Abstract-In this paper, the problem of recovering a highresolution frame from a sequence of low-resolution frames is considered. High-resolution reconstruction process highly depends on image registration step. Typical resolution enhancement techniques use global motion estimation technique. However, in general, video frames cannot be related through global motion due to the arbitrary individual pixel movement between frame pairs. To overcome this problem, we propose to employ segmentation-based optical flow estimation technique for motion estimation with a modified model for frame alignment. To do that, we incorporate the segmentation with the optical flow estimation in two-stage optical flow estimation. In the first stage, a reference image is segmented into homogeneous regions. In the second stage, the optical flow is estimated for each region rather than pixels or blocks. Then, the frame alignment is accomplished by optimizing the cost function that consists of L 1 -norm of the difference between the interpolated low-resolution (LR) frames and the simulated LR frames. The experimental results demonstrate that using segmentation-based optical flow estimation in motion estimation step with the modified alignment model works better than other motion models such as affine, and conventional optical flow motion models.