Robust fluorescence sensing platform for detection of CD44 cells based on graphene oxide/gold nanoparticles

Jeong, Ha Young; Baek, Seung Hun; Chang, Sung‐Jin; Cheon, Seon Ah; Park, Tae Jung

doi:10.1016/j.colsurfb.2015.07.083

Cited by 22 publications

(9 citation statements)

References 46 publications

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“…The regression equation is | I | (μA) = −0.018 + 0.216 lg C (cells/mL), R 2 = 0.994. The limit of detection (LOD) is calculated to be 6 cells/mL (defined as a signal-to-noise ratio of three), presenting an improved sensitivity compared to the existing methods. , Moreover, relative standard deviations (RSDs) for repeated measurements were all within 10%, and the average of the RSDs was 4.19%, implying quite good reproduction of our methods.…”

Section: Results and Discussionmentioning

confidence: 82%

Self-Assembling Peptide-Based Multifunctional Nanofibers for Electrochemical Identification of Breast Cancer Stem-like Cells

et al. 2019

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Cancer stem-like cells are closely related with the development and metastasis of tumors. Herein, an electrochemical method is proposed to identify stem-like cells in breast tumor. The core concept of the method is the use of multifunctional nanofibers (MNFs), which are synthesized through facile self-assembly of peptide probes. MNFs can perform three functions, specifically targeting surface biomarker to identify stem-like cells, recruiting silver nanoparticles (AgNPs) to generate electrochemical signals, and providing large amounts of reaction sites to amplify signals. Specially, breast cancer stem cells (BCSCs) are first captured by nucleolin aptamer immobilized on the electrode surface and then selectively recognized by MNFs through the binding with CD44, thereby offering a large number of azide groups for signal labeling. By tracing electrochemical signals from MNF-recruited AgNPs, the method demonstrates to detect target cells as low as 6 cells/mL within a wide linear range from 10 to 5 × 105 cells/mL. Moreover, the method can not only recognize BCSCs with high selectivity in complex environment but also monitor drug-induced stemness changes with high sensitivity, providing promising prospective clinic applications in the future.

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Section: Results and Discussionmentioning

confidence: 82%

Self-Assembling Peptide-Based Multifunctional Nanofibers for Electrochemical Identification of Breast Cancer Stem-like Cells

et al. 2019

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“…[127k] The quenched fluorescence of the target complementary sequence conjugated up conversion NPs (UCNPs) was regained in the presence of target mRNA (quenching was due to adsorption of NPs on GO). The fluorescence quenching efficiency of GO can be enhanced by the formation of hybrid materials, such GO‐AuNPs . The AuNP modified GO exhibited higher fluorescence quenching of tetramethyl‐6‐carboxy‐rhodamine (TAMRA) dye.…”

Section: Nanomaterial‐based Cancer Sensing Systemsmentioning

confidence: 99%

Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Kumar

Kukkar

Hashemi

et al. 2019

Adv Funct Materials

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Cancer is the second most common cause of death in the world. The principal limitations thus far encountered in the clinical practice of probing cancer are diverse and include low sensitivity, time consumption, bulkiness, and cost. In this respect, nanomaterial (NM)-based sensing techniques are recognized as a superior alternative to efficiently resolve such limitations. A better understanding of NM-based sensing platforms is thus important so that these novel avenues can easily be explored for clinical applications. These platforms have the merits of high sensitivity, high specificity, rapid response, and easy-to-read signals. This review offers a comprehensive survey of NM-based advanced cancer-sensing techniques and will help the scientific community establish optimum sensing strategies based on an accurate assessment of the interactions between cancer biomarkers and NM-based platforms.but are not limited to, carcinoma, sarcoma, leukemia, lymphoma, non-Hodgkin lymphoma, myeloma, central nervous system cancer, lung and bronchus cancer, colon cancer, rectum cancer, prostate cancer, bladder cancer, kidney and renal pelvis cancer, endometrial cancer, pancreatic cancer, liver cancer, and thyroid cancers. [1] Moreover, the most commonly occurring cancers tend to differ by common criteria like sex and age. For example, men are at risk for prostate, lung, and colorectal cancer, women are at risk for breast, lung and colorectal cancer, and children are at risk for leukemia, brain tumors, and lymphoma. The severity of the cancer depends upon the development level or staging of the disease. [2] Likewise, the type of treatment required to treat the cancer is determined by the staging of the disease.The more severe condition of cancer is metastasis, i.e., the uncontrolled growth of cells that invade surrounding tissues. The metastasis condition of cancer can affect any organ of the body. [3] As per the WHO 2010 report on cancer, the lives of 30% patients could have been saved if the cancer was diagnosed earlier. The detection of cancer is not easy because the immune system does not necessarily consider cancerous cells as foreign bodies. However, a few changes (such as altered biomarker levels and the presence of cancerous cells in biological fluids) can be detected. [4] In general, cancer biomarkers are overexpressed proteins present in blood/serum and at the cancer cell surface. The accurate and efficient detection of these biomarkers can be useful for cancer detection. The major problem with biomarkers is their low level in the body during the earlier stages of cancer. Hence, an efficient sensor/device capable of detecting these molecules even at very low levels, if developed, could help the clinician efficiently diagnose cancer cells in the human body.The sensing techniques presently used by clinicians suffer from several limitations; specifically, they are time consuming, bulky, have low sensitivity, and are expensive. Nanomaterial (NM)-based techniques have become an important alternative to the laborious conventional...

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“…It was observed that the quenching intensity increased with the thickness of silver coating and the concentration of CEA. In another recent study, Jeong et al [145] developed GO/AuNP nanocomposite and fluorophore-labeled aptamer for detection of CD44, a cancer biomarker, based on the fluorescence quenching ability of the nanocomposite.…”

Section: The Functions Of Mnps In Various Biosensor Typesmentioning

confidence: 99%

Noble metal nanoparticles in biosensors: recent studies and applications

Malekzad

Zangabad

Mirshekari

et al. 2017

Nanotechnology Reviews

303

119

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The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells. The main readouts are electrochemical (amperometric and voltametric), optical (surface plasmon resonance, colorimetric, chemiluminescence, photoelectrochemical, etc.) and piezoelectric. MNPs have received attention for applications in biosensing due to their fascinating properties. These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility. MNPs can be used alone and in combination with other classes of nanostructures. MNP-based sensors can lead to significant signal amplification, higher sensitivity, and great improvements in the detection and quantification of biomolecules and different ions. Some recent examples of biomolecular sensors using MNPs are given, and the effects of structure, shape, and other physical properties of noble MNPs and nanohybrids in biosensor performance are discussed.

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Robust fluorescence sensing platform for detection of CD44 cells based on graphene oxide/gold nanoparticles

Cited by 22 publications

References 46 publications

Self-Assembling Peptide-Based Multifunctional Nanofibers for Electrochemical Identification of Breast Cancer Stem-like Cells

Self-Assembling Peptide-Based Multifunctional Nanofibers for Electrochemical Identification of Breast Cancer Stem-like Cells

Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Noble metal nanoparticles in biosensors: recent studies and applications

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