected. Compaction promoted soil denitrification and may impact urban N biogeochemical cycling.

show abstract

“…The injection system was back-flushed by He 6.0 before each sampling to minimise memory effects. Details of the autosampler and injection system are given in Molstad et al (2007). N 2 O emission rates were calculated from the linear or quadratic change in chamber gas concentration using the equation…”

Section: N 2 O Flux Sampling and Calculationmentioning

confidence: 99%

N<sub>2</sub>O emission from organic barley cultivation as affected by green manure management

et al. 2012

View full text Add to dashboard Cite

Abstract. Legumes are an important source of nitrogen in stockless organic cereal production. However, substantial amounts of N can be lost from legume-grass leys prior to or after incorporation as green manure (GM). Here we report N 2 O emissions from a field experiment in SE Norway exploring different green manure management strategies: mulching versus removal of grass-clover herbage during a whole growing season and return as biogas residue to a subsequent barley crop. Grass-clover ley had small but significantly higher N 2 O emissions as compared with a nonfertilised cereal reference during the year of green manure (GM) production in 2009. Mulching of herbage induced significantly more N 2 O emission (+0.37 kg N 2 O-N ha −1 ) throughout the growing season than removing herbage. In spring 2010, all plots were ploughed (with and without GM) and sown with barley, resulting in generally higher N 2 O emissions than during the previous year. Application of biogas residue (60 kg NH + 4 -N + 50 kg organic N ha −1 ) before sowing did not increase emissions neither when applied to previous ley plots nor when applied to previously unfertilised cereal plots. Ley management (mulching vs. removing biomass in 2009) had no effect on N 2 O emissions during barley production in 2010. In general, GM ley (mulched or harvested) increased N 2 O emissions relative to a cereal reference with low mineral N fertilisation (80 kg N ha −1 ). Based on measurements covering the growing season 2010, organic cereal production emitted 95 g N 2 O-N kg −1 N yield in barley grain, which was substantially higher than in the cereal reference treatment with 80 kg mineral N fertilisation (47 g N 2 O-N kg −1 N yield in barley grain).

show abstract

Robotized incubation system for monitoring gases (O2, NO, N2O N2) in denitrifying cultures

Cited by 241 publications

References 20 publications

Carbon Dioxide and Methane Formation in Norway Spruce Stems Infected by White-Rot Fungi

Carbon Dioxide and Methane Formation in Norway Spruce Stems Infected by White-Rot Fungi

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

N<sub>2</sub>O emission from organic barley cultivation as affected by green manure management

Contact Info

Product

Resources

About

Robotized incubation system for monitoring gases (O2, NO, N2O N2) in denitrifying cultures

Cited by 241 publications

References 20 publications

Carbon Dioxide and Methane Formation in Norway Spruce Stems Infected by White-Rot Fungi

Carbon Dioxide and Methane Formation in Norway Spruce Stems Infected by White-Rot Fungi

Compaction stimulates denitrification in an urban park soil using 15N tracing technique

N&lt;sub&gt;2&lt;/sub&gt;O emission from organic barley cultivation as affected by green manure management

Contact Info

Product

Resources

About

N<sub>2</sub>O emission from organic barley cultivation as affected by green manure management