This paper presents a problem of observer-based adaptive fuzzy predefined performance control of a class of nonlinear pure-feedback systems with input delay and unknown control direction. Compared with the existing research, a novel predefined performance controller is proposed, which relaxes the assumption that the initial error is known. In addition, it is difficult to design the controllers due to input delay and nonaffine properties of the pure-feedback systems, which can be simplified by Pade approximation. Moreover, dynamic surface control and Nussbaum functions are applied to overcome the calculation explosion caused by repeated differentiations and unknown control direction, respectively. Based on the above methods, an adaptive fuzzy predefined performance controller is proposed, and it is proved that all the signals of a closed-loop system are semiglobally uniformly ultimately bounded (SGUUB). The tracking errors converge within a predefined range, while the observer estimation errors converge within a small zero region. Finally, the simulation results demonstrate the effectiveness of the proposed control system.