The aim of this research was to develop and validate a questionnaire to assess the quality of education, from a sustainability perspective, in higher education institutions of Naples (Italy), and determine whether differences exist between the groups (families, teachers and, students) with respect to their level of satisfaction concerning that quality, since it is important that education policies promote that assessment from an economic, social, environmental, and organisational dimension. The questionnaire was validated using a quantitative, quasi experimental, cross sectional method. A multivariate analysis of variance (MANOVA) was conducted on a sample of n = 1091 subjects to study the differences between the groups and their level of satisfaction. The results show a good reliability and validity. In this MANOVA, significant differences and large effect sizes were found. Families and teachers consider that quality management in higher education is adequate. The three groups are very satisfied with teaching management, and the teachers’ group has the higher level of satisfaction with teaching planning. It is concluded that this validated questionnaire provides information on the opinions about the quality of education from a sustainability perspective and that the self-assessment of those responsible for teaching is essential to achieve continuous improvement.