Robust gelatin hydrogels for local sustained release of bupivacaine following spinal surgery

Steverink, Jasper G.; Tol, Floris R van; Oosterman, Bas J.; Vermonden, Tina; Verlaan, Jorrit‐Jan; Malda, Jos; Piluso, Susanna

doi:10.1016/j.actbio.2022.05.007

Cited by 11 publications

(13 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bupivacaine can be administered via various routes, including intravenous, epidural, and local infiltration 51 – 53 . Currently, bupivacaine is applied using an encapsulated technique to the polymers to guarantee the long-action analgesic effect 37 , 54 , 55 . In this study, bupivacaine was co-encapsulated with CO 2 into the hydrogel with progressive elution to enhance tendon healing under a continuous analgesic condition.…”

Section: Discussionmentioning

confidence: 99%

Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury

Yu,

Lee,

Hsu

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Nonsurgical treatment and surgical repairment of injured Achilles tendons seldom restore the wounded tendon to its original elasticity and stiffness. Therefore, we hypothesized that the surgically repaired Achilles tendon can achieve satisfactory regeneration by applying multi-drug encapsulated hydrogels. In this study, a novel bupivacaine-eluting carbon dioxide-encapsulated Pluronic F127 hydrogel (BC-hydrogel) was developed for the treatment of Achilles tendon injuries. The rheological properties of BC-hydrogel were measured. A high-performance liquid chromatography assay was used to assess the release characteristics of bupivacaine in both in vitro and in vivo settings. Furthermore, the effectiveness of BC-hydrogel in treating torn tendons was examined in a rat model, and histological analyses were conducted. Evidently, the degradable hydrogels continuously eluted bupivacaine for more than 14 days. The animal study results revealed that the BC-hydrogel improved the post-surgery mobility of the animals compared with pristine hydrogels. Histological assay results demonstrated a significant reaction to high vascular endothelial growth factor in the surrounding tissues and expression of collagen I within the repaired tendon. This demonstrates the potential of this novel BC-hydrogel as an effective treatment method for Achilles tendon injuries.

show abstract

Section: Discussionmentioning

confidence: 99%

Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury

Yu,

Lee,

Hsu

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Using crystallized bupivacaine in making hydrogels can help create a better way to deliver drugs. These hydrogels can release drugs slowly and steadily, which means patients won't need as many injections, making it more comfortable for them [74].…”

Section: By Crystallizationmentioning

confidence: 99%

Advances in Modulating Mechanical Properties of Gelatin-based Hydrogel in Tissue Engineering

Azmir,

Moni,

Gobetti

et al. 2023

Preprint

View full text Add to dashboard Cite

In the last two decades, gelatin-based hydrogels have been widely used as tissue engineering scaffolds due to their excellent biocompatibility, biodegradability, easy processability, transparency, non-toxicity, and reasonable structural similarity to the natural extracellular matrix (ECM). However, intrinsic low mechanical properties of gelatin are not structurally and mechanically suitable to support cell growth and proliferation. That’s why various crosslinking strategies including physical, chemical, enzymatic and combination of them as well as networking patterns including double network, interpenetrating network and nano reinforcing mechanism have been utilized to enhance the structural stability and mechanical integrity of gelatin. In this review, the advances in modulating the mechanical properties of gelatin-based hydrogels for the design and development of structurally stable scaffolds for tissue engineering are discussed. The optimized crosslinking parameters with the adequate mechanical properties of gelatin-based hydrogels are reviewed. Gelatin-based scaffolds for a wide range of tissue engineering applications, such as bone, cartilage, cardiac, skin, and nerve tissue engineering are also outlined. Lastly, current challenges and future perspectives in this research field are presented.

show abstract

“…Such high concentrations are necessary to provide a sufficient dose of local anesthetic for extended durations of analgesia within manageable volumes. One such sustained‐release formulation for use in skeletal surgery is currently under development by the authors, for co‐implantation with screws [13]. However, local toxic effects of bupivacaine concentrations higher than used clinically are largely unknown, and knowledge of these effects is essential to understand the safety of bupivacaine sustained‐release formulations [14].…”

Section: Introductionmentioning

confidence: 99%

Lack of concentration‐dependent local toxicity of highly concentrated (5%) versus conventional 0.5% bupivacaine following musculoskeletal surgery in a rat model

Steverink

Tol

Bruins³

et al. 2023

J. exp. orthop.

Self Cite

View full text Add to dashboard Cite

Purpose Various sustained-release formulations incorporate high bupivacaine concentrations but data on local toxicity is lacking. This study explores local toxic effects of highly concentrated (5%) bupivacaine compared to clinically used concentrations in vivo following skeletal surgery, to assess the safety of sustained-release formulations with high bupivacaine concentrations. Methods Sixteen rats underwent surgery, in which screws with catheters affixed were implanted in the spine or femur in a factorial experimental design, allowing single-shot or continuous 72 h local administration of 0.5%, 2.5% or 5.0% bupivacaine hydrochloride. During the 30-day follow-up, animal weight was recorded and blood samples were obtained. Implantation sites underwent histopathological scoring for muscle damage, inflammation, necrosis, periosteal reaction/thickening and osteoblast activity. Effects of bupivacaine concentration, administration mode and implantation site on local toxicity scores were analyzed. Results Chi-squared tests for score frequencies revealed a concentration-dependent decrease in osteoblast count. Moreover, spinal screw implantation led to significantly more muscle fibrosis but less bone damage than femoral screw implantation, reflecting the more invasive muscle dissection and shorter drilling times related to the spinal procedure. No differences between bupivacaine administration modes regarding histological scoring or body weight changes were observed. Weight increased, while CK levels and leukocyte counts decreased significantly during follow-up, reflecting postoperative recovery. No significant differences in weight, leukocyte count and CK were found between interventional groups. Conclusion This pilot study found limited concentration-dependent local tissue effects of bupivacaine solutions concentrated up to 5.0% following musculoskeletal surgery in the rat study population.

show abstract

Robust gelatin hydrogels for local sustained release of bupivacaine following spinal surgery

Cited by 11 publications

References 59 publications

Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury

Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury

Advances in Modulating Mechanical Properties of Gelatin-based Hydrogel in Tissue Engineering

Lack of concentration‐dependent local toxicity of highly concentrated (5%) versus conventional 0.5% bupivacaine following musculoskeletal surgery in a rat model

Contact Info

Product

Resources

About