Role and mechanism of cell-surface hydrophobicity in the adaptation of Sphingobium hydrophobicum to electronic-waste contaminated sediment

Chen, Xingjuan; Song, Da; Xu, Jingjing; Li, Enze; Sun, Guoping; Xu, Meiying

doi:10.1007/s00253-017-8734-2

Cited by 16 publications

(13 citation statements)

References 47 publications

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“…Sphingobium hydrophobicum C1 T (= CCTCC AB 2015198 = KCTC 42740) was isolated from e-waste contaminated sediment in Guiyu, China (Chen et al, 2016). Strain C2, a hydrophilic variant of strain C1 T , was obtained by cell passage for ∼100 generations in LB medium (Chen et al, 2017). Strains C3 and C4 were isolated from the first-round subculture (within 10 generations) of strains C1 T and C2 in LB medium, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The CSH of cells in the late-exponential growth phase was measured using the microbial adhesion to hydrocarbon (MATH) protocol as described previously (Chen et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…Strain C1 T is the most hydrophobic sphingomonad ever known. Its high cell surface hydrophobicity (CSH), which may enhance colonization ability and adsorption of hydrophobic nutrients, is greatly attributed to the increased expression of certain outer membrane proteins (Chen et al, 2017). This study aims to address (i) what metabolic potential and genes enable strain C1 T to adapt to the stress from e-waste contaminated sediment?…”

Section: Introductionmentioning

confidence: 99%

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Adaptive Evolution of Sphingobium hydrophobicum C1T in Electronic Waste Contaminated River Sediment

Song

Chen

et al. 2019

Front. Microbiol.

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Electronic waste (e-waste) has caused a severe worldwide pollution problem. Despite increasing isolation of degradative microorganisms from e-waste contaminated environments, the mechanisms underlying their adaptive evolution in such habitats remain unclear. Sphingomonads generally have xenobiotic-degrading ability and may play important roles in bioremediation. Sphingobium hydrophobicum C1T, characterized with superior cell surface hydrophobicity, was recently isolated from e-waste contaminated river sediment. To dissect the mechanisms driving its adaptive evolution, we evaluated its stress resistance, sequenced its genome and performed comparative genomic analysis with 19 other Sphingobium strains. Strain C1T can feed on several kinds of e-waste-derived xenobiotics, exhibits a great resistance to heavy metals and possesses a high colonization ability. It harbors abundant genes involved in environmental adaptation, some of which are intrinsic prior to experiencing e-waste contamination. The extensive genomic variations between strain C1T and other Sphingobium strains, numerous C1T-unique genes, massive mobile elements and frequent genome rearrangements reflect a high genome plasticity. Positive selection, gene duplication, and especially horizontal gene transfer drive the adaptive evolution of strain C1T. Moreover, presence of type IV secretion systems may allow strain C1T to be a source of beneficial genes for surrounding microorganisms. This study provides new insights into the adaptive evolution of sphingomonads, and potentially guides bioremediation strategies.

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Section: Methodsmentioning

confidence: 99%

“…The CSH of cells in the late-exponential growth phase was measured using the microbial adhesion to hydrocarbon (MATH) protocol as described previously (Chen et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adaptive Evolution of Sphingobium hydrophobicum C1T in Electronic Waste Contaminated River Sediment

Song

Chen

et al. 2019

Front. Microbiol.

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“…When other carbon sources were present, phenol addition mostly caused an increase in CSH, regardless of the cells' surface being primarily hydrophobic or hydrophilic, but the observed changes were smaller for P. plecoglossicida IsA. Chen et al [47] have demonstrated that Sphingobium hydrophobicum C1 cells with high cell surface hydrophobicity exposed to deca-bromophenyl ether remained hydrophobic, while the hydrophilic Sphingobium hydrophobicum C2 remained hydrophilic.…”

Section: Cell Membrane Permeability and Surface Hydrophobicitymentioning

confidence: 99%

Bacterial Biodegradation of 4-Monohalogenated Diphenyl Ethers in One-Substrate and Co-Metabolic Systems

et al. 2018

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The use of diphenyl ether (DE) and its 4-monohalogenated derivatives (4-HDE) as flame retardants, solvents, and substrates in biocide production significantly increases the risk of ecosystem contamination. Their removal is important from the point of view of environmental protection. The aim of this study was to evaluate the degradation processes of DE and 4-HDE by enzymes of the environmental bacterial strains under one-substrate and co-metabolic conditions. The study is focused on the biodegradation of DE and 4-HDE, the enzymatic activity of microbial strains, and the cell surface properties after contact with compounds. The results show that the highest biodegradation (96%) was observed for 4-chlorodiphenyl ether in co-metabolic culture with P. fluorescens B01. Moreover, the activity of 1,2-dioxygenase during degradation of 4-monohalogenated diphenyl ethers was higher than that of 2,3-dioxygenase for each strain tested. The presence of a co-substrate provoked changes in dioxygenase activity, resulting in the increased activity of 1,2-dioxygenase. Moreover, the addition of phenol as a co-substrate allowed for increased biodegradation of the diphenyl ethers and noticeable modification of the cell surface hydrophobicity during the process. All observations within the study performed have led to a deeper understanding of the contaminants’ biodegradation processes catalyzed by environmental bacteria.

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“…It exhibits great resistance to multiple heavy metals and can degrade common organic pollutants, such as phthalate esters, diphenyl ether, biphenyl, and chlorobenzene ( 3 ). Compared with its hydrophilic variant C2 obtained by continuous subculture, it has a higher colonization ability and more adsorption capacity for hydrophobic pollutants ( 3 , 20 ). The upregulated expression of some outer membrane proteins in strain C1 detected by comparative proteome analysis may be a key hydrophobic factor ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

The variations of native plasmids greatly affect the cell surface hydrophobicity of sphingomonads

Song,

Chen,

Yao

et al. 2023

mSystems

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The organic pollutant-degrading microorganisms with high cell surface hydrophobicity (CSH) are generally favorable due to the positive role of high CSH in pollutant capture and cell colonization. Sphingomonads, an important bacterial group with metabolic versatility, have significant potential for biodegradation and bioremediation of organic pollutants and generally harbor higher CSH than typical Gram-negative bacteria. However, the molecular mechanisms underlying their high CSH are still unclear. In this study, Sphingobium xenophagum C1, the most hydrophobic sphingomonad ever known, and its hydrophilic variant C2 were used to identify the genome variations responsible for the CSH difference by comparative genome and transcriptome analysis, as well as gene knockout verification. Our results indicated that the high CSH of strain C1 was largely attributed to the low copy number of the native plasmid p3, which mainly affected the transcriptional levels of outer membrane protein genes through direct and indirect means. In addition, loss of the genes on the native plasmid p5 involved in polysaccharide synthesis and secretion could increase CSH and cell surface friction. The bioinformatics analysis further revealed that some sphingomonad genomes also contained the long homologous fragments and/or key genes of p3 and p5. This study demonstrated the important role of native plasmids in regulating the CSH of sphingomonads, suggesting a novel CSH regulation strategy and evolution process. IMPORTANCE Microbial cell surface hydrophobicity (CSH) reflects nonspecific adhesion ability and affects various physiological processes, such as biofilm formation and pollutant biodegradation. Understanding the regulation mechanisms of CSH will contribute to illuminating microbial adaptation strategies and provide guidance for controlling CSH artificially to benefit humans. Sphingomonads, a common bacterial group with great xenobiotic-degrading ability, generally show higher CSH than typical Gram-negative bacteria, which plays a positive role in organic pollutant capture and cell colonization. This study verified that the variations of two native plasmids involved in synthesizing outer membrane proteins and polysaccharides greatly affected the CSH of sphingomonads. It is feasible to control their CSH by changing the plasmid copy number and sequences. Additionally, considering that plasmids are likely to evolve faster than chromosomes, the CSH of sphingomonads may evolve quickly to respond to environmental changes. Our results provide valuable insights into the CSH regulation and evolution of sphingomonads.

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Role and mechanism of cell-surface hydrophobicity in the adaptation of Sphingobium hydrophobicum to electronic-waste contaminated sediment

Cited by 16 publications

References 47 publications

Adaptive Evolution of Sphingobium hydrophobicum C1T in Electronic Waste Contaminated River Sediment

Adaptive Evolution of Sphingobium hydrophobicum C1T in Electronic Waste Contaminated River Sediment

Bacterial Biodegradation of 4-Monohalogenated Diphenyl Ethers in One-Substrate and Co-Metabolic Systems

The variations of native plasmids greatly affect the cell surface hydrophobicity of sphingomonads

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