Role and In Vivo Localization of Fusarium oxysporum f. sp. strigae and Bacillus subtilis in an Integrated Striga hermonthica Biocontrol System

Anteyi, Williams Oyifioda; Rasche, Frank

doi:10.1094/phytofr-08-20-0011-r

Cited by 3 publications

(4 citation statements)

References 55 publications

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“…Sorghum (Sorghum bicolor L. Moench) seeds (cultivar PI563294) were sampled from Maradi, Niger. Our previous research studies confirmed the susceptibility of this sorghum cultivar to S. hermonthica [5,6]. The S. hermonthica and sorghum seeds were separately surface-sterilized using sodium hypochlorite, Tween 20 ® and double distilled water (ddH 2 O), then air-dried, according to the procedure of Anteyi and Rasche [5,6].…”

Section: Methodsmentioning

confidence: 63%

“…strigae (Fos) is a well-known bioherbicide (mycoherbicide), that has proven effective for controlling parasitic Striga spp., under both natural and artificial environments [52,58,64]. Although, various Fos isolates with significant pathogenicity towards S. hermonthica have been reported [82], in some cases, the bioherbicidal effectiveness of Fos isolates against S. hermonthica has shown to be inconsistent [6,7,76]. The inconsistent effectiveness of Fos isolates against differing S. hermonthica populations is, amongst others, a major challenge that deters the widespread application of any bioherbicide against its target weed [18,26].…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately, the potential of these latter Fusarium exometabolites to inhibit S. hermonthica seed germination or incidence is not known. Thus, the identification of highly bioactive Fusarium exometabolites with broadscale efficacy against diverse S. hermonthica populations has been suggested as a promising direction for overcoming the limitations of the inconsistent effectiveness of Fos isolates against S. hermonthica [6]. In fact, fungal exometabolites, especially mycotoxins, play important roles in shaping the soil microbial community dynamics.…”

Section: Introductionmentioning

confidence: 99%

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Diacetoxyscirpenol, a Fusarium exometabolite, prevents efficiently the incidence of the parasitic weed Striga hermonthica

2022

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Background Certain Fusarium exometabolites have been reported to inhibit seed germination of the cereal-parasitizing witchweed, Striga hermonthica, in vitro. However, it is unknown if these exometabolites will consistently prevent S. hermonthica incidence in planta. The study screened a selection of known, highly phytotoxic Fusarium exometabolites, in identifying the most potent/efficient candidate (i.e., having the greatest effect at minimal concentration) to completely hinder S. hermonthica seed germination in vitro and incidence in planta, without affecting the host crop development and yield. Results In vitro germination assays of the tested Fusarium exometabolites (i.e., 1,4-naphthoquinone, equisetin, fusaric acid, hymeglusin, neosolaniol (Neo), T-2 toxin (T-2) and diacetoxyscirpenol (DAS)) as pre-Striga seed conditioning treatments at 1, 5, 10, 20, 50 and 100 µM, revealed that only DAS, out of all tested exometabolites, completely inhibited S. hermonthica seed germination at each concentration. It was followed by T-2 and Neo, as from 10 to 20 µM respectively. The remaining exometabolites reduced S. hermonthica seed germination as from 20 µM (P < 0. 0001). In planta assessment (in a S. hermonthica-sorghum parasitic system) of the exometabolites at 20 µM showed that, although, none of the tested exometabolites affected sorghum aboveground dry biomass (P > 0.05), only DAS completely prevented S. hermonthica incidence. Following a 14-d incubation of DAS in the planting soil substrate, bacterial 16S ribosomal RNA (rRNA) and fungal 18S rRNA gene copy numbers of the soil microbial community were enhanced; which coincided with complete degradation of DAS in the substrate. Metabolic footprinting revealed that the S. hermonthica mycoherbicidal agent, Fusarium oxysporum f. sp. strigae (isolates Foxy-2, FK3), did not produce DAS; a discovery that corresponded with underexpression of key genes (Tri5, Tri4) necessary for Fusarium trichothecene biosynthesis (P < 0.0001). Conclusions Among the tested Fusarium exometabolites, DAS exhibited the most promising herbicidal potential against S. hermonthica. Thus, it could serve as a new biocontrol agent for efficient S. hermonthica management. Further examination of DAS specific mode of action against the target weed S. hermonthica at low concentrations (≤ 20 µM), as opposed to non-target soil organisms, is required.

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Section: Methodsmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diacetoxyscirpenol, a Fusarium exometabolite, prevents efficiently the incidence of the parasitic weed Striga hermonthica

2022

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“…The AMF enhance cereal growth to withstand Striga damage and facilitate host plant's uptake of micronutrients, water, and phosphorus (P) from soil through extraradical fungal hyphae (Olowe et al, 2018). The strigolactones (SLs) exudation by the host in the soil is ultimately reduced by the increased uptake of P via symbiotic interactions by AM fungi, thereby reducing Striga infection (Aquino et al, 2021). Furthermore, rate of Striga infestation is equally lowered by the soil microbes through their release of amino acids such as methionine or by the producing secondary metabolites such as trichothecenes which may interfere with SL perception.…”

Section: Current Trends In Striga Managementmentioning

confidence: 99%

Trenchant microbiological-based approach for the control of Striga: Current practices and future prospects

Olowe

Akanmu

Fadiji

et al. 2023

Front. Sustain. Food Syst.

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Striga species are obligate parasitic weeds most of which are members of the Orobanchaceae family. They are commonly associated with staple crops and constitute threats to food security, especially in Sub-Saharan Africa. They pose deleterious impacts on staple cereal crops like maize and pearl millet, resulting in 7–10 billion dollars yield losses or, in extreme infestations, entire crop losses. Farmers' limited knowledge about the weed (genetics, ecology, nature of the damage caused, complex life cycle, interactions with its host and associated microbes) and their attitude toward its control have negatively affected its management and sustainability. With the present Striga management such as mechanical, chemicals, cultural and biological measures, it is extremely difficult to achieve its active management due to nature of the association between host plants and parasites, which requires highly selective herbicides. The use of soil microbes has not been well explored in the management of Striga infection in African countries. However, many soil microorganisms have been considered viable biological control techniques for fighting parasitic weeds, due to their vast action and roles they play in the early stage of host-Striga interaction. Their application for pest control is well perceived to be cost-effective and eco-friendly. In this review, we gave a comprehensive overview of major knowledge gaps and challenges of smallholders in Striga management and highlighted major potentials of microbial-based approach with respect to the mechanisms of host-Striga-microbe interactions, and the metagenomics roles on Striga management that include understanding the microbe and microbial systems of Striga-infested soil.

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Inhibitory activity of bacterial lipopeptides against Fusarium oxysporum f.sp. Strigae

Assena,

Pfannstiel,

Rasche

2024

BMC Microbiol

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This study investigated the influence of bacterial cyclic lipopeptides (LP; surfactins, iturins, fengycins) on microbial interactions. The objective was to investigate whether the presence of bacteria inhibits fungal growth and whether this inhibition is due to the release of bacterial metabolites, particularly LP. Selected endophytic bacterial strains with known plant-growth promoting potential were cultured in the presence of Fusarium oxysporum f.sp. strigae (Fos), which was applied as model fungal organism. The extracellular metabolome of tested bacteria, with a focus on LP, was characterized, and the inhibitory effect of bacterial LP on fungal growth was investigated. The results showed that Bacillus velezensis GB03 and FZB42, as well as B. subtilis BSn5 exhibited the strongest antagonism against Fos. Paraburkholderia phytofirmans PsJN, on the other hand, tended to have a slight, though non-significant growth promotion effect. Crude LP from strains GB03 and FZB42 had the strongest inhibitory effect on Fos, with a significant inhibition of spore germination and damage of the hyphal structure. Liquid chromatography tandem mass spectrometry revealed the production of several variants of iturin, fengycin, and surfactin LP families from strains GB03, FZB42, and BSn5, with varying intensity. Using plate cultures, bacillomycin D fractions were detected in higher abundance in strains GB03, FZB42, and BSn5 in the presence of Fos. Additionally, the presence of Fos in dual plate culture triggered an increase in bacillomycin D production from the Bacillus strains. The study demonstrated the potent antagonistic effect of certain Bacillus strains (i.e., GB03, FZB42, BSn5) on Fos development. Our findings emphasize the crucial role of microbial interactions in shaping the co-existence of microbial assemblages.

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Role and In Vivo Localization of Fusarium oxysporum f. sp. strigae and Bacillus subtilis in an Integrated Striga hermonthica Biocontrol System

Cited by 3 publications

References 55 publications

Diacetoxyscirpenol, a Fusarium exometabolite, prevents efficiently the incidence of the parasitic weed Striga hermonthica

Diacetoxyscirpenol, a Fusarium exometabolite, prevents efficiently the incidence of the parasitic weed Striga hermonthica

Trenchant microbiological-based approach for the control of Striga: Current practices and future prospects

Inhibitory activity of bacterial lipopeptides against Fusarium oxysporum f.sp. Strigae

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