Robust, High-Throughput Solution for Blood Group Genotyping

Abstract

With the concomitant increase of blood transfusions and safety rules, there is a growing need to integrate high-throughput and multiparametric assays within blood qualification centers. Using a robust and automated solution, we describe a new method for extended blood group genotyping (HiFi-Blood 96) bringing together the throughput possibilities of complete automation and the microarray multiplexed analysis potential. Our approach provides a useful resource for upgrading blood qualification center facilities. A set of six single-nucleotide polymorphisms (SNPs) associated with clinically important blood group antigens (Kell, Kidd, Duffy, and MNS systems) were selected and the corresponding genotyping assays developed. A panel of 293 blood samples was used to validate the approach. The resulting genotypes were compared to phenotypes previously determined by standard serologic techniques, and excellent correlations were found for five SNPs out of six. For the Kell, Kidd, Duffy, and MNS3/MNS4 systems, high matching percentages of 100%, 98.9%, 97.7%, and 97.4% were obtained, respectively, whereas a concordance percentage of 83.3% only was attained for the MNS1/MNS2 polymorphism.

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

“…Among these, polymerase chain reaction (PCR) sequence-specific primers and multiplex PCR are thought to be successful, offering a suitable quantity for the antigen and enabling multiplex detection. [11][12][13][14] Moreover, they provided a complementary tool for patients with blood groups that are difficult to identify using serological methods. However, the clinical application of PCR technology was limited by a high standard laboratory and lengthy procedure.…”

Identification of Novel Single Nucleotide Polymorphisms Associated with Acute Respiratory Distress Syndrome by Exome-Seq

“…XK is associated with McLeod syndrome (characterized by late-onset abnormalities in the central nervous system and neuromuscular system) and red cell acanthocytosis [33]. XKR3 has previously been indicated as a potential biomarker for blood transfusion compatibility [40]. While it is currently unknown what underlies the association of rs9605146 with susceptibility in ARDS, it causes a deleterious amino acid coding change from proline to leucine in the XKR3 gene as predicted by Provean (score = −5.494).…”

Recent Advances in Multiplex Immunoassays

“…For example, a recent study proposed a microarray-based platform for multiparametric and high-throughput filtration assays (HiFi-assays) [33]. The multiparametric performances of microarray technology are combined with a 96-well format standard; each bottom well supporting a microarray.…”