Robust estimation of 5-ALA-induced PpIX multiple-wavelength excitation fluorescence spectroscopy for improving glioma classification

Robust estimation of 5-ALA-induced PpIX contributions in multiple-wavelength excitation fluorescence spectroscopy to improve intraoperative glioma detection: application on clinical data

“…In the present work, we perform the comparison of the separability between healthy and tumoral categories on real clinical data between a state-of-the-art model described in 3 and our model. 1,2 We demonstrated a reduction of the fit residual by 95% in comparison with the reference model. 3…”

5-ALA induced PpIX fluorescence spectroscopy in neurosurgery: a review

“…Indeed, choice of the number and type of excitation sources [light emitting diodes (LED), laser diode, spectrally filtered broadband source] is crucial to the spectral selectivity of fluorescence excitation. Indeed, LEDs have a higher spectral full width half maximum (FWMH) than laser diodes, which in the case of several spectrally closed excitation sources can lead to a loss of selectivity in fluorophore excitation ( Gautheron et al, 2023a ).…”

Experimental decorrelation requirement for robust estimation of fluorophores using multiple-wavelength excitation fluorescence spectroscopy

“…where m, m ′ , S 1 , S 2 , η 1 , η 2 , η ′ 1 and η ′ 2 are given. In order for the two equations of 2 to be jointly exploitable, as in the method described in the article, 16 the measurements m and m ′ must contain complementary information, i.e. they must be as orthogonal as possible.…”

“…We therefore consider that the criterion of decorrelation of m and m ′ is a prerequisite for the use of the multiple wavelength excitation fluorescence spectral unmixing method. 16 This quantitative criterion will be used as a metric to compare the quality of the measurements generated by SystemA and SystemB and then used by our multiple wavelength excitation fluorescence spectral unmixing method. 1 are represented by the light blue, resp.…”

“…These important crosstalks largely impact specificity. In a previous work, 16 we introduce a novel method to estimate the PpIX fluorescence-related biomarkers contribution. As opposed to state of the art, several excitation wavelengths are used.…”