Role and Mechanism of mir-5189-3p in Deep Vein Thrombosis of Lower Extremities

Lü, Jing; Fang, Qingbo; Ge, Xiaohu

doi:10.1016/j.avsg.2021.07.004

Cited by 10 publications

(3 citation statements)

References 33 publications

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“…Both apoptosis of vascular endothelial cells and the production of inflammatory factors are considered in the development of DVT (32,33). Mounting evidence has emerged that miRNAs, as microregulators, serve a vital role in modulating angiogenic signaling pathways (21,34). In the present study, miR-204-5p was aberrantly low expressed in the serum of DVT patients and could affect apoptosis as well as inflammation in HUVEC through negative regulation of P4HB.…”

Section: Discussionmentioning

confidence: 55%

MiR-204-5p regulates HUVEC cell inflammation and apoptosis by targeting P4HB

Jun Chen,

Kaixin Zhang,

Yi Yang

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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Section: Discussionmentioning

confidence: 55%

MiR-204-5p regulates HUVEC cell inflammation and apoptosis by targeting P4HB

Jun Chen,

Kaixin Zhang,

Yi Yang

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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“…In other words, the aberrant expression of miR-195 and miR-582 can cause endothelial dysfunction by regulating vascular homeostasis and can ultimately lead to thrombus formation in both arteries and veins. Table 2 summarizes the results of other studies that assessed the role of some representative miRNAs in DVT pathogenesis [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61].…”

Section: Mirna and Dvtmentioning

confidence: 99%

MiRNAs and Their Role in Venous Thromboembolic Complications

Gareev,

Pavlov,

et al. 2023

Diagnostics

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Venous thromboembolic complications (VTCs), which include deep vein thrombosis (DVT) and pulmonary embolism (PE), have remained a pressing problem in modern clinical medicine for a long time. Despite the already wide arsenal of modern methods for diagnosing and treating this disease, VTCs rank third in the structure of causes of death among all cardiovascular diseases, behind myocardial infarction (MI) and ischemic stroke (IS). Numerous studies have confirmed the importance of understanding the molecular processes of VTCs for effective therapy and diagnosis. Significant progress has been made in VTC research in recent years, where the relative contribution of microRNAs (miRNAs) in the mechanism of thrombus formation and their consideration as therapeutic targets have been well studied. In this case, accurate, timely, and as early as possible diagnosis of VTCs is of particular importance, which will help improve both short-term and long-term prognoses of patients. This case accounts for the already well-studied circulating miRNAs as non-invasive biomarkers. This study presents currently available literature data on the role of miRNAs in VTCs, revealing their potential as therapeutic targets and diagnostic and prognostic tools for this terrible disease.

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“… 1 DVT, is the world's third most common blood illness, with up to 50 000 new cases per year in the United States and up to 12 million in China. 2 , 3 DVT misdiagnosis is common due to its gradual development and the absence of clinical signs in the early stage. 4 Furthermore, the currently utilized diagnostic biomarker D-dimer is significantly expressed in non-thrombotic illnesses such as stroke, infection, and diffuse vascular coagulation, discounting its specificity.…”

Section: Introductionmentioning

confidence: 99%

LncRNA NEAT1 Promotes Vascular Endothelial Cell Dysfunction via miR-218-5p/GAB2 and Serves as a Diagnostic Biomarker for Deep Vein Thrombosis

Wang

Ren

et al. 2023

Clin Appl Thromb Hemost

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Objective Deep vein thrombosis (DVT) is a common peripheral disease. This study aimed to elucidate the diagnostic biomarker of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in the DVT, and explore possible mechanisms in Human umbilical vein endothelial cells (HUVECs). Methods 101 patients with lower extremity DVT and 82 healthy controls were enrolled. RT-qPCR was designed to resolve the mRNA levels of NEAT1, miR-218-5p, and GAB2. ROC was applied for the diagnosis of DVT. Systemic inflammation (IL-1β, IL-6, and TNF-α) and adhesion factor (SELP, VCAM-1, and ICAM-1) were examined by the ELISA. And cell proliferation, migration, and apoptosis were conducted by the CCK-8, Transwell, flow cytometry assay. The targeting relationship was validated by Dual luciferase reporter and RIP analysis. Results NEAT1 and GAB2 were upregulated in patients with DVT, while miR-218-5p was decreased ( P < .01). Serum NEAT1 can identify DVT patients from healthy individuals. NEAT1 was positively correalted with fibrinolysis factors, coagulation factors, and vasoconstrictors. NEAT1 inhibited the proliferation, migration, and promoted apoptosis as well as inflammation and adhesion factors secretion of HUVECs ( P < .05), but all were impaired by overexpression of miR-218-5p ( P < .05). NEAT1 promoted GAB2 expression in DVT by acting as a sponge for miR-218-5p. Conclusion Elevated NEAT1 is a possible DVT diagnostic biomarker, and is implicated in vascular endothelial cell dysfunction via miR-218-5p/GAB2 axis.

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Role and Mechanism of mir-5189-3p in Deep Vein Thrombosis of Lower Extremities

Cited by 10 publications

References 33 publications

MiR-204-5p regulates HUVEC cell inflammation and apoptosis by targeting P4HB

MiR-204-5p regulates HUVEC cell inflammation and apoptosis by targeting P4HB

MiRNAs and Their Role in Venous Thromboembolic Complications

LncRNA NEAT1 Promotes Vascular Endothelial Cell Dysfunction via miR-218-5p/GAB2 and Serves as a Diagnostic Biomarker for Deep Vein Thrombosis

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