SUMMARYA multi-input-multi-output extension of the well-known two control degrees-of-freedom disturbance observer architecture that decouples the problem into single-input-single-output disturbance observer loops is presented in this paper. Robust design based on mapping D-stability and the frequency domain specifications of weighted sensitivity minimization and phase margin bound to a chosen controller parameter space is presented as a part of the proposed design approach. The effect of the choice of disturbance observer Q filter on performance is explained with a numerical example. This is followed by the use of structured singular values in the robustness analysis of disturbance observer controlled systems subject to structured, real parametric and mixed uncertainty in the plant. A design and simulation study based on a four wheel active car steering control example is used to illustrate the methods presented in the paper.