Robust Optimization of Alginate-Carbopol 940 Bead Formulations

López-Cacho, José Manuel; González‐R, Pedro L.; Talero, B.; Rabasco, A. M.; González-Rodrı́guez, Marı́a Luisa

doi:10.1100/2012/605610

Cited by 9 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OED was a central part of this study since it determines the level status of each factor and the interaction between, determining their optimal combination with a minimal number of sampling tests ( López-Cacho et al, 2012 ; Zhou et al, 2012 ). We first used L9 (3 3 ) array to test the inhibitory effects of the three drugs at three concentrations on the proliferation of A-549 cells.…”

Section: Discussionmentioning

confidence: 99%

Optimization of a tri-drug treatment against lung cancer using orthogonal design in preclinical studies

Tan

Wang

Song

et al. 2023

PeerJ

View full text Add to dashboard Cite

A growing body of evidence suggests that anesthetics impact the outcome of patients with cancer after surgical intervention. However, the optimal dose and underlying mechanisms of co-administered anesthetics in lung tumor therapy have been poorly studied. Here, we aimed to investigate the role of combined anesthetics propofol, sufentanil, and rocuronium in treating lung cancer using an orthogonal experimental design and to explore the optimal combination of anesthetics. First, we evaluated the effects of the three anesthetics on the proliferation and invasion of A-549 cells using Cell Counting Kit 8 and Transwell migration and invasion assays. Subsequently, we applied the orthogonal experimental design (OED) method to screen the appropriate concentrations of the combined anesthetics with the most effective antitumor activity. We found that all three agents inhibited the proliferation of A-549 cells in a dose- and time-dependent manner when applied individually or in combination, with the highest differences in the magnitude of inhibition occurring 24 h after combined drug exposure. The optimal combination of the three anesthetics that achieved the strongest reduction in cell viability was 1.4 µmol/L propofol, 2 nmol/L sufentanil, and 7.83 µmol/L rocuronium. This optimal 3-drug combination produced a more beneficial result at 24 h than either single drug. Our results provide a theoretical basis for improving the efficacy of lung tumor treatment and optimizing anesthetic strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Optimization of a tri-drug treatment against lung cancer using orthogonal design in preclinical studies

Tan

Wang

Song

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

“…In the first set of experiments, it was investigated whether the DGSN decoction contained the ferulic acid, peoniflorin, cinnamic acid and glycyrrhizic acid (FPCG) combinations and, if so, whether these active components were absorbed into the blood following oral administration of the DGSN decoction. Animal experiments were then performed based on an L 16 (4 4 ) orthogonal design ( 24 – 26 ), setting four factors with four different levels ( Table II ).…”

Section: Methodsmentioning

confidence: 99%

Protective effect of the combinations of glycyrrhizic, ferulic and cinnamic acid pretreatment on myocardial ischemia-reperfusion injury in rats

Gao

Hao

Hong-kao

et al. 2014

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

The aim of this study was to find an effective drug cocktail pretreatment to protect myocardial tissue of the heart from ischemia-reperfusion (I/R) injury. The mechanisms underlying the effects of the drug cocktail were subsequently explored in order to expand the application of Dang-gui-si-ni-tang (DGSN), a Traditional Chinese Medicine. The active components of DGSN in the serum following oral administration were investigated using high-performance liquid chromatography. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) levels were then analyzed to show the effect of the active components in the treatment of myocardial I/R injury. An L16 (44) orthogonal experiment was utilized to determine the most effective cocktail mix and the mechanism underlying the effect of this mix on myocardial I/R injury was investigated. It was observed that FCG, a mixture of glycyrrhizic (50 mg/kg), cinnamic (200 mg/kg) and ferulic (300 mg/kg) acid, was the optimal drug cocktail present in DGSN. This was absorbed into the blood following oral administration and was shown to decrease MDA levels and increase the activity of SOD. In conclusion, the findings suggest that FCG, a combination of active ingredients in the DGSN decoction, can be absorbed into the blood and protect the myocardium from I/R injury.

show abstract

“…Second, the gel porosity which could result in a burst release of the loaded drug 14 15 . Therefore, many efforts are being currently made to improve the performance of Ca-sodium alginate hydrogel beads as controlled drug delivery carriers 15 . The attempts include the addition of other polymers, such as, chitosan and sodium carboxymethyl cellulose (Na CMC) 16 .…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Hydrogel Beads for Controlled Release of Amoxicillin

Almasri

Swed

Alali

2022

Jordan j. pharm. sci.

View full text Add to dashboard Cite

Amoxicillin trihydrate-loaded hydrogel beads were prepared and characterized as a controlled drug delivery system to improve patient compliance. An ionotropic gelation process was used to prepare the hydrogel beads using calcium chloride (CaCl2) as a crosslinking agent. The effects of CaCl2, sodium alginate, poloxamer 407 (PL) concentration, and preparation temperature were investigated. Spherical hydrogel beads were obtained with high encapsulation efficiency (85.74±1.09) %. FTIR analyses confirmed the compatibility of amoxicillin with the used excipients. In vitro swelling and cumulative drug release studies were performed over 24 hours in HCl medium, pH 1.2. Prepara-tion temperature was found to influence both the index of beads swelling (SI) and the cumulative release of amoxicil-lin. The study demonstrated the capability of PL to enhance the cumulative release of amoxicillin, correlating with swelling behavior. The proposed hydrogel beads have the potential as a promising drug delivery system for controlled release of amoxicillin, over 24 hours, and thus reduced dosing frequency.

show abstract

Robust Optimization of Alginate-Carbopol 940 Bead Formulations

Cited by 9 publications

References 54 publications

Optimization of a tri-drug treatment against lung cancer using orthogonal design in preclinical studies

Optimization of a tri-drug treatment against lung cancer using orthogonal design in preclinical studies

Protective effect of the combinations of glycyrrhizic, ferulic and cinnamic acid pretreatment on myocardial ischemia-reperfusion injury in rats

Preparation and Characterization of Hydrogel Beads for Controlled Release of Amoxicillin

Contact Info

Product

Resources

About